Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
Add more filters










Database
Language
Publication year range
1.
Food Chem ; 406: 135075, 2023 Apr 16.
Article in English | MEDLINE | ID: mdl-36462363

ABSTRACT

Profilin family members are potential pan-allergens in foods, presenting public health hazards. However, studies on the allergenicity modification of profilin allergens are limited. Herein, quercetin and its glycosides (isoquercitrin and rutin) were applied to modify the allergenicity of a profilin allergen (Bra c p) from Brassica campestris bee pollen. Results showed that only quercetin can be closely covalently bound to Bra c p among the three, and the binding site was located at the Cys98 residue. After covalently conjunction, the relative content of α-helix structure in Bra c p was reduced by 40.05%, while random coil was increased by 42.89%; moreover, the Tyr and Phe residues in Bra c p were masked. These structural changes could alter the conformational antigenic epitopes of Bra c p, resulting in its allergenicity reduction. Our findings might provide a technical foundation for reducing the allergenicity of bee pollen and foods containing profilin family allergens.


Subject(s)
Allergens , Pollen , Animals , Bees , Profilins/metabolism , Quercetin/metabolism , Glycosides/metabolism , Immunoglobulin E , Plant Proteins/metabolism
2.
J Agric Food Chem ; 70(50): 15631-15646, 2022 Dec 21.
Article in English | MEDLINE | ID: mdl-36480951

ABSTRACT

Alzheimer's disease (AD) is a neurodegenerative disorder of an ever-increasing aging population with various pathological features such as ß-amyloid (Aß) aggregation, oxidative stress, an impaired cholinergic system, and neuroinflammation. Several therapeutic drugs have been introduced to slow the progression of AD by targeting the above-mentioned pathways. In addition, emerging evidence suggests that naturally occurring compounds have the potential to serve as adjuvant therapies to alleviate AD symptoms. Carotenoids, a group of natural pigments with antioxidative and anti-inflammatory properties, are proposed to be implicated in neuroprotection. To obtain a comprehensive picture of the effect of carotenoids on AD prevention and development, we critically reviewed and discussed recent evidence from in silico, in vitro, in vivo, and human studies in databases including PubMed, Web of Science, Google Scholar, and Cochrane (CENTRAL). After analyzing the existing evidence, we found that high-quality randomized controlled trials (RCTs) are lacking to explore the neuroprotective role of carotenoids in AD pathogenesis and symptoms, especially carotenoids with solid preclinical evidence such as astaxanthin, fucoxanthin, macular carotenoids, and crocin, in order to develop effective preventive dietary supplements for AD patients to ameliorate the symptoms. This review points out directions for future studies to advance the knowledge in this field.


Subject(s)
Alzheimer Disease , Neuroprotective Agents , Humans , Aged , Alzheimer Disease/drug therapy , Alzheimer Disease/prevention & control , Alzheimer Disease/metabolism , Neuroprotective Agents/therapeutic use , Neuroprotective Agents/metabolism , Carotenoids/therapeutic use , Amyloid beta-Peptides/metabolism , Antioxidants/therapeutic use
3.
J Agric Food Chem ; 68(13): 4027-4035, 2020 Apr 01.
Article in English | MEDLINE | ID: mdl-32182051

ABSTRACT

A previous study demonstrated decreased allergenicity in vitro of some food allergens after conjugation with polyphenols. However, little is known about how polyphenol conjugation with food allergens affects in vivo allergenicity. We conjugated a well-known food allergen, ovalbumin (OVA), with quercetin (QUE) to assess the potential allergenicity of OVA in vitro and in vivo in a BALB/c mouse model. QUE could covalently conjugate with OVA and changed the protein structure, which might destroy and/or mask OVA epitopes. Conjugation with QUE decreased IgE binding properties and the release capacity of the conjugated OVA. In vivo, as compared with native protein, conjugation with QUE decreased the levels of IgE, IgG1, IgG, plasma histamine, and mast cell protease-1 (mMCP-1) on the surface of sensitized mast cells, along with decreased FcεRI+ and c-kit+ expression. The levels of Th2-related cytokines (IL-4, IL-5, IL-13) decreased and that of a Th1-related cytokine (IFN-γ) increased slightly, which suggests that conjugation with QUE modulated the imbalance of the Th1/Th2 immune response. Conjugation of OVA with QUE could reduce OVA allergenicity in vitro and in vivo, which could provide information for reducing food allergenicity by conjugation with polyphenols.


Subject(s)
Allergens/immunology , Food Hypersensitivity/immunology , Ovalbumin/immunology , Quercetin/chemistry , Allergens/chemistry , Animals , Cytokines/immunology , Humans , Immunoglobulin E/immunology , Mice , Mice, Inbred BALB C , Ovalbumin/chemistry , Protein Conformation , Th1 Cells/immunology , Th2 Cells/immunology
4.
Food Chem ; 298: 125024, 2019 Nov 15.
Article in English | MEDLINE | ID: mdl-31261007

ABSTRACT

We investigated the allergenicity, digestibility and functional properties of whey protein isolate (WPI) after covalent conjugation with chlorogenic acid (CHA). The covalent conjugation of CHA may cause an unfolded protein structure. The WPI-CHA conjugate showed lower IgE binding capacity but higher intestinal digestibility than unmodified WPI. Furthermore, after digestion, the IgE binding capacity of ß-lactoglobulin and α-lactoalbumin was lower in the digested WPI-CHA conjugate than digested WPI. Moreover, the solubility, emulsifying activity, foaming properties and antioxidant capacity of WPI were enhanced by covalent conjugation of CHA. Covalent conjugation with CHA might reduce the allergenicity in vitro of WPI by improving the functional properties of the protein.


