Research progress on relationship between N6-adenylate methylation modification and cell transformation and associated mechanism / 环境与职业医学

As a reversible and dynamic epigenetic marker, N6-adenylate methylation (m6A) modification is the most common mRNA modification in eukaryotes. This paper briefly described how m6A can influence RNA splicing, stability, and translation after transcription, and then participate in a variety of signaling pathways and biological and pathological processes, regulating cell proliferation, apoptosis, epithelial mesenchymal transformation (EMT) processes, and tumor invasion and metastasis. In addition, according to current studies, m6A methyltransferases (writers) are believed to promote EMT and tumor development, and readers and erasers both promote and inhibit EMT in different research objects. In this review, we summarized the mechanism of m6A modification and its role in cell transformation, and pointed out the direction of disease treatment.

Modification of m6 A in human embryonic lung fibroblasts induced by neodymium oxide exposure and its mechanism / 环境与职业医学

Background Occupational and environmental particulate matter may cause fibrosis, accompanied by RNA m6A modification changes. Neodymium oxide (Nd2O3) can cause mouse lung fibrosis, which contains a large number of fibroblasts. Objective To investigate m6A modification of tumor necrosis factor receptor-associated protein 6/nuclear factor-κB (TRAF6/NF-κB) signaling pathway in fibrosis of human embryonic lung fibroblasts induced by Nd2O3, and identify the key m6A modification sites of TRAF6. Methods Designed concentrations of Nd2O3 (0, 1.563, 3.125, 6.25, 12.5, 25, 50, 100, and 200 mg∙L−1) were infected with HELF cells for 24 and 48 h, and cell viability was detected to determine exposure time and dose. Measurements included indicators of fibrosis [hydroxyproline (HYP) and transforming growth factor-β1 (TGF-β1)], m6A methylation level, methyltransferases (METTL3 and METTL14), demethylases (FTO and ALKBH5), reading proteins (YTHDC2 and YTHDF2), fibrosis-associated genes (collagen-І, vimentin, and α-SMA), and proteins related to signaling pathway (TRAF6, NFKB1, P65, and P-P65). The enrichment of m6A in TRAF6 mRNA was measured by methylated RNA immunoprecipitation-quantitative real-time PCR (MeRIP-qPCR). Results The results of cell viability indicated that 6.25, 12.5, 25 mg∙L−1 Nd2O3 and 48 h exposure time were used for subsequent experiments. After 48 h exposure, compared with the control group, the HYP level in the 25 mg∙L−1 Nd2O3 group was increased, and the levels of TGF-β1 in the 6.25, 12.5, and 25 mg∙L−1 Nd2O3 groups were increased (P<0.05); the overall m6A methylation levels of HELF cells in the 12.5 and 25 mg∙L−1 Nd2O3 groups were increased (P<0.05). At mRNA level, compared with the control group, the mRNA expression levels of methyltransferases METTL3 and METTL14 (6.25, 12.5, and 25 mg∙L−1 Nd2O3) were increased (P<0.05); the mRNA expression level of reading protein YTHDF2 (6.25, 12.5, and 25 mg∙L−1 Nd2O3) was increased (P<0.05), while the mRNA expression level of YTHDC2 (25 mg∙L−1 Nd2O3) was decreased (P<0.05); the mRNA expression levels of demethylases FTO (12.5 and 25 mg∙L−1 Nd2O3) and ALKBH5 (25 mg∙L−1 Nd2O3) were decreased (P<0.05); the mRNA expression levels of fibrosis-related genes vimentin, α-SMA, and collagen-Ⅰ (6.25, 12.5, and 25 mg∙L−1 Nd2O3) were increased (P<0.05); the mRNA expression levels of pathway-related genes TRAF6 (25 mg∙L−1 Nd2O3) and NFKB1 (12.5 and 25 mg∙L−1 Nd2O3) were increased (P<0.05). At protein level, compared with the control group, the expression levels of methyltransferases METTL3 (25 mg∙L−1 Nd2O3) and METTL14 (12.5 and 25 mg∙L−1 Nd2O3) were increased (P<0.05); the expression level of reading protein YTHDF2 (12.5 and 25 mg∙L−1 Nd2O3) was increased, while the expression level of YTHDC2 (25 mg∙L−1 Nd2O3) was decreased (P<0.05); the expression level of demethylase FTO (25 mg∙L−1 Nd2O3) was decreased (P<0.05); the expression level of fibrosis-associated protein vimentin was increased at 25 mg∙L−1 Nd2O3, and the expression levels of α-SMA and collagen-Ⅰ were increased at 12.5 and 25 mg∙L−1 Nd2O3 (P<0.05); the expression levels of TRAF6 and P-P65 were increased at 25 mg∙L−1 Nd2O3 (P<0.05). The MeRIP-qPCR results showed that compared with the control group, the concentrations of m6A in all Nd2O3 groups were significantly increased (P<0.05). Conclusions Upon exposure of HELF cells to Nd2O3, the alterations in fibrosis-related indexes increase the expression of some m6A methylases and decrease the expression of demethylases, thereby increasing the m6A methylase level, and may promote the progression of fibrosis by activating the TRAF6/NF-κB signaling pathway.

