ABSTRACT
Oral lichen planus (OLP) is suggested to be a T cell-mediated chronic inflammatory oral mucosal disease. Gene expressed in the oligodendrocyte lineage-myelin basic proteins (Golli-MBP) and stromal interaction molecule 1 (STIM1) are important in the activation and function of T lymphocytes. This study aimed to analyze and compare the expression of Golli-MBP and STIM1 between OLP patients and healthy controls and to analyze the level of intracellular Ca(2+), which is involved in lymphocyte activation. The Ca(2+) fluorescent probe, Fluo-3/AM, was used to test the level of intracellular Ca(2+) in patients with OLP and healthy controls peripheral blood lymphocytes. Golli-MBP and STIM1 mRNA and protein levels were analyzed using quantitative real-time PCR and Western blot, respectively. Following lymphocyte activation, the intracellular Ca(2+) in OLP patients was markedly lower than that in the control group (P < 0.001). In OLP patients, the expression of Golli-MBP mRNA and protein was significantly upregulated compared to those of the control group (P < 0.001). Similarly, OLP patients showed markedly upregulated levels of STIM1 mRNA expression (P < 0.01) and protein compared to healthy controls. The intracellular Ca(2+) of OLP patients was markedly lower than that of healthy controls. This evidence may indicate that Ca(2+) signaling pathways in OLP patients are abnormal. The overexpression of Golli-MBP and STIM1 may play a role in the pathogenesis of OLP.
Subject(s)
Calcium/metabolism , Lichen Planus, Oral/metabolism , Adult , Aged , Case-Control Studies , Female , Gene Expression , Humans , Intracellular Space/metabolism , Lichen Planus, Oral/genetics , Lichen Planus, Oral/immunology , Lymphocyte Activation/genetics , Lymphocyte Activation/immunology , Male , Middle Aged , Myelin Basic Protein/genetics , Myelin Basic Protein/metabolism , Stromal Interaction Molecule 1/genetics , Stromal Interaction Molecule 1/metabolism , T-Lymphocytes/immunology , T-Lymphocytes/metabolism , Young AdultABSTRACT
OBJECTIVE: To examine the effect of mouthwash Yupingfeng on the level of salivary epidermal growth factor (sEGF) in oral lichen planus (OLP). METHODS: The level of sEGF was measured by radioimmunoassay with ligand 125I-EGF. The saliva samples were taken from the normal control group and the OLP patients before and after treatment with Yupingfeng. RESULTS: The levels of sEGF in OLP patients before treatment with Yupingfeng were (4.09 +/- 3.64) microg/L, which was significantly higher than that in normal control [(2.15 +/- 1.62) microg/L, P = 0.013], and (2.57 +/- 1.19) microg/L after treatment,which was significantly lower than before treatment (P = 0.05). CONCLUSIONS: Yupingfeng can modulate the level of sEGF in OLP patients.