Subject(s)
Allergens/immunology , Whey Proteins/chemistry , Whey Proteins/immunology , Allergens/chemistry , Chlorogenic Acid/chemistry , Digestion , Emulsions/chemistry , Lactoglobulins/chemistry , Solubility
5.
Food Chem ; 281: 28-35, 2019 May 30.
Article in English | MEDLINE | ID: mdl-30658758

ABSTRACT

Anthraquinones, a class of naturally occurring polyphenolic compounds, exhibit a wide range of bioactivities. However, most free anthraquinones are lipophilic bioactive compounds. Bovine ß-lactoglobulin (ßLG), a major whey protein, has a high affinity for small hydrophobic compounds. In this study, the interactions between anthraquinones (rhein, emodin, and chrysophanol) and ßLG were investigated by using fluorescence, circular dichroism (CD), Fourier-transform infrared spectroscopy (FTIR), and docking studies. These anthraquinones bound to the site near Trp19-Arg124 on ßLG with a binding constant (Ka) between 103 and 105 L mol-1 to form complexes, which changed the secondary structure of ßLG, inducing an α-helix to ß-sheet structure transition. The order of binding increased with an increasing polarity in the order of rhein > emodin > chrysophanol. In addition, the degree of radical scavenging capacity masking increased with an increasing binding affinity. Complexation with ßLG significantly increases the hydrosolubility of anthraquinones.


Subject(s)
Anthraquinones/chemistry , Lactoglobulins/chemistry , Polyphenols/chemistry , Animals , Cattle , Circular Dichroism , Emodin/chemistry , Hydrophobic and Hydrophilic Interactions , Models, Theoretical , Molecular Docking Simulation , Spectroscopy, Fourier Transform Infrared , Whey Proteins/analysis
6.
J Agric Food Chem ; 66(37): 9794-9800, 2018 Sep 19.
Article in English | MEDLINE | ID: mdl-30165028

ABSTRACT

Ovalbumin (OVA) is a major allergen in avian egg white. Here, we investigated the conjugation of OVA and chlorogenic acid (CHA) to reduce the allergenic capacity of OVA. OVA-CHA conjugate was characterized by SDS-PAGE, MALDI-TOF-MS, differential scanning calorimetry, and multispectroscopic methods. Sites of the OVA-CHA conjugate were identified by LC-MS/MS. CHA possibly conjugated with Lys20 and Lys17 in OVA, which resulted in the unfolding of OVA. ELISA and Western blot assay indicated that the OVA-CHA conjugate reduced the IgE binding capacity of OVA. The results also indicated that the ability of the OVA-CHA conjugate to activate histamine release was reduced. The decreased allergenic capacity of OVA was attributed to changes in the protein structure. Moreover, the CHA binding site in OVA might directly shield the linear IgE epitope, thereby reducing the IgE binding ability. Also, the OVA-CHA conjugate showed high antioxidant activity. OVA conjugated with CHA may be a promising method of OVA hyposensitization.


Subject(s)
Allergens/chemistry , Chlorogenic Acid/chemistry , Ovalbumin/chemistry , Allergens/immunology , Animals , Chickens , Egg Hypersensitivity/etiology , Egg Hypersensitivity/immunology , Egg Hypersensitivity/prevention & control , Egg White/adverse effects , Egg White/chemistry , Electrophoresis, Polyacrylamide Gel , Histamine Release , Humans , Immunoglobulin E/immunology , Mass Spectrometry , Ovalbumin/immunology
7.
Food Chem ; 256: 427-434, 2018 Aug 01.
Article in English | MEDLINE | ID: mdl-29606470

ABSTRACT

To help produce hypoallergenic food, this study investigated reducing the allergenicity and improving the functional properties of bovine ß-lactoglobulin (ßLG) by covalent conjugation with (-)-epigallo-catechin 3-gallate (EGCG) and chlorogenic acid (CA). The covalent bond between the polyphenols and the amino acid side-chains in ßLG was confirmed by MALDI-TOF-MS and SDS-PAGE. Structural analysis by fluorescence spectroscopy, circular dichroism (CD) and Fourier transform infrared (FTIR) indicated that the covalent conjugate of EGCG and CA led to the changed protein structure of ßLG. Western blot analysis and enzyme-linked immunosorbent assay indicated that conjugation of ßLG with these polyphenols was effective in reducing the IgE-binding capacity of ßLG. The conjugates maintained the retinol-binding activity without denaturation the protein and enhanced the thermal stability with high antioxidant activity. The study provides an innovative approach to producing hypoallergenic food.


Subject(s)
Allergens/chemistry , Lactoglobulins/chemistry , Polyphenols/chemistry , Allergens/immunology , Animals , Catechin/analogs & derivatives , Catechin/chemistry , Cattle , Chlorogenic Acid/chemistry , Circular Dichroism , Electrophoresis, Polyacrylamide Gel , Lactoglobulins/immunology , Spectrometry, Fluorescence
SELECTION OF CITATIONS
SEARCH DETAIL
...