Changes of m6A modification in arsenic-induced oxidative stress of human embryonic lung fibroblasts / 环境与职业医学

Background Arsenic can be toxic to human by triggering oxidative stress, which is companied by epigenetic modifications. Objective To investigate the modification of N6-methyladenosine (m6A) in human embryonic lung fibroblasts (HELF) during oxidative stress induced by sodium arsenite (NaAsO2). Methods HELF cells were treated by designed concentrations of NaAsO2 (0, 2.5, 5, 10, and 20 μmol·L−1) for 48 h. Cell viability was detected by 3-(4,5-dimethylthia zol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfopheny)-2H-tetrazolium (MTS) method; the activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) as well as the content of malondialdehyde (MDA) were detected with corresponding kits; the level of m6A methylation in total RNA was detected by enzyme-linked immunosorbent assay; the mRNA expressions of m6A modified enzymes were detected by real-time fluorescence quantitative PCR, including methyltransferase-like 3 (METTL3), methyltransferase-like 14 (METTL14), Wilms' tumor 1-associated protein (WTAP), fat mass and obesity-associated protein (FTO), alkB family of Fe(II)/α-ketoglutarate-dependent dioxygenases 5 (ALKBH5), YTH domain containing protein 2 (YTHDC2), YTH domain family protein 2 (YTHDF2), and YTH domain family protein 3 (YTHDF3); the protein expressions of METTL3, FTO, YTHDC2, YTHDF3, and nuclear factor erythroid 2-related factor 2 (NRF2) were detected by Western blotting. The enrichment of m6A in NRF2 mRNA was detected by RNA methylated immunoprecipitation combined with real-time fluorescence quantitative PCR (MeRIP-qPCR). Results After the 0, 2.5, 5, 10, and 20 μmol·L−1 NaAsO2 treatment, the MTS results showed that compared with the control group, the cell viability of the 20 μmol·L−1 group decreased to 84% (P<0.05). The colorimetry results showed that compared with the control group, the activities of T-SOD in the 10 and 20 μmol·L−1 groups decreased (P<0.05); the activities of GSH-Px in the 2.5 and 10 μmol·L−1 groups decreased (P<0.05); the contents of MDA in the 10 and 20 μmol·L−1 groups increased. The results of enzyme-linked immunosorbent assay showed that the overall m6A methylation levels in the 0, 2.5, 5, 10, and 20 μmol·L−1 groups were (0.193 ± 0.023)%, (0.247 ± 0.021)%, (0.253 ± 0.006)%, (0.233 ± 0.006)%, and (0.262 ± 0.010)%, respectively, and compared with the control group, the m6A methylation levels in all the NaAsO2 treated groups increased (P<0.05). The real-time fluorescence quantitative PCR results showed that compared with the control group, the mRNA relative expression level of METTL3 decreased in the 2.5, 10, and 20 μmol·L−1 groups (P<0.05); the mRNA relative expression level of FTO decreased in the 20 μmol·L−1 group; the mRNA relative expression level of YTHDC2 increased in the 10 and 20 μmol·L−1 groups (P<0.05); the mRNA relative expression level of YTHDF3 increased in the 2.5, 10, and 20 μmol·L−1 groups (P<0.05). The Western blotting results showed that compared with the control group, the relative protein expression of METTL3 decreased in the 10 and 20 μmol·L−1 groups; the relative protein expression of FTO decreased in the 5 and 20 μmol·L−1 groups; the relative protein expression of YTHDC2 decreased in the 20 μmol·L−1 group (P<0.05); the relative nuclear protein expression of NRF2 decreased in the 10 and 20 μmol·L−1 groups (P<0.05). The MeRIP-qPCR results showed that m6A enrichment was significantly increased in the 20 μmol·L−1 NaAsO2 exposure group compared with the control group (P<0.05). After over-expression of FTO, the mRNA and protein relative expression levels of FTO and the relative expression level of nuclear protein of NRF2 in the FTO group were higher than those in the control group (P<0.05); the mRNA and protein relative expression levels of FTO in the NaAsO2 + FTO group and the nuclear protein expression level of NRF2 were higher than those in the NaAsO2 group (P<0.05). Conclusion In the process of oxidative stress induced by NaAsO2, m6A methylation level, m6A modified enzymes, m6A modification of NRF2 mRNA, and NRF2 expression could change in HELF cells.

