ABSTRACT
Rationale: Aggressive fibromatosis is a rare and locally infiltrative monoclonal fibroblastic proliferation with lack of metastatic potential. We describe a rare case of intra-abdominal aggressive fibromatosis on young female with hyperemesis. Patient concerns: A 23-year-old female was admitted with hyperemesis and loss of weight. Diagnoses: According to imaging findings and immunohistology findings, a diagnosis of intra-abdominal aggressive fibromatosis was formulated. Outcomes: After the surgery, no evidence of local recurrence was noted during the 6 months of follow-up. Lessons: AF may explain why pregnant women may have severe hyperemesis.
ABSTRACT
OBJECTIVES: To compare the expression of the Wnt signaling-associated proteins (Wnt3, ß-catenin, MMP-7) in gastric cancer and precancerous lesions with positive and negative Helicobacter pylori (H.pylori, Hp) infection, and to further explore the mechanisms underlying the Wnt signaling pathway involving in the formation of gastric cancer and its relationship with Hp infection. METHODS: The complete paraffin samples with pathologically confirmed diagnosis, who came from the First Hospital of Changsha from January 2018 to April 2020, were collected. All samples were randomly divided into a gastric cancer group (n=57), a precancerous lesion group (n=84), and a chronic superficial gastritis group (n=25). Improved Giemsa staining was used to detect Hp infection, and according the results of Hp infection the above groups were divided into a Hp positive subgroup and a negative subgroup. The expressions of Wnt3, ß-catenin and MMP-7 were examined with immunohistochemistry. RESULTS: The Wnt3, ß-catenin, and MMP-7 were highly expressed in the gastric cancer group and the gastric precancerous lesion group. The Wnt3 and MMP-7 were highly expressed in cytoplasm, and ß-catenin showed a tendency of cell membrane transferring to cytoplasm and nucleus, which was characterized by "nuclear translocation". The positive rates of the Wnt3, ß-catenin, and MMP-7 expressions in the gastric cancer group were higher than those in the precancerous lesion group and the chronic superficial gastritis group (all P<0.05), which showed a gradually increasing trend with the deterioration of differentiation degree. In addition, the expressions of Wnt3, ß-catenin, and MMP-7 in the Hp positive subgroup in the gastric cancer group and the precancerous lesion group were higher than those in the Hp negative subgroup (all P<0.05). CONCLUSIONS: Aberrant activation of Wnt signaling pathway is involved in the occurrence and development of precancerous lesions and gastric cancer, and which is related with Hp infection. Meanwhile, the Wnt3, ß-catenin and MMP-7 may be used as molecular markers for early diagnosis of gastric cancer and indicators to judge the degree of differentiation and malignancy of gastric cancer.
Subject(s)
Helicobacter Infections , Helicobacter pylori , Precancerous Conditions , Stomach Neoplasms , Gastric Mucosa , Humans , Matrix Metalloproteinase 7/genetics , Wnt3 Protein , beta Catenin/geneticsABSTRACT
To investigate the feasibility of ß-tricalcium phosphate (TCP)/gelatine scaffold combined with allogeneic adipose-derived stem cells (ASCs) to repair hole shape defect, third-passage ASCs were seeded onto composite scaffolds to prepare an ASC-ß-TCP/gelatine tissue-engineered bone to pack into the rabbit cavernous bone defects of experimental groups. In animal models, the bone defect area was completely filled and difficult to recognize in the experimental group at 12 weeks post-surgery by gross observation and radiographic examination. The average bone mineral density value of them was higher than that of the control group. Because of the biocompatibility with allogenic ASCs and the osteoconductivity of ß-TCP/gelatine scaffolds, ß-TCP/gelatine is suitable as a plastic scaffold for the ASC-seeded tissue-engineered bone to repair cavernous defects.
