Consumo, hábitos alimentarios y habilidades culinarias en alumnos de tercero a quinto año básico y sus padres / Food consumption, dietary behaviour and cooking skills in third to fifth grade school-age children and parents

The objective of this study was to describe food consumption, dietary behaviour and culinary habits in third to fifth grade students and their families through surveys and focus groups to implement nutrition education interventions in healthy eating habits at schools. Surveys in parents and children demonstrated that healthy food consumption was under the Chilean Dietary Guidelines recommendations. Consumption of fruits, vegetables and dairy food in parents was less than in children, but non-healthy food consumption was also less in parents. In dietary behavior 22% of children consumed breakfast at home and also at school, and 33% consumed double lunch. Snack was carried from home in 60% of children, but 72% carried money to buy non-healthy snacks at school. Parents said that they had norms related to dietary behavior at home, but 60% watched TV during dinner and 96% had afternoon tea and just 42.2% had dinner. Regarding culinary habits, students had the skills to prepare simple food but did not have the skills to prepare a dish. The mothers said they had the culinary skills but they cook simple food like pasta, rice and potatoes. Focus groups confirmed survey findings, identifying three profiles in mothers and separating eating behaviour during the weekdays and weekend. Its notorious that mothers consuming a healthy diet were those who had more health problems. Barriers to healthy eating were lack of time and money. Surveys and focus groups showed a good access by parents and children to Information and Communication Technologies. In conclusion, it is urgent to design nutrition education programs to create healthy eating habits considering the current situation in food consumption, dietary behaviour and culinary habits in students and their parents.

El objetivo de este estudio fue describir el consumo, los hábitos alimentarios y las habilidades culinarias en alumnos de tercero a quinto año básico y de su familia por medio de encuestas y grupos focales para realizar intervenciones educativas en alimentación saludable en el ámbito escolar. Para ello se realizaron encuestas a alumnos y padres, que se complementaron con grupos focales. Según las encuestas, el consumo de alimentos saludables en alumnos y padres estuvo por debajo de lo recomendado en las Guías Alimentarias. El consumo de frutas, verduras y lácteos en los padres fue menor que en los hijos, pero consumieron menos alimentos no saludables. En hábitos, 22% de los niños tomaba doble desayuno y 33% doble almuerzo. Un 60% llevaba colación de la casa, pero 72% llevaba dinero destinado a compra de alimentos poco saludables. Los padres decían tener normas, pero un 60% veía televisión cuando comía, 96% tomaba once y 42,2% cenaba. En habilidades culinarias, los alumnos podían preparar alimentos simples, pero no un plato de comida. Las madres decían tener habilidades culinarias, pero cocinaban en forma ocasional y comida básica como papas, arroz y fideos. Los grupos focales confirmaron lo anterior, identificando tres perfiles de madres y diferenciando lo que comen en la semana con los fines de semana. Destaca el perfil de madres que se alimenta saludable, que eran las que han tenido un problema de salud. Las barreras para alimentarse bien son el alto costo y la falta de tiempo. Las encuestas y grupos focales mostraron un buen acceso de padres e hijos a las Tecnologías de la Información y Comunicación. Lo anterior demuestra el imperativo de contar con programas de educación nutricional prácticos para crear hábitos de alimentación saludable que consideren la situación actual de consumo, hábitos alimentarios y habilidades culinarias de los alumnos y sus padres.

Aspectos legales de la transfusión de hemoderivados en el paciente crítico pediátrico hijo de padres Testigos de Jehová, a propósito de un caso mediáticamente relevante / Legal aspects of blood-derived transfusion in a pediatric critical patient whose parents are Jehova Witness: with regard to a medically relevant case

Caso: en el mes de agosto del año 2013, se dio lugar un caso en la ciudad de Guayaquil, que llamó la atención a los medios de comunicación. Una jueza, dictaminó una orden judicial (medida cautelar) para la administración de sangre a un neonato ingresado en Unidad de Cuidados Intensivos Neonatales, que requería transfusión sanguínea urgente y que era hijo de padres que negaban la transfusión al menor de edad aduciendo motivos religiosos. Este artículo tiene como objetivos verificar fuentes sobre el caso y realizar una revisión bibliográfica sobre los fundamentos religiosos, bioéticos y legales que sustenten el evento. Para el desarrollo del mismo se realizó entrevistas tanto a la Jueza involucrada, como al procurador jurídico de la Congregación de los Testigos de Jehová del Ecuador, al Asesor Jurídico de la Jefatura de la DINAPEN (Dirección Nacional de la Policía especializada en Niños, Niñas y Adolescentes), y al Director Nacional Tutelar de la Defensoría del Pueblo del Ecuador. Este reporte de caso, además cuenta con una revisión actualizada del tema con el fin de esclarecer las condiciones en las que se debe recurrir a instancias judiciales ante la negativa de los padres de transfusión a menores de edad en condiciones críticas.