Determination of samarium oxide and lanthanum oxide in the air of workplace by inductively coupled plasmamass spectrometry / 中华劳动卫生职业病杂志

Objective@#To establish a method for the determination ofsamarium oxide and lanthanum oxide by inductively coupled plasmamass spectrometryin the air of workplace.@*Methods@#Samarium, lanthanum and their compounds in the air of workplace were collected through microporous filter. The samples were digested by nitricacid and perhydrol (V/V=4∶1) and detected by inductively coupled plasmamass spectrometry.@*Results@#The linear range ofsamarium oxide and lanthanum oxide was 0-50.00 μg/L, Sm2O3: y=0.0119x, r=0.9999; La2O3: y=0.0617x, r=0.9998. The detection limits were less than 0.1 μg/L, and the minimum detection concentration were less than 1.52×10-5 mg/m3. The sampling efficiency were 100%, the recovery rates were 95.70%-102.01%, and the precision were 0.78%-1.58%.@*Conclusion@#The indicators established in this study are conformed with the requirements of Chinese Occupational Standars of GBZ/T 210.4-2008, "The Guidelines for the Development of Occupational Hygiene StandarsMehods Part 4: Determination of Chemical Substances in the Air of Workplace".

Resuscitation acupuncture for thalamic pain:a randomized controlled trial / 中国针灸

<p><b>OBJECTIVE</b>To compare the effects between resuscitation acupuncture and pregabalin for thalamic pain and their impacts on plasma P substance (SP) and β-endorphin (β-EP).</p><p><b>METHODS</b>Sixty-four patients were randomly assigned into an acupuncture group and a western medication group, 32 cases in each one. Based on conventional western methods, pregabalin capsule was used orally in the western medication group, 75 mg a time,twice a day; resuscitation acupuncture was applied in the acupuncture group. The main acupoints were Shuigou (GV 26), Neiguan (PC 6), Sanyinjiao (SP 6). Patients with upper limb pain were attached affected Jiquan (HT 1), Chize (LU 5), and Hegu (LI 4); lower limb pain, affected Weizhong (BL 40), Zusanli (ST 36); hea-dache, bilateral Fengchi (GB 20), Wangu (GB 12), and Yifeng (TE 17), twice a day. Treatment was given 6 d a week for 8 weeks in the two groups. The changes of simplified McGill pain questionnaire (SF-MPQ), plasma SP and β-EP were observed before and after 4-week, 8-week treatment, as well as at follow-up, namely, 3 months after treatment. Also, clinical effects were evaluated.</p><p><b>RESULTS</b>The total effective rate of the acupuncture group was 50.0% (16/32) after 4-week treatment, which was similar to 46.9% (15/32) in the western medication group (>0.05). While after 8-week treatment and at follow-up, the total effective rates of the acupuncture group were 90.6% (29/32) and 84.4% (27/32), which were better than 65.6% (21/32) and 40.6% (13/32) of the western medication group correspondingly (both<0.05). After 4-week, 8-week treatment and at follow-up, the pain scores of the acupuncture group were lower than that before treatment (all<0.05). After 4-week and 8-week treatment, the pain scores of the western medication group were lower than that before treatment (both<0.05). After 8-week treatment and at follow-up, the pain scores of the acupuncture group were superior to thoseof the western medication group (both<0.05). After 4-week and 8-week treatment,the contents of plasma SP reduced compared with those before treatment in the two groups (all<0.05), and plasma β-EP increased (all<0.05). After 8-week treatment, SP content of the acupuncture group was apparently lower than that of the western medication group (<0.05), and β-EP increased more obviously (<0.05).</p><p><b>CONCLUSIONS</b>Resuscitation acupuncture can effectively relieve the symptoms of thalamic pain with stable and long-term effect, and it is better than pregabalin. Meanwhile, the acupuncture can increase β-EP and reduce SP.</p>

