ABSTRACT
Three previously undescribed seco-dammarane triterpenoid glycosides O-Q (1-3) along with two known compounds (4 and 5) were isolated and characterized from the leaves of Cyclocarya paliurus. Their structures were determined by comprehensive analysis of 1 D, 2 D NMR and HRESIMS data. Compounds 1-5 were evaluated for their inhibitory effects against human pancreatic tumor (ASPC-1), human gastric carcinoma (SNU5), liver hepatocellular carcinoma (HEPG-2) and human colon tumor (HCT116) cell lines. Among them cyclocarioside P (2) showed somewhat inhibitory activity towards those tumor cells.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Juglandaceae/chemistry , Triterpenes/chemistry , Triterpenes/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Cell Line, Tumor , Drug Screening Assays, Antitumor , Glycosides/chemistry , HCT116 Cells , Hep G2 Cells , Humans , Magnetic Resonance Spectroscopy , Molecular Structure , Pancreatic Neoplasms/drug therapy , Pancreatic Neoplasms/pathology , Plant Leaves/chemistry , DammaranesABSTRACT
Four new rarely occurred seco-dammarane triterpenoid glycosides (1-4) and four new dammarane triterpenoid glycosides (5-8), along with four known triterpenoids (9-12), were isolated from the 70% ethanol extract of the leaves of Cyclocarya paliurus (family Juglandaceae). Their structures were elucidated by extensive spectroscopic methods, including 1D/2D NMR and HRESIMS data, together with chemical analysis and DFT GIAO 13C NMR calculation. In bioassay, compounds 5-8 significantly increased glucose consumption in 3T3-L1 adipocytes, which could be the bioactive constituents for the anti-diabetes effect of the traditional usage of C. paliurus.