Case: in august 2013, a particular medical case called the attention of the Media in the city of Guayaquil. A Judge granted a judicial order (interim measure) for blood transfusion to a newborn child who was an inpatient in the Newborn Intensive Care Unit and needed the treatment urgently. The parents of the Child refused the blood transfusion due to their religious beliefs. The objectives of the present article are to share the interviews of the sources of the case and confirm the event. In addition, a review of the religious, bioethical and legal issues behind the event has been portrayed. Interviews to the Judge involved, the Jehovah's Witness of Ecuador Attorney, the Legal Adviser of DINAPEN (Police Specialized in Children National Management Office), and the National Tutelary Attorney of the Public Defense Office of Ecuador were performed. Furthermore, the article will set the procedure in which legal action shall be sought for the administration of blood transfusions in critical situations when parents deny a blood transfusion in children.

Intervención en alimentación y nutrición para mineros con factores de riesgo cardiovascular, basada en la investigación formativa / Food and nutrition intervention for miners with cardiovascular risk factors, based on formative research

We designed and implemented an intervention to improve the diet of 94 miners with cardiovascular risk of a mining company. The diagnosis included a qualitative study of the desires, motivations and barriers that workers have to lead a healthy life, a survey of food consumption and checking the institutional food service. Anthropometric and biochemical data were provided by the company. Based on the results, the intervention included improving the supply of healthy foods and conducting the communication campaign "Minero 100% Filete", workshops and nutritional counseling for them and their families. After 8 months of intervention we found a significant increase in the consumption of fruits and vegetables and a decreased consumption of fried, cured and red meats. There was no significant change in BMI. However, there were significant decreases in systolic blood pressure, blood glucose levels, triglycerides and an increase in HDL cholesterol. Although the program was well received by the workers, it is acknowledged that there are significant adverse factors which merit improvement.

Se diseñó e implementó una intervención de 8 meses para mejorar la alimentación de 94 mineros con factores de riesgo cardiovascular. El diagnóstico incluyó un estudio cualitativo de los deseos, motivaciones y barreras de los trabajadores, una encuesta de consumo de alimentos y la revisión del aporte de la alimentación institucional. Los datos antropométricos y bioquímicos fueron proporcionados por la compañía minera. Con esta base, se solicitó mejorar la oferta de alimentos saludables y se realizó la campaña comunicacional "Minero 100% Filete", consejería nutricional, y talleres con las familias. Al finalizar la intervención se observó un aumento significativo del consumo de frutas y verduras y una disminución del consumo de frituras, cecinas y carnes rojas. No hubo cambios significativos en el IMC. Se registraron disminuciones significativas en la presión arterial sistólica, glicemia, triglicéridos y un aumento del colesterol HDL. El programa fue bien aceptado por los trabajadores, pero se reconocen factores ambientales adversos importantes de mejorar.

Adenoviral-mediated gene transfer into bone marrow: an effective surgical technique in rat.

BACKGROUND: The role of transforming growth factor-beta 1 (TGF-ß1) in the onset of bone marrow fibrosis has been confirmed in some animal models. To further understand the genetic expression of some myeloproliferative disorders affecting marrow stem cells, however, it is necessary to develop a specific and reliable procedure to deliver modified adenoviral vectors into the bone marrow cavity. The aim of this paper is to report a surgical technique designed to deliver an adenoviral vector-mediated gene expressing TGF-ß1 into the bone marrow of rat femurs. METHODS: Forty-two Sprague-Dawley rats were used in the study. Rat femurs were exposed and the compact and trabecular bones at the proximal head removed. An intrabone marrow injection of a mutated TGF-ß1 adenoviral vector, a null adenoviral vector, or PBS was delivered into the bone. Three groups were accounted (n = 14 per group): fibrogenic and positive and negative controls. The quality of the surgical entrance was assessed by means of computerized tomography and histological changes were assessed by histochemistry. The concentration of TGF-ß1 in the bone marrow was determined by ELISA. RESULTS: The surgical technique was conducted under ideal timing (approx. 10 min) and no surgical or postsurgical complications were observed. Computerized tomography revealed no changes in the bone tissue and a clean entrance was delimited through the bone to the bone marrow. HE and Masson's trichrome staining indicated highly fibrotic areas in the profibrotic group and bone marrow lavage reported a significantly higher concentration of TGF-ß1 (p < 0.05) in that same group. CONCLUSIONS: The present study confirmed that the proposed surgical technique is an effective method to deliver adenoviral vectors into the femoral bone marrow to investigate the physiopathology of bone marrow fibrosis in rats.