The Textual Research of LEI Feng's Life Story / 浙江中医药大学学报

[Objective]To study doctor LEI Feng's life story from Zhejiang Province in late Qing Dynasty from the point of view of the social history. [Methods]With the research methods of history and philology, collect relevant historical materials, to study LEI Feng 's life story from the view of social history. [Results]LEI Feng was born in the nineteenth year of the Daoguang Emperor of the Qing Dynasty(1839), died in the fourteenth year of the Guangxu Emperor of the Qing Dynasty(1888), at the age of 49. LEI Feng had a daughter named GONG Yuyun(about 1870-1899), a son named LEI Dazheng(?-1899), two students named ChENG Xi and JIANG Cheng, LEI Feng's father named LEI Huanran(?-about 1862), LEI Huanran's medical teacher was CHENG Zhitian. [Conclusion]LEI's medical family in Quzhou city of Zhejiang Province inherited from father to son and teacher to student, they both had their own medical literatures, the academic origin of LEI's medical family was Xin'an medicine;though LEI Feng called himself an ordinary person, he was good at writing poem, calligraphy and painting even music, he played a special role in traditional Chinese medicine 's social history.

A new textual criticism on block-printed editionin thedynasty / 中国针灸

It has always been considered that() printed byis the earliest version. In comparative study, it is found that there are two versions in thedynasty, one is the original, stored in the Library of China Academy of Sciences and another one is the revision, stored in the Library of Tianjin University of TCM, Zhejiang Provincial Library and the Library of China Academy of Chinese Medical Sciences. The last item, Chengjiang (CV 24) in thein volume 7 is included in the revised edition, but not covered in the original one, which is the difference between the two versions. Both of the versions were in thedynasty, rather than the block-printed one in thedynasty.

Discussion on different methods of painless needle insertion based on positions / 中国针灸

Based on multiple painless needle insertion skills in clinic combined with own experience, the authors propose the opinion that different painless needle insertion methods need to be used according to the positions where acupoints are. The appropriate needle insertion mean must be selected in accord with the anatomical characteristic of acupoint position, namely, tapping insertion is applied in scalp part, skin-pinching up needle insertion is used in forehead and superficial nerve trunk, fingernail-pressure needle insertion in ocular region and positions where large blood vessels distribute, flying acupuncture insertion in face and finger tip, vibrating needle in occipitonuchal region, quivering insertion in abdomen, quick pricking by single hand in the four limbs and back, and chop acupuncture in palm and pelma. The insertion methods are systematically discussed in terms of preparation, acupoint position, manipulation and attention, etc, so that to provide the reference for painless needle insertion.

