Observation on the clinical effect of thunder-fire moxibustion combined with acupressure on ocular muscle spasm: A clinical randomized controlled trial.

INTRODUCTION: With the rapid development of social economy, peoples dependence on computers and mobile phones is increasing day by day. This causes people to often overuse. Therefore, the incidence of Ocular muscle spasm has been increasing year by year in recent years. The disease usually starts and hides, which seriously affects the patients social image, daily life, and work. METHODS/DESIGN: We will compare the clinical efficacy of thunder-fire moxibustion combined with acupressure with pure thunder-fire moxibustion on Ocular muscle spasm using random control method. DISCUSSION: We aim to find a simple, safe, simple and effective Chinese medicine nursing technology that relieves Ocular muscle spasm. TRIAL REGISTRATION: ClinicalTrials.gov,ChiCTR2000034187, Registered on 27 June 2020.

[Gene Analysis of Thalassemia in Han and Dai Ethnic Childbearing-aged Population of Chinese Yunnan Province].

OBJECTIVE: To investigate the common mutation spectrum of α- and ß-thalassemia in Yunnan childbearing-aged population. METHODS: The common mutation types of α- or ß-globin genes were detected by multiple Gap-PCR and the PCR-reversed dot blotting, and the unknown mutation types were determined by DNA sequencing in DNA samples of hypochromic microcytic anemia patients and carriers who were confirmed to be positive by serologic screaning, then the mutation types of globin in Yunnan population were analyzed statistically. RESULTS: A total of 40 kinds of mutation types were detected in 685 detected persons, among them the 3 commonest mutation types of α-globin genes were --(SEA)/αα (49.09%), -α(3.7)/αα (36.67%) and α(CS)α/αα (8.79%), the 3 commonest genetypes of ß-globin gene were CD26(GAG>AAG)/N (43.78%), CD41-42(-CTTT)/N (20.1%) and CD17(AAG>TAG)/N (18.9%). There were 348 Han and 212 Dai ethnic persons in 685 cases, but their mutation of globin genes were different between these 2 ethnic groups. The results also showed that the gene mutation types were mostly concentrated in Dai ethnic individuals, since 28 of 38 detected α-ß-thalassemia cases were Dai ethnic individuals. CONCLUSION: The mutation spectrums of α- and ß-globin genes in Yunnan childbearing-aged population are diverse and different from that in other areas of China.

[Gene Mutation Spectrum of ß-Thalassemia in Dai Ethinic Population of Two Border Region in Chinese Yunnan Province].

OBJECTIVE: To investigate the gene mutation spectrum of ß-thalassemia in Dai ethnic population of 2 border region in Chinese Yunnan Province. METHODS: The patients with ß-thalassemia in Dai ethnic population of Dehong and Xishuangbanna autonamic prefecture were screened by using blood routine detection and capillary electrophoresis. The ß-globin gene mutation in patients with ß-thalassemia were detected by using PCR reverse dot-blot hybridization (PCR-RDB), the constitutive rate of gene mutation in patients with ß-thalassemia of Dai ethnic population in two border regions was analyzed and compared. RESULTS: A total of 186 patients with gene mutation of ß-thalassemia were confirmed. Among them, 10 gene mutation were found, and the 5 main gene mutations were CD26 (62.56%), CD41-42 (18.97%), CD17 (14.36%), CD71-72 (2.05%) and IVS-II-654 (1.54%). Among Dai ethinic population in Dehong region, 4 gene mutations were found including CD26 (80.31%), CD17 (11.02%), CD41-42 (6.30%) and CD71-72 (2.36%). Among Dai ethinic population in Xishuangbanna region, 6 gene mutations were found, out of them the more common gene mutations were CD41-42 (42.64%), CD26 (29.41%) and CD17 (20.59%). CONCLUSION: The gene mutations of ß-thalassemia in Dai ethinic population of Yunnan province has been confirmed to be more genetic heterogenicity, the spectrums of ß-thalassemia mutations in Dai ethinic population of different regions were significant different.

[Content and evolution characteristics of organic carbon associated with particle-size fractions of grey desert soil under long-term fertilization].

Physical fractionation technique was used to analyze the content, distribution, and temporal evolution of organic carbon associated with particle-size fractions of grey desert soil under long-term (1990-2007) fertilization. Compared with no fertilization, a combined application of manure and chemical fertilizers increased the organic carbon associated with particle-size fractions significantly, with the highest increment (0.34 g x kg(-1) x a(-1)) in sand. Following also increased the organic carbon associated with clay and silt significantly. Straw return to cropland only maintained the organic carbon content in different particle-size fractions, while long-term application of chemical fertilizers was not beneficial to the organic carbon increase in particle-size fractions. Coarse silt and coarse clay had the highest distribution rates of organic carbon (27.9% and 27.1%, respectively), being the important fractions in sequestrating organic carbon. When manure was applied with chemical fertilizers, the organic carbon in sand was significantly increased by 119.4%, while that in fine silt and coarse clay was significantly decreased by 40.3% and 37.9%, respectively, which resulted in the increase in the ratio of particulate organic carbon content (W(POC)) to mineral-associated organic carbon content (W(MOC)), and improvd soil carbon property. Long-term application of manure combined with chemical fertilizers was the best mode to increase the organic carbon content in particle-size fractions and improve the fertility of grey desert soil.

[Pathological basis of qi-deficiency and blood stasis syndrome in patients with non-small cell lung cancer viewing from tumor metastasis related factors].

