Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
1.
J Proteome Res ; 6(1): 150-9, 2007 Jan.
Article in English | MEDLINE | ID: mdl-17203959

ABSTRACT

Pulmonary embolism (PE) is a common, potentially fatal disease and its diagnosis is challenging because clinical signs and symptoms are nonspecific. In this study, to investigate protein alterations of a rat PE model, total serum proteins collected at different time points were separated by two-dimensional electrophoresis (2-DE) and identified using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Bioinformatics analysis of 24 differentially expressed proteins showed that 20 had corresponding protein candidates in the database. According to their properties and obvious alterations after PE, changes of serum concentrations of Hp, Fn, DBP, RBP, and TTR were selected to be reidentified by western blot analysis. Semiquantitative RT-PCR showed DBP, RBP, and TTR to be down-regulated at mRNA levels in livers but not in lung tissues. The low serum concentrations of DBP, RBP, and TTR resulted in the up-regulation of 25(OH)D3, vitamin A, and FT4 (ligands of DBP, RBP, and TTR) after acute PE in rat models. The serum levels of Hp and Fn were detected in patients with DVT/PE and controls to explore their diagnostic prospects in acute PE because the mRNA levels of Hp and Fn were found to be up-regulated both in lung tissues and in livers after acute PE. Our data suggested that the concentration of serum Fn in controls was 79.42 +/- 31.57 microg/L, whereas that of PE/DVT patients was 554.43 +/- 136.18 microg/L (P < 0.001), and that the concentration of serum Hp in controls was 824.37 +/- 235.24 mg/L, whereas that of PE/DVT patients was 2063.48 +/- 425.38 mg/L (P < 0.001). The experimental PE rat model selected in this study was more similar to the clinical process than the other existing PE animal models, and the findings indicated instant changes of serum proteins within 48 h after acute PE. The exploration of these differentially expressed proteins or their combination with existent markers such as D-dimer may greatly improve the accuracy of the diagnosis of acute PE, but diagnostic tests are still needed to evaluate the sensitivity and specificity of these markers and also the number of false positives and false negatives.


Subject(s)
Biomarkers/metabolism , Blood Proteins/analysis , Mass Spectrometry/methods , Proteomics/methods , Pulmonary Embolism/blood , Acute Disease , Adult , Aged , Animals , Case-Control Studies , Female , Ferritins/blood , Ferritins/metabolism , Fibrin Fibrinogen Degradation Products/metabolism , Haptoglobins/metabolism , Humans , Male , Middle Aged , Rats
2.
J Health Popul Nutr ; 25(4): 488-94, 2007 Dec.
Article in English | MEDLINE | ID: mdl-18402193

ABSTRACT

The study was conducted to assess the nutritional status and levels of body mass index (BMI, kg/m2) and to evaluate the geographical distribution of male youths of the Han nationality in China. In total, 60,773 male youths, aged 18-20 years, of the Han nationality, were categorized into underweight, normal-weight, overweight, and obesity according to the international adult BMI cut-offs. Different levels of nutritional status and BMI of male youths of the Han nationality were compared among different areas. The mean BMI for the whole country was 20.6 in urban areas and 20.0 in rural areas. BMI increased from 20.1 among 18-year old youths to 20.5 among 20-year old youths. The prevalence of underweight among the male youths was 21.6%, while the prevalence of overweight and obesity were 4.6% and 0.6% respectively. For urban youths, the prevalence of underweight, overweight, and obesity were 21.0%, 6.8%, and 1.1% respectively, while these were, respectively, 21.9%, 3.3%, and 0.3% for rural youths. The nutritional status of the male youths in North-China was at the highest level (21.1) among the six areas, and the prevalence of underweight, overweight, and obesity were 14.3%, 9.1%, and 1.4% respectively. The highest prevalence of underweight was 29.8% in the North-West region, and the lowest prevalence of overweight was 2.2% in the South-Middle region, while the lowest prevalence of obesity was 0.2% in the South-West region. The nutritional status of the male youths was significantly different among different areas. Underweight was still prevalent in all male youth groups. Nonetheless, overweight was more prevalent among urban youths than among rural youths and was more prevalent in the North region than in the South region.


Subject(s)
Body Mass Index , Nutritional Status , Obesity/epidemiology , Overweight/epidemiology , Thinness/epidemiology , Adolescent , Adult , Body Weight/physiology , China/epidemiology , Demography , Humans , Male , Prevalence , Rural Population , Urban Population
3.
World J Gastroenterol ; 10(2): 231-3, 2004 Jan 15.
Article in English | MEDLINE | ID: mdl-14716829

ABSTRACT

AIM: To synthetically analyze and probe into the diagnosis of H pylori infection, we followed the principles of evidence-based medicine. METHODS: A total of 22 papers of prevalence survey and case-control studies were selected for studying about diadynamic methods. Using meta-analysis, we analyzed the different diadynamic methods of H pylori in China. RESULTS: Through meta-analysis, among the five diadynamic methods, the accuracy of polymerase chain reaction (PCR) was the highest (98.47%) and PCR was the most sensitive method (Sp: 99.03%). CONCLUSION: Among the five diadynamic methods, the accuracy of PCR is the highest and PCR is the most sensitive method to diagnose the infection of H pylori.


Subject(s)
Asian People , Helicobacter Infections/diagnosis , Helicobacter pylori/isolation & purification , Helicobacter Infections/ethnology , Helicobacter pylori/genetics , Humans , Polymerase Chain Reaction , Prevalence
4.
Zhonghua Liu Xing Bing Xue Za Zhi ; 24(1): 54-7, 2003 Jan.
Article in Chinese | MEDLINE | ID: mdl-12678965

ABSTRACT

OBJECTIVE: To understand and analyze the infection situation of Helicobacter pylori (H. pylori). METHODS: Extensively reviewing Chinese literature collecting the related with electronic documents in combination with manual retrieve and using Meta-analysis to do a quantitative analysis. RESULTS: Slight difference in the infection rate of H. pylori between men and women (95% CI: 0.0579-0.0963) was noticed. The infection rate of H. pylori in children whose parent was positive with infection of this bacteria was higher than that of children whose parent was negative (95% CI: 0.3378-0.5042). CONCLUSION: The infection rate of H. pylori showed gender difference with nature of family aggregation. Epidemiological studies of H. pylori was comprehensive and involved many aspects. Further investigation needs to be focused on infection rate and other risk factors.


Subject(s)
Family Health , Helicobacter Infections/epidemiology , Helicobacter pylori , Adolescent , Adult , Aged , Antibodies, Bacterial/blood , Child , Child, Preschool , China/epidemiology , Female , Helicobacter Infections/transmission , Helicobacter pylori/immunology , Humans , Infant , Male , Middle Aged , Prevalence , Risk Factors , Sex Factors
SELECTION OF CITATIONS
SEARCH DETAIL
...