"""Activating Brain and Regaining Consciousness"" Acupuncture Method Combined with Surrounding Needling Acupuncture on Shoulder Joint in Treatment of Periarthritis of Shoulder" / 世界科学技术-中医药现代化

This study was aimed to observe curative effect of periarthritis of shoulder with activating brain and re-gaining consciousness acupuncture method combined with surrounding needling acupuncture on shoulder joint. A to-tal of 120 patients were randomly divided into the treatment group (60 cases) and the control group (60 cases) on the basis of routine internal medicine treatment and rehabilitation exercises. Cases in the control group received conven-tional acupuncture treatment. And cases in the treatment group received activating brain and regaining conscious-ness acupuncture method combined with surrounding needling acupuncture on shoulder joint. The treatment course was 28 days. And then, the curative effect evaluation was conducted. The results showed that the effective rate was 73.33% in the control group and 93.34% in the treatment group. There was statistical significance on effective rate between both groups(P< 0.05). It was concluded that the activating brain and regaining consciousness acupuncture method combined with surrounding needling acupuncture on shoulder joint had significant curative effects for peri-arthritis of shoulder. This treatment method is worthy of further popularization and application.

The effects of electro-acupuncturing DU26 (renzhong) on motor cortical excitability and neurofunction after focal cerebral ischemia injury in rats.

OBJECTIVE: To study the effects of electro-acupuncturing DU26 (Renzhong) on cortical excitability and neurofunction after focal cerebral ischemia injury. METHOD: 30 male Wistar rats were divided randomly into control group, MCAo group and MCAo+DU26ES group, 10 in each group. Middle Cerebral Artery occlusion models (MCAo) were created in accordance with Zea Longa method and modified method. DC continuous square-wave electric pulse stimuli (2Hz, 3mA) which was given between DU26 and non-acupoint area in the left shoulder, was applied to MCAo+DU26ES group 10 minutes every time, twice a day, while the control group and MCAo group received no treatment. 3 days later transcranial electrical stimulation motor evoked potential (MEP) was recorded, the onset latency and peak-to-peak amplitude were analyzed and the neurological deficits were evaluated. The effects of electro-acupuncturing DU26 (Renzhong) and MCAo injury on these parameters were analyzed. RESULTS: Given the isointensity suprathreshold stimulus, the latency on the affected side in MCAo group was significantly longer than that in control group (P<0.05), the amplitudes on both sides were significantly reduced (P<0.05, P<0.05); the latencies on both sides in MCAo+DU26ES group tended to become shorter and the amplitudes on both sides tended to increase compared with MCAo group; there were no significant differences on latency and amplitude between the healthy side and the affected side in all groups (P>0.05). The neuro-scores of rats receiving electro-acupuncturing DU26 (Renzhong) were significantly higher than those in MCAo group (P<0.05). CONCLUSION: Electro-acupuncturing DU26 (Renzhong) can improve the motor cortical excitability, and facilitate the motor function recovery after MCAo injury.

Danqi Piantang Jiaonang (DJ), a traditional Chinese medicine, in poststroke recovery.

BACKGROUND AND PURPOSE: Stroke is a leading cause of death and disability worldwide. Despite improvements in acute stroke treatment, many patients only make a partial or poor recovery. Therefore, there is a need for treatments that would further improve outcome. Danqi Piantang Jiaonang (DJ; NeuroAid), a traditional Chinese medicine widely used in China to improve recovery after stroke, has been compared with another traditional Chinese medicine in 2 unpublished randomized clinical trials. The results of these studies were pooled and reanalyzed to assess efficacy and safety. METHODS: Six hundred five subjects were randomized in 2 randomized double-blinded, controlled trials to receive either DJ or Buchang Naoxintong Jiaonang. Subjects were treated for 1 month. Inclusion criteria were: (1) patients with recent (from 10 days to 6 months) ischemic stroke; (2) patients satisfying Western diagnostic standards for stroke and traditional Chinese medicine standards for diagnosis of apoplexy; and (3) Diagnostic Therapeutic Effects of Apoplexy score >/=10. RESULTS: The functional outcome, measured by the Comprehensive Function Score component of the Diagnostic Therapeutic Effects of Apoplexy scale, showed a statistically significant superiority of DJ over the control treatment group (relative risk, 2.4; 95% CI, 1.28 to 4.51; P=0.007). Tolerance was excellent in both groups. CONCLUSIONS: The pooled analysis of 2 unpublished trials of DJ, a traditional Chinese medicine currently approved in China to improve neurological recovery after stroke, shows good tolerability and superiority of DJ over another traditional Chinese medicine also approved for stroke. A large double-blind randomized clinical trial is required to further assess the safety and efficacy of DJ.

