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Yi Chuan ; 27(6): 882-6, 2005 Nov.
Article in Chinese | MEDLINE | ID: mdl-16378933

ABSTRACT

The genetic variation in 5' - upstream (Hinf I -RFLP)and the second intron (Hinf I *-RFLP, Hae III-RFLP)of heart fatty acid-binding protein(H-FABP)gene were detected with PCR-RFLP in 286 pigs including Mashen, Shanxi white pig and their crossbred populations. The results showed as follows: (1)Mashen presented only DD genotype while other populations varied,and Mashen crossbred populations had only 2 genotypes(DD, Dd) at the Hae III-RFLP site; (2)At the Hinf I -RFLP site of the 5' -upstream region, the crossbred population of Shanxi white pig and Duroc presented only HH genotype while other populations varied. Frequency of h allele in Mashen was 0.9727. (3)At the Hinf I *-RFLP site of the second intron, only Mashen presented 2 genotypes (BB, Bb), and frequency of B allele was 0.9667. (4)At the Hae III-RFLP and Hinf I *-RFLP sites, all populations were in Hardy-weinberg equilibrium.


Subject(s)
Fatty Acid-Binding Proteins/genetics , Polymerase Chain Reaction/methods , Polymorphism, Genetic , Swine/genetics , Alleles , Animals , Crosses, Genetic , Female , Gene Frequency , Genotype , Linkage Disequilibrium , Male , Polymorphism, Restriction Fragment Length , Swine/classification
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