Microplastic fragmentation by rotifers in aquatic ecosystems contributes to global nanoplastic pollution.

The role of aquatic organisms in the biological fragmentation of microplastics and their contribution to global nanoplastic pollution are poorly understood. Here we present a biological fragmentation pathway that generates nanoplastics during the ingestion of microplastics by rotifers, a commonly found and globally distributed surface water zooplankton relevant for nutrient recycling. Both marine and freshwater rotifers could rapidly grind polystyrene, polyethylene and photo-aged microplastics, thus releasing smaller particulates during ingestion. Nanoindentation studies of the trophi of the rotifer chitinous mastax revealed a Young's modulus of 1.46 GPa, which was higher than the 0.79 GPa for polystyrene microparticles, suggesting a fragmentation mechanism through grinding the edges of microplastics. Marine and freshwater rotifers generated over 3.48 × 105 and 3.66 × 105 submicrometre particles per rotifer in a day, respectively, from photo-aged microplastics. Our data suggest the ubiquitous occurrence of microplastic fragmentation by different rotifer species in natural aquatic environments of both primary and secondary microplastics of various polymer compositions and provide previously unidentified insights into the fate of microplastics and the source of nanoplastics in global surface waters.

Mechanistic Understanding toward the Maternal Transfer of Nanoplastics in Daphnia magna.

Nanoplastics induce transgenerational toxicity to aquatic organisms, but the specific pathways for the maternal transfer of nanoplastics remain unclear. Herein, laser scanning confocal microscopy (LSCM) observations identified the specific pathways on the maternal transfer of polystyrene (PS) nanoplastics (25 nm) in Daphnia magna. In vivo and in vitro experiments showed that PS nanoplastics could enter the brood chamber through its opening and then be internalized to eggs and embryos using LSCM imaging (pathway I). In addition, PS nanoplastics were observed in the oocytes of the ovary, demonstrating gut-ovary-oocyte transfer (pathway II). Furthermore, label-free hyperspectral imaging was used to detect the distribution of nanoplastics in the embryos and ovary of Daphnia, again confirming the maternal transfer of nanoplastics through the two pathways mentioned above. The contribution from pathway I (88%) was much higher than pathway II (12%) based on nanoflow cytometry quantification. In addition, maternal transfer in Daphnia depended on the particle size of PS nanoplastics, as demonstrated by using LSCM and hyperspectral imaging. Unlike 25 nm nanoplastics, 50 nm PS nanoplastics could enter the brood chamber and the eggs/embryos (pathway I), but were not detected in the ovary (pathway II); 100 nm PS nanoplastics were difficult to be internalized by eggs/embryos and could not enter the ovary either. These findings provide insight into the maternal transfer mechanisms of nanoplastics in Daphnia, and are critical for better understanding the transgenerational toxicity of aquatic organisms.

The effects of salinity changes on anammox performance: The response rule and tolerance mechanism.

Some wastewaters contain high concentrations of ammonia coexisting with large amounts of salt, which might negatively affect the anaerobic ammonium oxidation (anammox) process. In this study, the performance of the anammox process under different saline conditions was investigated using an upflow anaerobic sludge bed-anammox system. After long-term operating for 275 days, the results indicated that the nitrogen removal efficiency remained high under the 0-40 g NaCl/L, and low salinity (15 g NaCl/L) substantially promoted specific anammox activity. Affected by the saline environment, the appearance, color, and shape of sludge notably changed, and the amount of extracellular polymeric substances gradually increased with increasing salinity, which might be one of the reasons for the strong salt tolerance of the system. Chloroflexi and Planctomycetes were the dominant strains under long-term salinity, and Brocadiaceae_g_ unclassified exhibited halophilic characteristics. The redundancy analysis results showed that the concentration of influent NH4 + -N and salinity were the main environmental factors affecting the microbial community of the system. PRACTITIONER POINTS: Provides data to support the maximum value for salinity wastewater treatment with anammox processes' tolerance of 40 g NaCl/L. EPS changes may be responsible for the response to salinity challenges and provide direction for high salinity wastewater treatment. Brocadiaceae_g_ unclassified exhibited a halophilic quality. And it can be focused on to improve treatment efficiency.

Precise Tailoring of Polyester Bottlebrush Amphiphiles toward Eco-Friendly Photonic Pigments via Interfacial Self-Assembly.

Biodegradable photonic microspheres with structural colors are promising substitutes to polluting microbeads and toxic dyes. Here, amphiphilic polyester-block-poly(ethylene glycol) bottlebrush block copolymers (BBCPs) with polylactic acid or poly(ϵ-caprolactone) as the hydrophobic block are synthesized and used to fabricate eco-friendly photonic pigments. Molecular parameters of BBCPs, including rigidity and symmetry, are precisely tailored by variation of side chain lengths, which enables effective manipulation of interfacial tension (γ). Organized spontaneous emulsion mechanism is enabled only when γ falls in a suitable range (10.6-14.3 mN m-1 ), producing ordered water-in-oil-in-water multiple emulsions and ordered porous structures. Consequently, highly saturated and tunable structural colors are observed due to coherent light scattering from the porous structures. Such photonic materials are nontoxic as confirmed by careful safety tests using aquatic model organisms.

Heteroaggregation between graphene oxide and titanium dioxide particles of different shapes in aqueous phase.

