ABSTRACT
Objective To study the clinical efficacy and adverse reactions of different blue-light and expo-sure patterns for the treatment of neonatal hyperbilirubinemia.Methods 127 neonates with hyperbilirubinemia were divided into cold-light illuminator blue-light irradiation group (group A, n=32), cold-light illuminator blue-light group intermittent irradiation group (group B, n=32), traditional blue-light irradiation group (group C, n=31) and traditional blue-light intermittent irradiation group (group D, n=32).The treatment course of the four groups was all 72 hours.The level of serum total bilirubin ( TBIL) , direct bilirubin ( DBIL) and auditory brainstem response before and after treatment in the four groups were respectively observed and compared.The adverse reactions associated with treatment were recorded and compared among the four groups.After 9 months of follow-up, the audi-tory brainstem response ( ABR) was respectively tested at 3 days, 3 months and 9 months after the treatment.Results The level of TBIL in the four groups after treatment were significantly decreased compared with that before treatment (p0.05).The ABR in the four groups returned to the nor-mal after 9 months of treatment.Conclusion The therapy of the cold-light illuminator blue-light irradiation has significant clinical efficacy in the treatment of neonatal hyperbilirubinemia.It can effectively reduce the hearing im-pairment of neonates, and the incidence of adverse reaction and total effective rates are equivalent in therapeutic pat-terns of intermittent irradiation and continuous irradiation.
ABSTRACT
The derivatization towards the hydroxyl groups of steroid environmental endocrine disrupting chemicals,such as estrone(E1),17β-estradiol(E2),estriol(E3) and 17α-ethynylestradoil( EE2),with A,O-bis (trimethylsilyl) trifluoroacetamide ( BSTFA ) as derivatization reagent followed by gas chromatography-mass spectrometry(GC-MS) was studied. The effects of the amount of BSTFA,derivatization temperature and time on the derivatization of target compounds,as well as the stability of steroid derivatives,calibration curves,instrumental quantification limits were investigated. The cleavage mechanism of steroid derivatives characteristic fragment ions was also interpreted. The results showed that better derivatization results were obtained when the amount of BSTFA was 25 μL for 100 μL steroids standard mix solution at 0.01 g/L;it is unnecessary to heating in the derivatization,better results were obtained when the steroids were derivatized with BSTFA at room temperature (20℃) for 10 min. The derivatives of target compounds were stable because their relative response factors(RRFs) had no observable change when deposited at -20℃ for 48 h. Under the optimal conditions,good linear correlation was obtained and the instrumental quantification limit was 0. 3μg/L for E1 and E2,while it was 5μg/L for EE2 and E3.
