Role of menopausal hormone therapy in the prevention of postmenopausal osteoporosis.

The use of menopausal hormone therapy (MHT) has declined due to concerns about its potential side effects. However, its pivotal role in managing postmenopausal osteoporosis is gaining increased recognition. In this article, we explore how MHT assists postmenopausal women in maintaining bone health and preventing fractures. Recent research indicates that MHT significantly reduces the risk of fractures in women. This benefit is evident regardless of a woman's bone mineral density or their use of progestogens. However, there is limited evidence suggesting that the skeletal benefits continue once the treatment is discontinued. Possible complications of MHT include heart attacks, clots, strokes, dementia, and breast cancer. The most suitable candidates for MHT are women who have recently entered menopause, are experiencing menopausal symptoms, and are below 60 years of age with a minimal baseline risk of adverse events. The treatment is available to those who meet these criteria. For women undergoing premature menopause, MHT can be considered as a means to protect bone health, especially if initiated before menopause or if accelerated bone loss is documented soon after menopause. Such decisions should be made after evaluating individual risk factors and benefits.

Screening and Survival Analysis of Hub Genes in Gastric Cancer Based on Bioinformatics.

Screening for hub genes associated with gastric cancer and elucidating possible molecular mechanisms of gastric cancer. Five gastric cancer-related gene expression profiles were extracted from the GEO database, and differentially expressed genes (DEGs) were obtained using GEO2R. Gene ontology (GO) enrichment analyses were performed by DAVID, and protein-protein interaction (PPI) network of the DEGs was constructed by STRING and Cytoscape software. Survival value for hub gene comes from the Kaplan-Meier plotter platform. In addition, potential miRNAs of hub genes were predicted by miRWalk. Four hundred seventy-six DEGs were identified in the five expression profiles, these genes are mainly involved in extracellular matrix (ECM)-receptor interaction, chemical carcinogenesis, gastric acid secretion, and PI3K-Akt signaling pathway. Combined with the results of the PPI network and CytoHubba, six hub genes were screened: SERPINH1, NPY, PTGDR, GPER, ADHFE1, and AKR1C1. These genes are highly expressed in gastric cancer tissues, and the overexpression level of these genes is associated with poor survival. A series of miRNAs such as hsa-miRNA-92a-1, hsa-miRNA-647, and hsa-miRNA-507 may play a key role in hub gene regulation. Our studies indicate that SERPINH1, NPY, PTGDR, GPER, ADHFE1, and AKR1C1 may be potential biomarkers and therapeutic targets for gastric cancer in the future.

Possible genetic reproductive isolation between two tilapiine genera and species: Oreochromis niloticus and Sarotherodon melanotheron.

Successful crossbreeding between Oreochromis niloticus and Sarotherodon melanotheron to produce a commercial hybrid has been difficult. The karyotypes and isoenzyme of these two species and their reciprocal hybrids (O. niloticus female × S. melanotheron male, S. melanotheron female × O. niloticus male, the last not included in the isoenzyme study) were investigated via metaphase chromosomes obtained from head kidney cells and electropherogram of lactate dehydrogenase (LDH) isoenzymes from the liver, kidney, white muscle, heart, and eye balls. The diploid chromosome number (2n=44) and the fundamental number (NF=50) of the four tilapia genotypes were the same. However, the karyotype of O. niloticus had three pairs of sub-metacentric (sm), twelve pairs of sub-telocentric (st), and seven pairs of telocentric (t) chromosomes, while S. melanotheron had one pair of metacentric (m), two pairs of sm, 12 pairs of st, and seven pairs of t chromosomes. The reciprocal hybrids both showed a mixed karyotype range between their parents: 0.5 pair of m, 2.5 pairs of sm, 12 pairs of st, and seven pairs of t chromosomes. In view of the electropherogram of isozymes, only the LDH of the kidney showed significant clear bands, with five bands in O. niloticus, three bands in S. melanotheron, and duplicated six bands in the hybrids. The bands varied depending on their activities and mobilities. We considered that the differences in karyotype and isoenzyme were related to the genetic mechanism for post-mating isolation, and provided some additional basic genetic background of their taxonomy.