Graphene oxide (GO) inevitably interacts with engineered and/or natural nanoparticles (NPs) after releasing into aquatic environments. It is observed that GO could form heteroaggregates with differently shaped TiO2 NPS at pHs 4.0 and 7.0 rather than pH 10.0. Optimal pHs for the maximal heteroaggregation were around 6.63 and 4.92 for TiO2 spheres and fibers, respectively, which was dominated by electrostatic attraction. The optimal concentration ratio for TiO2 spheres was 10 times higher than TiO2 fibers, due to cross-linking configuration and stronger van der Waals force of TiO2 fibers with GO. Pre-homoaggregation of TiO2 NPs could promote subsequent heteroaggregation while the promotion was negligible at high ionic strength (e.g., > critical coagulant concentrations), and a longer pre-homoaggregation time showed a stronger promotion (750 s > 150 s > 0 s). Natural organic matter (NOM) obviously inhibited GO-TiO2 heteroaggregation at pH 4.0 compared with pH 7.0 due to stronger steric hindrance. Moreover, strong disturbance (e.g., sonication) could break down the heteroaggregates, and the disaggregation and re-aggregation processes were shape-independent. This work provides new insights into the role of NPs shapes and homoaggregation on the interaction of GO with both engineered and natural particles/minerals, and the fate of GO in aquatic environments.

Improvement of Biomineralization of Sporosarcina pasteurii as Biocementing Material for Concrete Repair by Atmospheric and Room Temperature Plasma Mutagenesis and Response Surface Methodology.

Microbially induced calcium carbonate precipitation (MICP) has recently become an intelligent and environmentally friendly method for repairing cracks in concrete. To improve on this ability of microbial materials concrete repair, we applied random mutagenesis and optimization of mineralization conditions to improve the quantity and crystal form of microbially precipitated calcium carbonate. Sporosarcina pasteurii ATCC 11859 was used as the starting strain to obtain the mutant with high urease activity by atmospheric and room temperature plasma (ARTP) mutagenesis. Next, we investigated the optimal biomineralization conditions and precipitation crystal form using Plackett-Burman experimental design and response surface methodology (RSM). Biomineralization with 0.73 mol/l calcium chloride, 45 g/l urea, reaction temperature of 45°C, and reaction time of 22 h, significantly increased the amount of precipitated calcium carbonate, which was deposited in the form of calcite crystals. Finally, the repair of concrete using the optimized biomineralization process was evaluated. A comparison of water absorption and adhesion of concrete specimens before and after repairs showed that concrete cracks and surface defects could be efficiently repaired. This study provides a new method to engineer biocementing material for concrete repair.

Use of thermally modified waste concrete powder for removal of Pb (II) from wastewater: Effects and mechanism.

Exploring effective uses of waste concrete powder (WCP), produced from recycling of construction & demolition waste is beneficial to the environment and sustainable development. In this study, WCP was first treated thermally to enhance the ability to remove Pb (II) from aqueous solutions. The experimental results revealed that the thermal treatment could enhance adsorption capacity due to modification of calcium bonding and pore structure of WCP. Preparation parameters such as temperature, particle size, and water-cement ratio were investigated to obtain the optimal operational conditions. Batch adsorption experiments were performed to explore influence factors of pH (1.00-6.00), ionic strength (0.05-2 mol/L), dosage (2-50 g/L), and temperature (25-45 °C). The pseudo-second-order kinetics model could adequately describe the adsorption process, and the Langmuir model was capable to predict the isotherm data well in the low concentration region (C0 < 500 mg/L). The maximum uptake capacity for Pb (II) calculated by Langmuir model at 25, 35 and 45 °C were 46.02, 38.58 and 30.01 mg/g respectively, and the removal rate of Pb (II) was 92.96% at a dosage of 50 g/L (C0 = 1000 mg/L). Precipitation, ion exchange, and surface complexation were identified to be the main mechanisms of Pb (II) adsorption through microscopic investigation by SEM-EDX, XRD, FTIR, XPS, and BET inspections. The study confirms that the WCP after thermal modification, can be selected as a promising adsorbent for the high performance and eco-friendliness.

Reverberation reduction based on multi-ping association in a moving target scenario.

Conventional reverberation reduction methods are conducted with single-ping data and may fail in a low signal-to-reverberation ratio (SRR) environment. To improve the performance of reverberation reduction, multi-ping data are fully considered in this paper. The reverberation can be treated as a combination of the steady component of reverberation and reverberation fluctuations, and then an alternating direction multiplier method is proposed to reduce the steady component of the reverberation. By exploiting the evolution of the target location along multiple pings, the reverberation fluctuation is reduced by the probabilistic data association method. The proposed method was verified by the field data, and the results show that compared with the accelerated proximal gradient method, the sparse coefficient is improved by a factor of 1.23, and the signal excess is improved by an average value of 2.0 dB. In addition, the performance of the proposed method is found to be closely related to the signal-to-reverberation-fluctuation ratio rather than only the SRR.

Localization of Two Sound Sources Based on Compressed Matched Field Processing with a Short Hydrophone Array in the Deep Ocean.

Passive multiple sound source localization is a challenging problem in underwater acoustics, especially for a short hydrophone array in the deep ocean. Several attempts have been made to solve this problem by applying compressive sensing (CS) techniques. In this study, one greedy algorithm in CS theory combined with a spatial filter was developed and applied to a two-source localization scenario in the deep ocean. This method facilitates localization by utilizing the greedy algorithm with a spatial filter at several iterative loops. The simulated and experimental data suggest that the proposed method provides a certain localization performance improvement over the use of the Bartlett processor and the greedy algorithm without a spatial filter. Additionally, the effects on the source localization caused by factors such as the array aperture, number of hydrophones or snapshots, and signal-to-noise ratio (SNR) are demonstrated.