The Effect of "Xingnao Kaiqiao" Acupuncture on the Expression of Peroxiredoxin VI in the Ischemin Cortex of Rats / 医学研究杂志

Objective To investigate and observe the expression of Peroxiredoxin Ⅵ in iechemia cortex of rats and the intervention effect of Xingnao Kaiqiao Acupuncture(XKA) therapy. Methods Through western blotting,we detected the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group , comparison group, model group and sham operation group in middle cerebral artery occlusion (MCAO)model of rats. Results At 6h, the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group , comparison group and sham operation group was increased than that in moddle group,of which Xingnao Kaiqiao group increased obviously(P < 0.05). At 24h, as compared with modlegroup, the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group and comparison group increased significantly (P < 0.05). Conclusion Xingnao Kaiqiao Acupuncture therapy could raise the expression of Peroxiredoxin Ⅵ in focal cerebral ischemia of rats, and the effect is better that of traditional acupuncture therapy in the earlier period.It is benefit to elevating the ability of antioxygen of focal cerebral ischemia of rats.

On the quantitative analysis of focal ischemic cerebral infarction by TTC staining / 生物医学工程学杂志

It is known that ischemic cerebrovascular disease is causing enormous harm to human health on account of the resultant high morbidity and disability rate. In this connexion, the anticipated target is to control the size of focal ischemic cerebral infarction, which is also an important method for judgment of therapeutic efficacy. The key question is to survey the size accurately and objectively; at the same time, the quantitative analysis of focal ischemic cerebral infarction is the pivotal question affecting the experiment conclusion and the reliability level. In this paper are introduced and summarized the methods being recently and commonly used in survey and computation, and the studies made on quantitative analysis of focal ischemic cerebral infarction by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining method. Also are summarized the principles of dyeing in TTC method, the preparatory work, and the commonly used method of surveying and computation. It is the intent of this review to provide relevant data and suggestion for research workers.

Randomized Controlled Trial on Mind-refreshing and Orifice-opening Needling Method and Cerebral Infarction / 针灸推拿医学（英文版）

Objective: To observe the clinical effect of treating remission-stage cerebral infarction with mind-refreshing and orifice-opening needling method. Method: Six hundred cases of cerebral infraction were randomized on the basis of disease phase. The 234 cases in remission stage were randomized into treatment group (116 cases) and control group (118 cases). Besides routine Western therapies, the cases in the treatment group were combined mind-refreshing and orifice-opening needling method and the cases in the control group were combined with conventional needling method. The treatment was done once every day for 4 weeks. The follow-up was done for six months. Result: the baseline material in the two groups has good compatibility (P>0.05) and the treatment group is better than the control group (P<0.05). Conclusion: the mind-refreshing and orifice-opening method is safe and act to improve symptoms of patients during remission stage, reduce disability, prevent disease progression and improve quality of life.

Essence of Needling Technique on Certain Points by Prof. SHI Xue-min / 针灸推拿医学（英文版）

Prof. SHI Xue-min is the first one who proposes the theory of quantitative needling manipulation. This theory requires corresponding quantitative marker of each point. In order to meet this quantitative requirement, he set up strict and rigorous needling methods for each point. Part of his experience in this regard is introduced in this article.

