ABSTRACT
Adjuvant radiotherapy has become an important part of the standard treatment for breast cancer. Compared with the traditional postoperative radiotherapy, neoadjuvant radiotherapy has the theoretical advantages of more accurate target delineation, optimization of radiation strategy for breast reconstruction, increase of breast conservation surgery with tumor down-staging and avoiding breast surgery by improving pathological complete response rate, which have been confirmed by recent clinical research. Prospective studies are still needed for the optimal target delineation, dose fractionation, radiation-surgery interval, and combination with systemic therapies, aiming to provide the optimal treatment option for patients with early breast cancer.
ABSTRACT
Bone marrow-derived mesenchymal stem cells (BMSCs) are easy to collect and culture, and it is identified that it has multi-directional differentiation potential, moreover it has low immunogenicity, hence it can be used as an allogeneic cell source for skin wound healing. Hydrogel has been widely used in skin wound healing own to it is able to mimic the 3D microenvironment of cells, which supports cell proliferation, migration and secretion. In this study, we created a novel biocompatible thermo-sensitive hydrogel to carry BMSCs for full-thickness skin wound healing. The thermo-sensitive hydrogel loaded with BMSCs can fast achieve sol-gel transition after implanting to the wound. Histological results confirmed that hydrogel-BMSCs combination group showed significant promotion of wound closure, epithelial cells' proliferation and re-epithelialization, and reduced inflammatory responses in the wounds and in the tissues surrounding the wounds. The combination therapy also can promote collagen deposition, TGF-ß1 and bFGF secretion and tissue remodeling. The present study provides a promising strategy for the clinical treatment of skin wounds.
