ABSTRACT
With the improvement of sanitation, the infection rate of hookworm is greatly reduced and the severe infected case is rarely reported. Combined morphological and molecular biological examinations, a severe hookworm infection patient was diagnosed in Department of Laboratorial Examination, Quanzhou First Affiliated Hospital of Fujian Medical University. The morphological methods such as direct fecal smear microscopy, saturated brine flotation and hookworm larvae culture methods were used to identify the eggs and larvae from stool samples of the patient. There were a large number of hookworm eggs in patient's stool samples, and the average count was 60 840 per gram by modified Kato method, which belonged to severe hookworm infection. Meanwhile, to distinguish the hookworm species, the semi-nested RT-PCR assay was employed to detect hookworm internal transcribed spacer series from eggs in patient's stool samples, and the result showed that the hookworm species was confirmed to be Necator americanus.
Subject(s)
Animals , Humans , Ancylostomatoidea/genetics , Feces , Hookworm Infections/diagnosis , Necator americanus/genetics , Polymerase Chain ReactionABSTRACT
Objective To analyze the associated factors on abnormal liver function(LF)in chronic schistosomiaisis japonica patients.MethodsThe clinical data of 120 inpationts with chronic schistosomiasis were collected and analyzed by logistic regression.The relevant treatment methods were summarized.ResultsThe associated factors on abnormal LF of the patients were hepatitis B,alcohol drinking,hyperlipidemia,nonalcoholic fatty liver disease(NAFLD),and obesity,respectively.The ordinary medication for improving liver function was effective to these causes.Praziquantal can be administrated successfully to these patients after LF normalized.ConclusionChronic schistosomiasis patients with abnormal LF but without severe vital organ diseases could be treated with praziquantal after their LF improving.