Acupuncture Effects on Endocranial Hemodynamics of Patients with Takayasus Arteritis(Brachiocephalic Artery Type) / 针灸推拿医学（英文版）

Objective: To discuss the action mechanisms of Takayasus arteritis (brachiocephalic artery type) of acupuncture. Methods: We applied acupuncture therapy with the principle of warming Yang and supplementing Qi, removing obstruction in the meridians and recovering pulse, to treat patients with takayasus arteritis (brachiocephalic artery type). Before and after treatment, we detected the TCD (Transcranial Doppler) changes of the average velocity of blood flow (AVBF) and arterial pulsatility index (PI) of related arteris. Results: After treatment,acupuncture and moxibustion can regulate the abnormal blood flow rate in endocranial correlated arteries by two sides, in the AVBF while, elevate the elasticity of endocranial vessels.Conclusion: Acupuncture and moxibustion can regulate cerebrovascular function of patients with takayasus arteritis (brachiocephalic artery type), increase the perfusion of cerebral blood flow, and adjust abnormal state of endocranial hemodynamics. This is possibly the important action mechanisms acupuncture in treating this disorder.

The intervention effect of traditional Chinese medicine on meningeal nuclear factor expression of rats suffering from migraine / 生物医学工程学杂志

This animal experiment was made to probe into the mechanism by which traditional Chinese medicine interferes with the meningeal nuclear factor of rats suffering from migraine. The adult SD rats were selected, and the Cristina Tassorelli method was adopted for the preparation of the migraine models, which were classified into the normal group, model group and traditional Chinese medicine group respectively, and the latter two groups were further divided, in terms of intervals, into 0.5 h, 1.5 h, 2.5 h and 4 h groups, with the model group as control. Real-time quantitative RT-PCR technology was employed to test and measure the gene expression of meningeal nuclear factor of rats. The results showed that when the level of NF-kappaB mRNA expression in the model group was compared with that in 0.5 h group, 1.5 h group and 2.5 h group, there was no significant difference (P > 0.05), but when compared with that in the 4h group, there was significant difference (P < 0.05). Intervention by traditional Chinese medicine in the gene expression of meningeal nuclear factor of model rats suffering from migraine can restrain at different degree the expression of abnormally increasing miningeal nuclear factor NF-kappaB mRNA, which suggests that the intervention by traditional Chinese medicine may enable improvement and treatment of the obstacle of the constriction and dilation system of cerebral blood vessels through restraining excessive expression of meningeal NF-kappaB genes. This conclusion could serve as a theoretical basis for developing traditional Chinese medicine, which takes the NF-kappaB as the target point and the treatment time window, and have underpins an objective approach to the clinical treatment of migraine.

Effect of xingnao kaiqiao acupuncture method on stroke of different syndrome types / 中国组织工程研究