Prostaglandin E2 affects osteoblast biology in a dose-dependent manner: an in vitro study.

OBJECTIVE: This study aimed to determine in vitro how exogenous PGE(2) affects the expression of genes in cultured osteoblasts by relative quantitation PCR. DESIGN: Cultured osteoblasts were exposed to 10(-3)M, 10(-5)M or 10(-7)M PGE(2) over 5, 10, 15 and 20 days. RESULTS: RANKL expression was higher after 5 days of exposure (p<0.05), but thereafter reduced in those treated with the two lower doses of PGE(2) (p<0.01). RANKL/OPG ratio reported in favour of OPG gene expression and alkaline phosphatase gene expression increased in osteoblasts exposed to the two lower doses of the eicosanoid after 15 days. Conversely, prostaglandin E synthase, a cytokine produced during PGE(2) synthesis, gene expression was significantly reduced at 15 and 20 days (p<0.01 and 0.05 respectively). The results from this study add to the current knowledge of the mechanisms by which PGE(2) modulates the osteoblast biology in a dose-dependent manner. CONCLUSIONS: It is proposed that PGE(2)at a low dose switch osteoblast's biology in favour of bone apposition by: first, inducing a significantly higher OPG gene expression overwhelming RANKL gene expression; second, reducing PGEs synthesis; and third, increasing ALP gene expression. An opposite effect is expected when the concentration of the eicosanoid overpass certain levels.

La técnica de incidentes críticos: una herramienta clásica y vigente en enfermería / The critical incidents technique: a classic and effective tool in nursing

Este artículo tiene como objetivo presentar una visión actualizada de la técnica de incidentes críticos, destacando que es una valiosa técnica de investigación cualitativa, flexible, relativamente fácil de aplicar, que permite comprender la interacción de las acciones de los trabajadores en el complejo contexto de la salud y es ampliamente utilizada en salud y en enfermería. En esta revisión se describe su origen, sus principales características, luego se explica cada una de las etapas básicas de su aplicación y también se analiza sus ventajas y desventajas. Finalmente, se discute su contribución a la investigación y su empleo en enfermería para evaluar acciones de intervención en centros de salud.

This paper present an up-to-date vision of the critical incidents technique, highlighting that it is a valuable technique for qualitative investigation, flexible, of relative easy application and broadly used in nursing contexts. This technique also allows understanding the interaction between the actions of people in complex area of health context. In this review, its origin and main characteristics are described. Then a step-by-step explanation is given regarding the use of the technique, and also its advantages and disadvantages are analyzed. Finally, a discussion is presented of how it can contribute to research activities and to the assessment of intervention actions in health organizations.

ER: YAG laser for composite removal after bracket debonding: a qualitative SEM analysis.

OBJECTIVE: To qualitatively compare the effectiveness of the Er:YAG laser with conventional methods for removing the composite remnants and the enamel ablation produced after bracket debonding. MATERIALS AND METHODS: Brackets were bonded on 12 extracted premolars and the composite remnants were removed by 3 different methods: tungsten-carbide bur and 2 Er:YAG laser intervals. Four other premolars were used as a control group. Samples were analyzed with scanning electron microscopy (SEM) and the amount of composite remaining on the teeth and the amount of enamel ablated on each sample were qualitatively ranked by 3 examiners. The results were statistically analyzed by the Tukey test. RESULTS: The Er:YAG laser performed significantly better than the conventional technique for removing the composite remnants, but the amount of enamel ablation produced was significantly higher. CONCLUSIONS: The Er:YAG laser can be used to remove composite remnants after orthodontic bracket debonding, but further studies are required to determine the ideal specifications of this type of laser to reduce the amount of enamel ablation produced under the specifications used in this study.