OBJECTIVE: To observe the expressions of tumor metastasis related factors (TMRF) in peripheral blood of patients with non-small cell lung cancer (NSCLC) for exploring the molecular mechanism for genesis of qi-deficiency and blood stasis (QDBS) syndrome in patients. METHODS: Eighty selected NSCLC patients of stage II B/III were differentiated into the QDBS group and the non-QDBS group according to the Chinese medicine syndrome differentiation criteria, 40 in each group. The serum levels of vascular endothelial growth factor (VEGF), endostatin (ES) and soluble intercellular adhesive molecule-1 (slCAM-1) in patients were detected by ELISA, and the expression of adhesive molecule CD44 in peripheral blood was determined using flow cytometry. RESULTS: Serum levels of VEGF (1002.78 +/- 312.08 ng/L), ES (120.88 +/- 20.00 microg/L), slCAM-1 (531.78 +/- 213.37 microg/L) and CD44 (136.65 +/- 29.60) were significantly higher in patients of QDBS group than in patients of non-QDBS group (653.18 +/- 318.99 ng/L, 98.29 +/- 23.92 microg/L, 409.36 +/- 167.65 microg/L and 98.46 +/- 20.64, respectively, P<0.01). CONCLUSION: Objective inner links are found between the QDBS syndrome and TMRF in NSCLC patients; serum levels of VEGF, ES, slCAM-1 and CD44 can be served as the microcosmic basis for QDBS syndrome differentiation.

{µ-6,6'-Dimeth-oxy-2,2'-[propane-1,3-diylbis(nitrilo-methyl-idyne)]-diphenolato}-trinitratocopper(II)lutetium(III) acetone solvate.

In the title complex, [CuLu(C(19)H(20)N(2)O(4))(NO(3))(3)]·CH(3)COCH(3), the Cu(II) ion is four-coordinated in a square-planar geometry by two O atoms and two N atoms from the deprotonated Schiff base. The Lu(III) ion is ten-coordinate, chelated by three nitrate groups and linked to the four O atoms of the deprotonated Schiff base. A mol-ecule of acetone is present as a solvate.

[Effect of heijiang pill on radiation skin ulcer in rats].

OBJECTIVE: To investigate the relationship between single dosage of 60Co radiation and the degree of radiation-induced skin ulcers, and to evaluate the curative effect of Heijiang Pill (HJP) on skin ulcer induced by various dosages of radiation in rats. METHODS: Sixty-six Wistar female rats were randomly divided into three groups, the blank control group (n = 6) and the two radiation groups, each 30 rats, with their right hind leg exposed respectively to 60 Gy and 40 Gy of 60 Co radiation. The time of emergence and degree of skin ulcer were recorded. Then rats in the two radiation groups were subdivided into the HJP group, the Ethacridine group and the model group, 10 in each group, they received corresponding treatment after ulceration, and the incidence, pathology, cure rate and cure time of skin ulcer were observed in the 90 days of observation. RESULTS: The incidence of skin ulcer was higher and occurred earlier in rats radiated with 60 Gy than that with 40 Gy (P < 0.05). In the same radiation dosage, the ulcer healing rate in rats treated with HJP was higher than that treated with Ethacridine (P <0.05), and in the subgroups of 40 Gy radiation, the mean cure time in the HJP group was shorter (P < 0.05). The local epithelial proliferation and repairing could be verified by pathological observation in the HJP group. CONCLUSION: Larger dosage of radiation leads to earlier emergence and aggravation of ulcers. HJP can effectively cure radiation skin ulcer, and the effect is especially significant on the ulcer induced by low dose radiation.

Catalytic, asymmetric aza-Baylis-Hillman reaction of N-sulfonated imines with activated olefins by quinidine-derived chiral amines.

The chiral nitrogen Lewis base, tricyclic cinchona alkaloid derivative TQO, is an effective promoter in the catalytic, asymmetric aza-Baylis-Hillman reaction of N-sulfonated imines Ar-CH=NR' 1 (R' = Ts, Ms, Ns, SES) with various activated olefins such as methyl vinyl ketone (MVK), ethyl vinyl ketone (EVK), acrolein, methyl acrylate, phenyl acrylate, or alpha-naphthyl acrylate to give the corresponding adducts in moderate to good yields with good to high ee (up to 99 %) at -30 degrees C or 45 degrees C in various solvents, including DMF/MeCN (1:1, v/v). The first such reaction of 1 with the simplest Michael acceptor MVK and methyl acrylate has been achieved with excellent enantioselectivity. The adducts derived from MVK and EVK had the opposite absolute configuration to those from acrolein, methyl acrylate, phenyl acrylate, and alpha-naphthyl acrylate. A plausible mechanism has been proposed on the basis of previous reports and the authors' investigations. An effective bifunctional chiral nitrogen Lewis base-Brønsted acid system has been revealed in this type of aza-Baylis-Hillman reaction.

Highly efficient aza-Baylis-Hillman reaction of N-tosylated imines with MVK, acrolein, and phenyl acrylate or alpha-naphthyl acrylate: Lewis base effects and a convenient method to synthesize alpha,beta-unsaturated beta-amino carbonyl compounds.

This paper describes several highly efficient aza-Baylis-Hillman reactions of N-tosylated imines with MVK, acrolein, and phenyl acrylate or alpha-naphthyl acrylate in the presence of a Lewis base. In most cases, the reaction can be completed within 1 h using the appropriate Lewis base catalyst. An efficient method to synthesize beta-amino ketones, aldehydes and esters in high yields and short reaction time has been developed.

An unexpected highly stereoselective double aza-Baylis-Hillman reaction of sulfonated imines with phenyl vinyl ketone.

The aza-Baylis-Hillman reaction of N-sulfonated imine with phenyl vinyl ketone gave the double aza-Baylis-Hillman adduct in good yields with excellent stereoselectivities in the presence of Lewis base 1,4-diazabicyclo[2.2.2]octane.