ABSTRACT
Background and introduction Periodontal disease is one of the most prevalent chronic conditions that affects the oral cavity. Identifying and predicting biomarkers is essential for the prevention of high-morbidity oral diseases. The genomic interaction network identifies common hub genes involved in crucial protein formation in periodontal inflammation. Diabetes mellitus is a metabolic disorder that has a double-edged sword relationship with periodontitis. Chloride intracellular channel 1 (CLIC1) was identified as a hub gene linking the pathogenesis of periodontitis and diabetes mellitus using a bioinformatic tool. Therefore, this current study aimed to assess the concentration of the pro-inflammatory biomarker CLIC1 in saliva among individuals with periodontal health and those with periodontal disease linked to diabetes mellitus. Materials and methods Differentially expressed genes (DEGs) in periodontitis were identified using datasets retrieved from the Gene Expression Omnibus (GEO) database. DEGs were combined to build the network, and GeneMANIA was used to find and rank the interconnecting genes. CLIC1 was identified as the hub gene, and clinical validation was done using patient samples. The study involved 30 participants. Based on clinical and radiographic periodontal findings, they were split into three groups: healthy (group 1, n=10), with periodontitis but no diabetes mellitus (group 2, n=10), and with periodontitis and diabetes mellitus (group 3, n=10). The collection of saliva samples, followed by quantifying these samples, was performed using an enzyme-linked immunosorbent assay (ELISA). Results From network graph analysis, it was discovered that CLIC1 functions as a hub gene in the majority of toll-like receptor pathways. The mean concentration of CLIC1 in saliva increased consistently as the disease was observed in periodontitis patients and periodontitis patients with diabetes mellitus. Conclusion CLIC1 concentrations were positively correlated with periodontitis in individuals with diabetes. Therefore, CLIC1 could be a diagnostic biomarker for patients with periodontitis. However, large-scale studies are needed to confirm more positive associations.
ABSTRACT
This paper outlines the effect of farming systems with reference to season on the body condition score (BCS) and adaptive profile (physiological, hemato-biochemical, hormonal, enzymatic and reproductive parameters) of Nellore sheep. In trial 1, sixty ewe-lambs were allotted to extensive, semi-intensive, and intensive rearing systems (n = 20) and evaluated for BCS at puberty, mating, 2 weeks pre-lambing and 2, 4, 8, and 12 weeks post-lambing. In trial 2, eighteen rams were distributed evenly to three farming systems (n = 6) and evaluated for physiological, hemato-biochemical, hormonal, enzymatic, and reproductive parameters concerning three seasons. Although the scores did not differ among the groups, the Kruskal-Wallis ranks of BCS revealed a higher energy status of intensive ewes at different physiological conditions. The sheep reared under extensive and semi-intensive systems displayed higher temperature, pulse rate and respiratory rate with predominant effects in summer season. Similarly, both systems exhibited higher WBC and lower haemoglobin, PCV, and RBC contents without affecting MVC, MCH, MCHC, and differential leucocyte count. The percent haemoglobin and RBC count were higher in winter compared to summer months, whereas WBC count followed an exactly opposite pattern. The sheep reared in intensive system showed higher glucose, total protein, albumin, cholesterol, T3, T4, calcium, and phosphorus; however, the globulin, creatinine, uric acid, aspartate amino transferase (AST), alanine amino transferase (ALT), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase, and catalase levels were elevated in extensive and semi-intensive systems. The dartos muscle extension (DME) and scrotum sweating rate (SSR) were higher for the sheep reared under extensive system, especially during summer season; while the seminal parameters viz., total sperm count, progressive sperm motility, and plasma membrane integrity were lower for extensive and semi-intensive sheep. No interactions were noticed for any of the parameters, except for cortisol, DME, and SSR, which showed significant interactions for rearing system vs. season. Our results showed dynamic adaptive mechanisms of the Nellore sheep in relation to different stressors like grazing for long distances, inadequate nutrition, and heat stress, revealing the heat resilient ability in harsh environmental conditions. Further, the analyzed vector plot showed that the AST, GPx, Cortisol, SOD, Catalase, WBC, PR, T4, total abnormalities, and major abnormalities were the major contributors for adapting during combined stressors.
ABSTRACT
Feeding management in dairy animals is crucial for ensuring optimal production apart from making the farming as a whole, a more sustainable activity. In our study we evaluated the production and environmental effects of two different feeding regimens i.e., one dominated by traditional cottonseed meal (CSM) and other with coated urea (slow release urea - SRU) as a replacement for CSM on dairy buffalo production. The SRU at 2% level was evaluated by conducting two different trials using twelve lactating Murrah buffaloes and four adult Murrah buffalo bulls. Neither diet nor dry period management showed any substantial effect on milk components, intakes, nutrients' digestibility coefficients, and nutritive values. The SRU diet revealed increased (P < 0.01) rumen pH, ammonia nitrogen, volatile fatty acids, and microbial nitrogen yield, which were interacted with time of post-prandial sampling. The dynamics of nitrogen metabolism revealed unaltered N-based parameters, except for degradable-N intake and serum urea-N at 3 hr post-feeding. The CSM replacements did not influence (P > 0.05) the residual feed intake, but led to an enhanced milk retention efficiency of nitrogen, calcium, and phosphorus contents, thus reducing their impact on soil pollution and eutrophication of water bodies. Despite an unaltered (P > 0.05) enteric methane emission, SRU diets achieved in decreasing manure methane and nitrous oxide emission. Furthermore, the virtual water flow and lifecycle assessment revealed a water sparing effect and low carbon foot print per unit milk production. In summary, the CSM replacements with SRU could achieve an economical and eco-friendly production system from animal nutrition perspective.