Buscando una curva general de respuesta a la quimioterapia basada en la evaluación radiológica con criterio RECIST / Looking for a general response curve to chemotherapy based on radiological evaluation with RECIST criteria

Radiological evaluation of response to chemotherapy can be carried out by applying the RECIST (Response Evaluation Criteria in Solid Tumors) criteria, currently considered the most objective method for monitoring lesions. Aimed at finding a general response curve, 21 patients with lung and kidney tumors, as well as colorectal carcinoma were studied between 2006 and 2008. Statistical proceedings included variance (ANOVA), co-variance (ANCOVA) and logarithmic regression analysis. Target-lesion size reduction, duration of response and overall response of different types of tumors did not differ significantly A general response curve was obtained: log (RR)= 1,897-0,0281, where RRt is the relative RECIST count, and t is time (expressed in months). As an expected outcome -because of variety of drugs, type of tumors and variability rates in individual response- the determination coefficient in regression was 10.9 percent. However, it constitutes a first approach to solid tumors response and proves the accuracy and usefulness of RECIST methodology.

La evaluación radiológica de respuesta a la quimioterapia se puede hacer con el criterio RECIST, considerado actualmente el método más objetivo de seguimiento de lesiones. Con el objetivo de buscar una curva general de respuesta, entre los años 2006 y 2008 estudiamos 21 pacientes con tumores pulmonares, renales y cáncer colo-rectal. La estadística consistió en análisis de varianza (ANOVA), covarianza (ANCOVA) y regresión logarítmica. La reducción en tamaño y el tiempo de respuesta no difirieron significativamente, así como tampoco la respuesta global de los diferentes cánceres. Se obtuvo una curva general de respuesta: log (RR)= 1,897 -0,028 t, (RR= evaluación RECIST actual dividida por inicial y t= tiempo en meses). El coeficiente de determinación de la regresión fue 10,9 por ciento, esperable por la diversidad de fármacos, tipo de tumor y respuesta individual; sin embargo constituye una primera aproximación a la respuesta esperada mostrando la utilidad y objetividad del método.

La confiabilidad en el líder: un estudio sobre las enfermeras jefes de un hospital / The trustworthiness in the leader: a study on head nurses of a hospital

La confiabilidad en el líder es un concepto multidimensional que hace complejo establecer cuáles son sus antecedentes. Por ello, se buscó obtener consenso entre enfermeras y técnicos paramédicos sobre cuáles son las categorías de conductas que manifiestan los líderes y que favorecen la percepción de que son confiables. Además, se relacionó las categorías de conducta obtenidas con los principales antecedentes de la confiabilidad en los líderes. Se utilizó la técnica delphi y participaron en total 67 funcionarios de diversos servicios de un hospital y fueron distribuidos en 4 grupos. Se obtuvo que la categoría de conducta más importante son la objetividad e imparcialidad en la toma de decisiones de los líderes, lo cual reafirma la importancia de la integridad como antecedente de la confiabilidad de éstos. Finalmente, se discute sobre cómo fortalecer la confiabilidad de los líderes.

The trustworthiness in the leader is a multidimensional concept that makes it complex to determine which are its background. As a result, a consensus among nurses and technical paramedics was considered on the categories of behaviour which the leader manifests and which facilitate the perception that they are trustworthiness. Subsequently, behaviour categories were related with the main records of the trustworthiness in the leaders. The Delphi technique was used and a total of 67 workers of diverse services of a hospital were the participants and they were distributed in 4 groups. It was found that the most important categories of behaviour is the leaders' impartiality and objectivity in decision making process, which reaffirms the importance of integrity and history of reliability of these. Finally, the ways to strengthen the trustworthiness in the leaders are discussed.

Prevalence of malocclusions in a young Brazilian population.