BACKGROUND: Xingnao kaiqiao acupuncture is a series of systematic acupuncture methods with yin channels in domination, assisted by specified standards of quantity manipulation, which was founded according to the pathogenesis of stroke, and it is effective in treating stroke.OBJECTIVE: To observe the effects of xingnao kaiqiao acupuncture on blood lipids and blood viscosity in stroke patients of different syndrome types.DESIGN: A comparative observation.SETTINGS: Institute of Acupuncture and Moxibustion, and Special Care Ward of Acupuncture, the First Affiliated Hospital of Tianjin College of Traditional Chinese Medicine.PARTICIPANTS: Totally 750 stroke inpatients (466 males and 284 females) were selected from the Special Care Ward of Acupuncture, the First Affiliated Hospital of Tianjin College of Traditional Chinese Medicine between January and December in 2001, the average age was (64±12) years,the disease course ranged from 2 hours to 3 years. All the patients were informed and agree with the therapeutic program.METHODS: ① The traditional Chinese medicine (TCM) syndrome differentiations of stroke were in reference to the fifth edition of Traditional Chinese Medicine and Internal Medicine. There were 688 cases of stroke of meridian and collateral type and 47 cases of stroke attacking zang-fu organs type. ② Huatuo filiform needles produced by Suzhou [1.0-1.5 cun in length, 0.32-0.38 mm in diameter (No. 30-28)] were used in the xingnao kaiqiao acupuncture. Firstly, the acupoints of bilateral Neiguan were acupunctured perpendicularly for about 0.5-1.0 cun, the reducing manipulation by twirling as well as lifting and thrusting the needle was performed for 1 minute; Secondly, the needle was inserted at Renzhong towards nasal septum for about 0.3-0.5 cun, the manipulation of heavy bird-peck needling was used till the eyeballs were wet or tears fell down; Then Sanyinjiao was acupunctured, the needle was inserted along the medial border of tibia at an angle of 45° with skin for about 1.0-1.5 cun, the reinforcing manipulation by lifting and thrusting the needle was performed till the ipsilateral lower limb had three tics; The acupoint of Weizhong was selected by lying and lifting the leg, the needle was inserted perpendicularly for 0.5-1.0 cun, and the reducing manipulation by lifting and thrusting was applied till the ipsilateral lower limb had three tics; The acupoint of Jiquan was acupunctured at 1 cun below the source point along the channel in avoidance of axillary hairs, the needle was inserted perpendicularly for 1.0-1.5 cun, and the reducing manipulation by lifting and thrusting was applied till the ipsilateral upper limb had three tics; The elbow was flexed to 120° for acupuncture at Chize, the needle was inserted perpendicularly for 1.0 cun, the reducing manipulation by lifting and thrusting was applied till the ipsilateral upper limb had three tics. Twice a day for 7 days as a course. ③ After four courses averagely, the whole blood viscosities were detected with the hemorrheologic detector, and the normal ranges for lowshear, middle-shear and high-shear of whole blood viscosities were 6.50-9.25, 4.35-5.45 and 3.65-4.40 mpa ·s respectively. The levels of total cholesterol and triglyceride were detected with blood lipid detector, and the normal ranges were 3.38-6.5 mmol/L and 0.56-0.147 mmol/L.MAIN OUTCOME MEASURES: The effects of xingnao kaiqiao acupuncture on the blood lipids and blood viscosity in stroke patients of different syndrome types were observed.RESULTS: Totally 690 and 721 cases finished the detections of whole blood viscosities and blood lipids respectively. There were obviously fewer cases with normal whole blood viscosities in the patients with stroke of meridian and collateral than in those with stroke attacking zang-fu organs (10.3%, 15.4%, P ＜ 0.01), but markedly more cases with higher total cholesterol and triglyceride than in those with stroke attacking zang-fu organs (80.5%, 91.5%; 64.1%, 71.3%; P ＜ 0.01).CONCLUSION: The levels of blood lipids in the patients with stroke of meridian and collateral are more abnormal than in those with stroke attacking zang-fu organs, but their blood viscosities are better than in those with stroke attacking zang-fu organs.

The Effect of "Xingnao Kaiqiao" Acupuncture on the Expression of Peroxiredoxin Ⅵ in the Ischemia Cortex of Rats / 医学研究杂志

Objective To investigate and observe the expression of Peroxiredoxin Ⅵ in ischemia cortex of rats and the intervention effect of Xingnao Kaiqiao Acupuncture(XKA) therapy.Methods Through western blotting,we detected the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group,comparison group,model group and sham operation group in middle cerebral artery occlusion(MCAO)model of rats.Results At 6h,the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group,comparison group and sham operation group was increased than that in moddle group,of which Xingnao Kaiqiao group increased obviously(P

Review on evidence based medicine and progress of TCM / 中华中医药杂志

Evidence based medicine(EBM)makes practitioner of the main current medical science change their attitude to alternative medical science, and provides the technology of studies for Traditional Chinese Medicine (TCM) clinical trials. The method of EBM will provide enormous development space and chance for TCM, and promote the proceeding of the modernization and globalization of TCM. At the same time, the research of TCM should not follow the EBM technology completely, it should keep TCM itself characteristic.To know the EBM defect,it can be better utilized.