[Effects of acupoint-injection of Lentinan on the immunologic funtion in rabbits with spleen-qi deficiency].

UNLABELLED: To observe the effect of Spleen-Meridian-acupoint injection of Lentinan on the immunologic function in spleen-deficiency rabbits. METHODS: A total of 54 Newzealand rabbits were randomly divided into normal (n = 10), model (n = 8), intramuscular injection (n = 10), Sanyinjiao (SP6, n = 10), Diji (SP8, n = 8) and Xuehai (SP 12, n = 8) groups. Spleen-qi deficiency model was established by intragastric administration of 100% crude Radix et Rhizoma Rhei decoction (15 mL/kg/day x 10 d), and then Lentinan (LNT, 0.025 mg/kg/2 day x 5) was injected into the aforementioned acupoints of the Spleen Meridian. The erythrocyte immunologic function (RBC-C3 bR, RBC-IC), hemolysin (lgM) and changes of physical signs of the rabbits were observed. RESULTS: In comparison with the control group, the rabbits' body weight, rectal temperature, RBC-C3 bR% and serum IgM level were decreased significantly in model group (P < 0.05); while in comparison with the model group, the body weight in SP 8 group,retal temperature in SP 9 and SP 8 groups, RBC-C3 bR% in SP 9 and SP 12 groups, and serum IgM levels in SP 9, SP 8 and SP 12 groups increased considerably (P < 0.05, P < 0.01). Comparison among the 4 treatment groups showed that the effect of SP 12 was superior to that of intramuscular injection group in upregulaing RBC-C3 bR%, and the effects of SP 9, SP 8 and SP 12 groups were significantly superior to those of intramuscular injection group in upregulating serum IgM level (P < 0.05). CONCLUSION: The Spleen-Meridian-acupoint injection of LNT is superior to that of intramuscular injection of LNT in improving the spleen-qi deficiency rabbits' symptoms and immunologic function.

[Phenylhexyl isothiocyanate induces gene p15 demethylation by down-regulating DNA methyltransferases in Molt-4 cells].

This study is to investigate the effect of phenylhexyl isothiocyanate (PHI), which has been proved to be a novel histone deacetylase inhibitor (HDACi) recently, on gene p15 de novo expression in acute leukemia cell line Molt-4, and to further study its potential mechanism. Modified methylation specific PCR (MSP) was used to screen p15-M and p15-U mRNA. DNA methyltransferasel (DNMT1), 3A (DNMT3A), 3B (DNMT3B) and p15 mRNA were measured by RT-PCR. P15 protein was detected by Western blotting. Hypermethylation of gene p15 was reversed and activation transcription of gene p15 in Molt-4 was de novo after 5 days exposure to PHI in a concentration dependent manner. DNMT1 and DNMT3B were inhibited by exposure to PHI for 5 days (P < 0.05). Alteration of DNMT3A was not significant. It is showed that PHI could reverse hypermethylation of gene p15 and transcriptional activation of gene p15 is de novo by PHI. It may result from down-regulating DNA methyltransferases, DNMT1 and DNMT3B, or up-regulating the histone acetylation that allows chromatin unfolding and the accessibility of regulators for transcriptional activation in the p15 promoter.