Malocclusions are generally treated in adolescents and adults, but they are established at an early age. The purpose of this study was to determine the prevalence of malocclusions in a young Brazilian population. The sample included 926 children, 8 to 12 years old, attending 5 public schools in the state of Goias, Brazil The type of occlusion was visually determined during the oral exam and statistical analysis, Chi-square test, was performed to correlate the prevalence of malocclusion with gender and with age. 819 patients out of the 926 patients had some type of malocclusion. From those, 513 patients had a class I malocclusion, 201 patients were classified as class II malocclusion, and 105 patients were class III malocclusion. Vertically, 62 patients showed a deep bite and 61 patients had an open bite. Transversely, 40 patients presented a bilateral posterior crossbite, 54 patients had a posterior crossbite on the left side, and 39 patients had a posterior crossbite on the right side. No significant correlation between gender and malocclusions was found and the number of patients with malocclusions between boys and girls were similar. Considering the three spatial planes, there is a high prevalence of malocclusions among the young Brazilian population. Therefore, the dental community must improve health policies and treat malocclusions earlier.

Dimensional changes in dental arches after treatment with a prefabricated functional appliance.

The purpose of this study was to determine the effect of the T4K, a prefabricated functional appliance, on the transverse and anterior-height dimensions of the maxillary and mandibular dental arches. Dimensions before and after treatment were measured on the sample, then, natural growth was subtracted from the treatment effects and compared with twice the error of the method. A clinically significant increase of both dimensions was observed in the maxilla and mandible when Class II malocclusion patients were treated with the T4K. Therefore, this retrospective study demonstrates that T4K is a valid treatment choice at an early age when transverse expansion is part of the treatment goal.

Prostaglandin E2 enhances transforming growth factor-beta 1 and TGF-beta receptors synthesis: an in vivo and in vitro study.

The aims of this study were to determine how Prostaglandin E2 (PGE2) locally applied affected the immunodistribution of latent transforming growth factor-beta 1 (TGF-beta1), and how the eicosanoid modified TGF-beta1 release and TGF-beta receptors gene expression in cultured osteoblasts. PGE2 locally delivered on the rat mandible at doses of 0.1 and 0.05 mg/day, but not 0.025 mg/day, over 20 days significantly increased latent TGF-beta1 immunodistribution (P<0.001), comparing with a placebo-treated group. Cultured osteoblasts stimulated with 10(-5) or 10(-7)M PGE2 significantly varied the level of activated TGF-beta1 released into supernatants at different experimental periods compared with negative and positive controls. TGF-beta receptor type I gene expression was significantly increased in osteoblasts (P<0.01) after 10 days of treatment with 10(-5) and 10(-7)M PGE2, whereas 10(-3) M PGE2 produced the opposite effect. It is concluded that PGE2 may stimulate bone deposition by affecting TGF-beta pathway. This effect on the pathway appears to be dose-dependent.

Severe anaphylactic reaction during the second infusion of infliximab in a patient with psoriatic arthritis.

A 33-yer-old woman with no history of atopy, diagnosed of psoriatic arthritis, received 200 mg I.V. infliximab, with previous oral administration of loratadine and betamethasone, that was well tolerated. Two minutes after a second infusion two weeks later, with the same pretreatment, the patients suffer dyspnea, laryngeal spasm, generalized tremor, vomiting, hypotension, sinusal tachycardia, anxiety and hyposemia. She recovered in 45 minutes, after the administration of I.V. hydrocortisone, chloropyramine, adrenaline and oxygen. Several reports of infliximab-induced anaphylactic reactions have been published, especially in patients with Crohn's disease, that have been attributed to a type I (acute or delayed) hypersensitivity reaction mechanism.

Severe anaphylactic reaction during the second infusion of infliximab in a patient with psoriatic arthritis

A 33-yer-old woman with no history of atopy, diagnosed of psoriatic arthritis, received 200 mg I.V. infliximab, with previous oral administration of loratadine and betamethasone, that was well tolerated. Two minutes after a second infusion two weeks later, with the same pretreatment, the patients suffer dyspnea, laryngeal spasm, generalized tremor, vomiting, hypotension, sinusal tachycardia, anxiety and hyposemia. She recovered in 45 minutes, after the administration of I.V. hydrocortisone, chloropyramine, adrenaline and oxigene. Several reports of infliximab-induced anaphylactic reactions have been published, especially in patients with Crohn's disease, that have been attributed to a type I (acute or delayed) hypersensitivity reaction mechanism

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Local application of prostaglandin E2 reduces trap, calcitonin receptor and metalloproteinase-2 immunoreactivity in the rat periodontium.

