ABSTRACT
OBJECTIVE: To establish HPLC fingerprint of Semen Cassiae. METHODS: Analysis on a Phenomenex C18 (4.6 mm x 250 mm, 5 microm) column eluted with phases containing acetonitrile and 0.1% phosphoric acid in water in gradient mode. The flow rate was 0.8 mL/min and the detection wavelength was 280 nm. The temperature of column was 30 degrees C. Data of 12 batches of Semen Cassiae, ultramicro powder and powder particle from different sources were analysed by "similarity evaluation for chromatographic Fingerprint of Traditional Chinese Medicine" software, and the similarity of them was compared. RESULTS: Twenty-seven common peaks were identified in the chromatograms,and all the common peaks were separated effectively. Fingerprint of ultramicro powder and Semen Cassiae was basic similar, but fingerprint of powder particle and Semen Cassiae was obviously different. CONCLUSION: The method is precise, repeatabe and stable. It can be used to identity and evaluate the quality of Semen Cassiae, and provide important theoretical foundation for clinical applications of Semen Cassiae.
Subject(s)
Cassia/chemistry , Drugs, Chinese Herbal/chemistry , Plants, Medicinal/chemistry , Seeds/chemistry , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal/isolation & purification , Particle Size , Powders/chemistry , Quality Control , Reproducibility of ResultsABSTRACT
OBJECTIVE: To establish HPLC fingerprint of flavonoids and phenols of Dendrobium nobile. METHODS: Phenomenex prodigy ODS(3) C18 column (250 mm x 4.6 mm, 5 microm) was used with a mixture of acetonitrile-0.1% acetic acid as the mobile phase in a gradient mode, the column temperature was 25 degrees C, the flow rate was 1.0 mL/min, and the detection wavelength was 254 nm. RESULTS: The flavonoids and phenols of Dendrobium nobile were well separated, and 10 fingerprint peaks in common were confirmed. CONCLUSION: This method is simple, accurate with good reproducibility, and can be used specifically for the quality control of Dendrobium nobile.
Subject(s)
Chromatography, High Pressure Liquid/methods , Dendrobium/chemistry , Flavonoids/analysis , Phenols/analysis , Plants, Medicinal/chemistry , Drugs, Chinese Herbal/chemistry , Drugs, Chinese Herbal/isolation & purification , Flavonoids/chemistry , Phenols/chemistry , Plant Stems/chemistry , Quality Control , Reproducibility of Results , Solvents/chemistryABSTRACT
OBJECTIVE: To determine the contents of monotropein in the different processed Products of Radix Morindae. CONDITIONS: Kromasil C18 (150 mm x 4. 6 mm,5 micorm) column; The mobile phase was methol-0.4% phosphate solution (5:95-->28.8: 71.2, 15 min); The velocity of flow was 1 ml/min; The detection wavelength was at 210 nm; And column temperature was 25 degrees C RESULTS: There were obvious differences between the crude and kinds of different processed products of monotropein in the Radix Morindae, The contents of monotropein from the different processed products (crude drugs, removed from wood, processed with salt, prepared) were 13.92 mg/g,9.10 mg/g, 9.21 mg/g, 12.86 mg/g, respectively. CONCLUSION: The different processing methods can make the contents of monotropein in the Radix Morindae.
Subject(s)
Iridoids/analysis , Morinda/chemistry , Plants, Medicinal/chemistry , Technology, Pharmaceutical/methods , Chromatography, High Pressure Liquid/methods , Drugs, Chinese Herbal/chemistry , Drugs, Chinese Herbal/isolation & purification , Hot Temperature , Plant Roots/chemistry , Reproducibility of ResultsABSTRACT
OBJECTIVE: To explore the genetic diversity of different farm races of Morinda officinalis on molecular level. METHODS: The molecular biological technique-random amplified polymorphic DNA (RAPD) were used. RESULTS: Of the 40-mer arbitary primers, 14 were found to amplify polymorphic products. 3-5 polymorphic bands were amplified by each polymorphic primer on the average. Using UPGMA method all the tested accesions can be clustered into two groups: 4 accessions of Daye were classified as one group, 1 accession of Xiaoye be another group. CONCLUSION: There actually existed much genetic diversity on molecular level among the different farm races of Morinda officinalis.