[Imaging Analysis in Cases with Clonorchiasis-Associated Cholangiocarcinoma].

Objective: To investigate the magnetic resonance imaging （MRI） and computed tomography (CT) features of clonorchiasis-associated cholangiocarcinoma, and provide reference for its clinical diagnosis. Methods: The CT and MRI （including magnetic resonance cholangiopancreatography, MRCP） data of 60 patients diagnosed to have cholangiocarcinoma（26 cases with clonorchiasis-associated cholangiocarcinoma, group A; 34 cases with simple cholangiocarcinomas, group B） by surgery or biopsy in two hospitals in Guangdong Province during July 2005 and June 2015 were collected. The tumor location, pathological types, imaging features, mode of enhancement, and the bile duct expansion were analyzed. Results: Imaging results showed that the tumor tended to occur in the right liver in group A（46.2%, 12/26） and in the left hepatic duct and the liver explorer in group B （61.8%, 21/34）（P<0.05）. The pathological types of tumor in both groups included the nodule/mass type (group A, 73.1%, 19/26; group B, 52.9%, 18/34), the infiltration type （15.4%, 4/26; 23.5%, 8/34）, and the cavity growth type（11.5%, 3/26; 23.5%, 8/34）（P>0.05）. Plain and enhanced CT and MRI results revealed no significant difference in tumor density, signal characteristics or the mode of enhancement between the two groups. MRCP results showed that the intrahepatic distal bile duct cystic dilatation, the intrahepatic bile duct cane soft tubular ectasia, the bile duct dilatation in the tumor, and the bile duct dilatation surrounding the tumor accounted for 61.5%（16/26）, 19.2% （5/26）, 50% （13/26） and 7.7%（2/26） in group A, and 8.8% （3/34）, 64.8% （22/34）, 20.6% （7/34） and 38.2% （13/34） in group B （P<0.05 for each of the four）， respectively. Conclusion: The clonorchiasis-associated cholangiocarcinoma has certain imaging characteristics. It is different from the simple cholangiocarcinomas in tumor location and intrahepatic bile duct dilatation.

Cotton gene expression profiles in resistant Gossypium hirsutum cv. Zhongzhimian KV1 responding to Verticillium dahliae strain V991 infection.

Verticillium wilt of cotton (Gossypium hirsutum) is a widespread and destructive disease that is caused by the soil-borne fungus pathogen Verticillium dahliae (V. dahliae). To study the molecular mechanism in wilt tolerance, suppression subtractive hybridization (SSH) and dot blot techniques were used to identify the specifically expressed genes in a superior wilt-resistant cotton cultivar (G. hirsutum cv. Zhongzhimian KV1) after inoculation with pathogen. cDNAs from the root tissues of Zhongzhimian KV1 inoculated with V. dahliae strain V991 or water mock were used to construct the libraries that contain 4800 clones. Based on the results from dot blot analysis, 147 clones were clearly induced by V. dahliae and selected from the SSH libraries for sequencing. A total of 92 up-regulated and 7 down-regulated non-redundant expressed sequences tags (ESTs) were identified as disease responsive genes and classified into 9 functional groups. Two important clues regarding wilt-resistant G. hirsutum were obtained from this study. One was Bet v 1 family; the other was UbI gene family that may play an important role in the defense reaction against Verticillium wilt. The result from real-time quantitative reverse transcription polymerase chain reaction showed that these genes were activated quickly and transiently after inoculation with V. dahliae.

[A microsatellite marker linked to the stripe rust resistance gene YrV23 in the wheat variety Vilmorin23].

Vilmorin23 is an internationally used differential host variety for studies on the interactions between wheat stripe rust and wheat. It contains the stripe rust resistance gene YrV23 and is potentially an important source of stripe rust resistance worldwide. SSR analysis was performed on the wheat NIL Taichuang 29*6/YrV23 carrying the resistant gene YrV23 against stripe rust, Vilmorin 23 and its recurrent parent Taichung 29. Fifty pairs of SSR primers on wheat chromosome 2B were screened and a reproducible polymorphic DNA fragment amplified by Xwmc356 was found. Genetic linkage was tested on 150 segregating F2 plants. It showed that the microsatellite marker Xwmc356 was linked to the resistance gene YrV23 with a genetic distance of 9.4 cM.

[Microsatellite marker linked with stripe rust resistant gene Yr9 in wheat].

SSR analysis was performed using a wheat near-isogenic line (NIL) Taichuang29 * 6/ Lovrin13, which carried the resistance gene Yr9 against wheat stripe rust and its recurrent parent Taichung29 as materials. After screening with 32 SSR primers on 1B chromosome, reproducible polymorphic DNA fragment amplified by Xgwm582 was identified. Genetic linkage was tested in 177 segregating F2 plants. The results indicated that microsatellite marker Xgwm582 was linked with gene Yr9 resistant to wheat stripe rust. A genetic distance of 3. 7 cM was calculated.

[RAPD markers linked to the stripe rust resistance gene Yr5 in the wheat variety Triticum spelta album].

A total of 520 10-mer random primers were used to identify the RAPD markers linked to the Yr5 gene between the near-isogenic line Yr5/6 x Avocet S and recurrent parent Avocet S. Three polymorphic DNA fragments, S1496(761), S1453(880) and S1418(1950), were found linked to the Yr5 gene. In which the genetic distance between S1496(761) and Yr5 gene was 2.7 cM. The fragment S1496(761) was recovered from the gel and cloned and sequenced. A pair of specific PCR primers was designed based on the sequence. The specific primers amplified the same fragment about 761bp as the random primer S1496 did. Because the primers could amplify another non-specific fragment, the PCR products must be analyzed by electrophoresis on polyacrylamide gels.