ABSTRACT
The tea plant is a kind of ammonium-preferring crop, but the mechanism whereby ammonium (NH4 +) regulate its growth is not well understood. The current study focused on the effects of NH4 + on tea plants. Transcriptomic analysis was performed to investigate the early- and late-stage NH4 + deprivation and resupply in tea plants shoots. Through short- and long-term NH4 + deficiency, the dynamic response to NH4 + stress was investigated. The most significant effects of NH4 + deficiency were found to be on photosynthesis and gene ontology (GO) enrichment varied with the length of NH4 + deprivation. Enriched KEGG pathways were also different when NH4 + was resupplied at different concentrations which may indicate reasons for tolerance of high NH4 + concentration. Using weighted gene co-expression network analysis (WGCNA), modules related to significant tea components, tea polyphenols and free amino acids, were identified. Hence, NH4 + could be regarded as a signaling molecule with the response of catechins shown to be higher than that of amino acids. The current work represents a comprehensive transcriptomic analysis of plant responses to NH4 + and reveals many potential genes regulated by NH4 + in tea plants. Such findings may lead to improvements in nitrogen efficiency of tea plants.
ABSTRACT
As a preferred nitrogen form, ammonium (NH4 + ) transport via specific transporters is particularly important for the growth and development of tea plants (Camellia sinensis L.). However, our understanding of the functions of the AMT family in tea plants is limited. We identified and named 16 putative AMT genes according to phylogenetic analysis. All CsAMT genes were divided into three groups, distributed on 12 chromosomes with only one segmental duplication repetition. The CsAMT genes showed different expression levels in different organs, and most of them were expressed mainly in the apical buds and roots. Complementation analysis of yeast mutants showed that CsAMTs restored the uptake of NH4 + . This study provides insights into the genome-wide distribution and spatial expression of AMT genes in tea plants.
Subject(s)
Ammonium Compounds , Camellia sinensis , Ammonium Compounds/metabolism , Camellia sinensis/genetics , Camellia sinensis/metabolism , Gene Expression Regulation, Plant , Phylogeny , Plant Proteins/genetics , Plant Proteins/metabolism , Tea/metabolismABSTRACT
Vertically aligned carbon nanotube (VACNT) array/polymer composite has already been recognized as a promising candidate for advanced thermal pad in thermal management of high-power electronic devices. However, the thermal conductive performance of this composite was limited by the quality of CNTs arrays. In this study, pre-annealing treatment was used to purify CNT arrays and improve thermal conductive performance of VACNT arrays/silicone composite. The thermal conductivity of the composite was enhanced by 34.52% and the thermal interface resistance was also reduced by 65.94% at a pre-annealing temperature of 490 °C for 5 min. The annealing process could remove some amorphous carbon and open the tips of CNTs. As a result, the interfacial compatibility in composite between carbon nanotube and polymer matrix was improved. The cyclic compression and tension performance of VACNT/S160 composite was investigated for further application.
ABSTRACT
Laser speckle velocimetry (LSV) is presented to measure the velocities of nanoparticles in nanofluids and its feasibility is verified in this paper. An optical scattering model of a single nanoparticle is developed and numerical computations are done to simulate the formation of the speckles by the addition of the complex amplitudes of the scattering lights from multiple nanoparticles. Then relative experiments are done to form speckles when nanofluids are illuminated by a laser beam. The results of the experiments are in agreement with the numerical results, which verify the feasibility of utilizing LSV to measure the velocities of nanoparticles in nanofluids.