It has been shown that prostaglandin E2 (PGE2) locally released adjacent to the mandible over a 20-day period increases alveolar bone area, in part, due to a reduction in the percentage of eroded surface. To determine the effect of PGE2 on alveolar bone resorption, left mandibles from 24 Lewis rats were treated over a 20-day period with a local application of PGE2 (0.1, 0.05 or 0.025 mg/day) or placebo. The right side served as the non-treated matched control. Tissue sections were stained for tartrate resistant acid phosphatase (TRAP) calcitonin receptor (CTR) and metalloproteinase-2 (MMP-2). Matched samples were analysed by Wilcoxon matched pairs test and, a non-parametric one-way analysis of variance compared groups of treatment. Those tissues treated with PGE2 at doses of 0.1 and 0.05 mg/day showed significantly reduced numbers of TRAP and CTR-positive multinucleated cells compared with matched controls (p<0.005), as well as significantly reduced numbers of TRAP- and CTR-positive multinucleated cells when compared with the placebo-treated group (p<0.001). The number of periodontal ligament cells expressing MMP-2 was also significantly reduced in tissues treated with the two higher doses of PGE2 (p<0.001) comparing with both matched controls and the placebo-treated group. Following a 20-day period, locally released PGE2 at doses of 0.1 and 0.05 mg/day appears to affect alveolar bone resorption in the periodontium of rats, as the number of multinucleated cells expressing TRAP and CTR are significantly reduced. Furthermore, the same doses of PGE2 also significantly reduced the expression of MMP-2 by the periodontal cells.

Prostaglandin E2 enhances alveolar bone formation in the rat mandible.

Prostaglandin E2 (PGE2) induces bone formation in stress-bearing bones. The mandible, a stress-bearing bone, is loaded daily during mastication. The aim of this study was to determine if PGE2 delivered locally to the mandible over 20 days enhances alveolar bone deposition. In 18 Lewis rats, controlled-release pellets containing PGE2 were implanted on the buccal aspect on the left-hand side of the mandible, mesial to the root of the first molar. Controlled-release pellets locally delivered 0.1, 0.05, or 0.025 mg/day of PGE2. The right side of the mandible was used as a matched control for each animal. Six sham-treated animals were implanted with a placebo pellet. On days 7 and 19, animals were injected with the bone markers tetracycline and calcein, respectively. On day 21, animals were sacrificed and undecalcified tissues obtained for morphometrical analysis. Morphometrical measurements were analyzed by paired t test to determine differences between the matched samples and one-way ANOVA to compare the different treatment groups. A significant increase in alveolar bone area was observed in mandibles treated with 0.1 and 0.05 mg/day when compared with matched controls and the placebo group. This was accompanied by a significant increase in alveolar bone height and width. The proportions of double-labeled surface (dLS), the mineral apposition rate (MAR), and bone formation rate (BFR) were significantly increased in mandibles treated with the two higher doses of PGE2. The proportion of resorptive surface (RS) was significantly reduced in these two groups. It is concluded that PGE2 induces alveolar bone formation in the mandible when locally delivered at a dose of 0.1 or 0.05 mg/day for 20 days.

Periodontal treatment needs in a native island community in Colombia determined with CPITN.

AIMS: To identify the prevalence and different degrees of periodontal disease in an isolated community (Isla Grande, Colombia) with no dental services and low educational level with the use of CPITN, and to establish periodontal treatment needs in different age groups. RESULTS: Of 116 people examined, 0.9% were in periodontal health (CPITN value 0), 18.1% had gingival bleeding (CPITN value 1), 51.7% had supra or subgingival calculus (CPITN value 2), 18.1% presented pockets 3.5-5.0 mm deep (CPITN value 3), and 11.2% had pathological pockets of 5.5mm or deeper (CPITN value 4). No clear differences were observed between sexes. CONCLUSIONS: This study shows that 81% of the sample has some type of periodontal treatment need, with 69.8% of them requiring periodontal treatment that may be supplied by a hygienist and 11.2% requiring specialised treatment. Implementation of oral health education and oral prevention programmes was recommended to the authorities for this community.

Incisor disocclusion in rats affects mandibular condylar cartilage at the cellular level.

