ABSTRACT
Through the immobilized biosorption of activated carbon and Paenibacillus polymyxa GA1 by sodium alginate, this paper take advantage of the orthogonal experiment to investigate the adsorption characteristics of sodium alginate. According to the research, the optimal preparation conditions were as following: the mass fraction of sodium alginate was 2.5%; the amount of carbon and bacteria was 1:20 and 1:2, respectively; under the preparation condition, the adsorption rate could reach to 93.74%. The adsorption conditions of the immobilized beads were: pH 5, temperature 30 degrees C and Pb2+ with an initial concentration of 300 mg x L(-1). Moreover, the immobilized activated carbon and GA1 improved their application range of pH, temperature and the initial concentration of Pb2+. Based on the equilibrium curve, it showed that the adsorption of Pb2+ was a rapid process within 30 min and gradually reached leveling within 2 h. What's more, the equilibrium curve could be well described by Langmuir model and Freundlich model, which be consisted mainly of monolayer adsorption, and the maximum monolayer adsorption amount was 370.37 mg x g(-1). Finally, the desorption result showed that the immobilized beads could be recycled effectively.
Subject(s)
Alginates/chemistry , Charcoal/chemistry , Environmental Pollutants/isolation & purification , Lead/isolation & purification , Paenibacillus/metabolism , Adsorption , Biodegradation, Environmental , Environmental Pollutants/metabolism , Glucuronic Acid/chemistry , Hexuronic Acids/chemistry , Immobilization , Lead/metabolismABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of an active fraction (DSS-A-N-30) from Danggui Shaoyao San.</p><p><b>METHOD</b>DSS-A-N30 was prepared by macroporous resin chromatography, the compound was isolated by column chromatography on silica gel and RPC-18, the structure was elucidated by spectroscopic methods.</p><p><b>RESULT</b>A new monoterpene glycoside was isolated and identified from DSS-A-N-30.</p><p><b>CONCLUSION</b>The new monoterpene glycoside was identified as 4"-hydroxyl-albiflorin.</p>
Subject(s)
Bridged-Ring Compounds , Chromatography, Gel , Drugs, Chinese Herbal , Chemistry , Magnetic Resonance Spectroscopy , MonoterpenesABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of eleutheroside B in As1 (the extract fraction from Acanthopanax senticosus, which has a good effect of antifatigue).</p><p><b>METHOD</b>The antifatigue effect of Asl was evaluated by mice burden swimming testing. One compound was isolated by silica gel, Sephadex LH-20 column chromatography and preparative high performance liquid chromatography. The structure was identified by physicochemical properties and spectral evidences. Eleutheroside B in Asl was determined by HPLC. Chromatographic conditions included Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) and the mobile phase consisting of a mixture of acetonitrile-water-ethanoic acid (10: 90: 0.01). The UV detection wavelength was set at 344 nm.</p><p><b>RESULT</b>As1 showed an excellent antifatigue activity; One compound was isolated from As1 and its structure was identified as Eleutheroside B; The calibration cure was linear in the range of 0.104-20.8 microg (r = 0.9999), the average recovery was 97.68%, RSD 1.4% (n=6).</p><p><b>CONCLUSION</b>This HPLC method is simple,accurate and reliable.</p>
