Development of Vero cell miniculture assay for detection of heat-labile enterotoxin of Escherichia coli and its significance.

In the present study the Vero cell miniculture assay was developed for the detection of heat-labile enterotoxin of E. coli. The test was found to be reliable and efficient and was comparable with other tissue culture assays. The new cell lines BHK21, MDBK, LM and CK included in the present study did not respond positively to the E. coli toxins.