UNLABELLED: The effect of altered occlusion on the mandibular condylar cartilage remains unclear. OBJECTIVE: This study investigated the effect of unilateral incisor disocclusion on cartilage thickness, on mitotic activity and on chondrocytes maturation and differentiation in the mandibular condylar cartilage of rats. DESIGN: The upper and lower left incisors were trimmed 2mm every second day in five rats. In other five rats, the incisor occlusion was not altered. Condylar tissues from both sides of each mandible were processed and stained for Herovici's stain and immunohistochemistry for bromodeoxyuridine (BrdU), transforming growth factor-beta1 (TGF-beta1), alkaline phosphatase (ALP) and osteocalcin (OCN). Measurements of cartilage thickness and the numbers of immunopositive cells for each antibody were analysed by one-way analysis of variance (ANOVA). RESULTS: No significant differences were observed in cartilage thickness after 7 days of unilateral incisor disocclusion. However, the numbers of immunopositive cells for BrdU as a marker of DNA synthesising cells, TGF-beta1 as a marker of chondrocytes differentiation, and ALP and OCN as markers of chondrocytes maturation, were significant higher in the cartilage cells on both sides when incisor occlusion was unilaterally altered. Interestingly, alkaline phosphatase was highly expressed on the condylar side of incisor disocclusion, whereas osteocalcin was highly expressed on the side opposite to the incisor disocclusion. CONCLUSIONS: It is demonstrated that after 7 days, unilateral incisor disocclusion affects the mandibular condylar cartilage at the cellular level by increasing the mitotic activity and by accelerating chondrocytes maturation. Chondrocytes maturation appears more accelerated on the side opposite to incisor disocclusion.

Estudio comparativo de calidad de leche fluida y en polvo / Comparative study of the quality of processed stored milks

Introducción: La leche de vaca forma parte de la alimentación del niño mayor de 1 año. La industria láctea utiliza procesos térmicos que pueden afectar algunos nutrientes de la leche. Objetivo: evaluar el efecto del proceso de elaboración de leches pasteurizada, fluida UHT y en polvo, sobre la calidad químico nutricional y organoléptica. Método: Muestras de los 3 tipos de leche, obtenidas de una materia prima común, a los 0 y 90 días fueron sometidas a análisis de humedad, proteínas (N x 6,38), grasas, cenizas, calcio, fósforo, acidez, vitamina A, lisina disponoble, perfil de ácidos grasos, índice de peróxidos e índice tiobarbitúrico y de energía. Se evaluó también apariencia, color, aroma, sabor, acidez, rancidez, viscosidad y aceptabilidad. Análisis estadístico: se utilizó cálculo de promedio aritmético y desviación estánda, diferencia entre promedios, test de análisis de varianza y test t de Student a los promedios de análisis químicos y test de Duncan a las pruebas sensoriales. Resultados: La evaluación realizada a tiempo cero, para todas las leches, y después de 90 días de almacenamiento a temperatura ambiente, para leche UHT y en polvo, no mostró cambios en los macronutrientes para ningún tipo de leche siendo similares al expresarlo en base líquida con cantidades comparables de sólidos. En cuanto a micronutrientes, hubo una disminución significativa en vitamina A y lisina disponible en leche en polvo y en ácido linoleico para leche UHT a los 90 días. Sensorialmente, todas las leches fueron evaluadas como de buena calidad, buena aceptabilidad, sin diferencias notables ni entre ellas ni con un control siendo, en algunos atributos de calidad y en aceptabilidad, mejor evaluada la leche UHT. Conclusión: Los cambios químico-nutricionales encontrados en ambas leches (fluidas UHT y en polvo), que son inherentes a los procesos utilizados, no debieran afectar la situación nutricional del niño cuando la leche está integrada a una alimentación balanceada.

Broad band spectral measurements of EEG during emotional tasks.

Broad band spectral measurements were calculated on EEG recordings of 40 healthy male university students during emotional tasks using the emotion induction technique. Emotional stimuli were sentences with positive valence (joy of love), with negative valence (frustration) and neutral situation. Broad band measurements showed that delta and beta bands reflected better the differences by emotional state. Hemispheric differences associated to emotional states were obtained only for mean frequency data. Higher delta frequencies at left frontopolar, right frontal, parietal and temporal and higher beta frequencies at left frontotemporal and temporal were related to love. Meanwhile higher delta frequencies especially at left frontopolar and temporal regions and lower beta frequencies at midline frontal (Fz) and central (Cz) leads were associated to frustration. These findings suggest that narrow band measurements may reflect more efficiently EEG changes during emotional tasks.