ABSTRACT
Respiratory metabolism depends on mitochondrial DNA, yet the mechanisms that ensure the inheritance of the mitochondrial genome are largely obscure. Recent studies with Saccharomyces cerevisiae suggest that distinct factors mediate the active segregation of mitochondrial DNA during mitotic growth. The identification of the proteins required for the maintenance of the mitochondrial genome provides clues to the mechanisms of, and molecular machinery involved in, mitochondrial DNA inheritance.
Subject(s)
DNA, Mitochondrial/genetics , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/physiology , DNA, Mitochondrial/physiology , Mitochondria/genetics , Mitochondria/physiology , MitosisABSTRACT
Mitochondrial morphology and function depend on MGM1, a Saccharomyces cerevisiae gene encoding a dynamin-like protein of the mitochondrial outer membrane. Here, we show that mitochondrial fragmentation and mitochondrial genome loss caused by lesions in MGM1 are suppressed by three novel mutations, gag1, gag2, and gag3 (for glycerol-adapted growth). Cells with any of the gag mutations displayed aberrant mitochondrial morphology characterized by elongated, unbranched tubes and highly fenestrated structures. Additionally, each of the gag mutations prevented mitochondrial fragmentation caused by loss of the mitochondrial fusion factor, Fzo1p, or by treatment of cells with sodium azide. The gag1 mutation mapped to DNM1 that encodes a dynamin-related protein required for mitochondrial fission. GAG3 encodes a novel WD40-repeat protein previously found to interact with Dnm1p in a two-hybrid assay. Gag3p was localized to mitochondria where it was found to associate as a peripheral protein on the cytosolic face of the outer membrane. This association requires neither the DNM1 nor GAG2 gene products. However, the localization of Dnm1p to the mitochondrial outer membrane is substantially reduced by the gag2 mutation, but unaffected by loss of Gag3p. These results indicate that Gag3p plays a distinct role on the mitochondrial surface to mediate the fission of mitochondrial tubules.
Subject(s)
Fungal Proteins/genetics , GTP-Binding Proteins , Membrane Proteins/genetics , Mitochondria/physiology , Mitochondrial Proteins , Saccharomyces cerevisiae Proteins , Adaptation, Biological , Cell Compartmentation , Fungal Proteins/metabolism , GTP Phosphohydrolases/genetics , Glycerol/metabolism , Mitochondria/drug effects , Mitochondria/ultrastructure , Protein Binding , Saccharomyces cerevisiae/physiology , Saccharomyces cerevisiae/ultrastructure , Sodium Azide/pharmacology , Suppression, Genetic , Two-Hybrid System TechniquesABSTRACT
Cytochrome c released from vertebrate mitochondria engages apoptosis by triggering caspase activation. We previously reported that, whereas cytochromes c from higher eukaryotes can activate caspases in Xenopus egg and mammalian cytosols, iso-1 and iso-2 cytochromes c from the yeast Saccharomyces cerevisiae cannot. Here we examine whether the inactivity of the yeast isoforms is related to a post-translational modification of lysine 72, N-epsilon-trimethylation. This modification was found to abrogate pro-apoptotic activity of metazoan cytochrome c expressed in yeast. However, iso-1 cytochrome c lacking the trimethylation modification also was devoid of pro-apoptotic activity. Thus, both lysine 72 trimethylation and other features of the iso-1 sequence preclude pro-apoptotic activity. Competition studies suggest that the lack of pro-apoptotic activity was associated with a low affinity for Apaf-1. As cytochromes c that lack apoptotic function still support respiration, different mechanisms appear to be involved in the two activities.
Subject(s)
Apoptosis , Cytochrome c Group/metabolism , Cytochromes c , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/metabolism , Amino Acid Sequence , Animals , Horses , Lysine/analogs & derivatives , Lysine/metabolism , Methylation , Mitochondria/metabolism , Models, Molecular , Molecular Sequence Data , Oocytes , Peptide Hydrolases/metabolism , Protein Isoforms , Sequence Homology, Amino Acid , XenopusSubject(s)
Drosophila Proteins , Fungal Proteins/physiology , GTP Phosphohydrolases/physiology , GTP-Binding Proteins/physiology , Membrane Proteins/physiology , Mitochondria/physiology , Saccharomyces cerevisiae Proteins , Animals , Fungal Proteins/metabolism , GTP Phosphohydrolases/metabolism , GTP-Binding Proteins/metabolism , Insect Proteins/metabolism , Insect Proteins/physiology , Membrane Proteins/metabolism , Mitochondrial Proteins , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae/physiologyABSTRACT
The smm1 mutation suppresses defects in mitochondrial distribution and morphology caused by the mdm1-252 mutation in the yeast Saccharomyces cerevisiae. Cells harboring only the smm1 mutation themselves display temperature-sensitive growth and aberrant mitochondrial inheritance and morphology at the nonpermissive temperature. smm1 maps to RSP5, a gene encoding an essential ubiquitin-protein ligase. The smm1 defects are suppressed by overexpression of wild-type ubiquitin but not by overexpression of mutant ubiquitin in which lysine-63 is replaced by arginine. Furthermore, overexpression of this mutant ubiquitin perturbs mitochondrial distribution and morphology in wild-type cells. Site-directed mutagenesis revealed that the ubiquitin ligase activity of Rsp5p is essential for its function in mitochondrial inheritance. A second mutation, smm2, which also suppressed mdm1-252 defects, but did not cause aberrant mitochondrial distribution and morphology, mapped to BUL1, encoding a protein interacting with Rsp5p. These results indicate that protein ubiquitination mediated by Rsp5p plays an essential role in mitochondrial inheritance, and reveal a novel function for protein ubiquitination.
Subject(s)
Adaptor Proteins, Signal Transducing , Cell Cycle Proteins , Fungal Proteins/metabolism , Mitochondria/physiology , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/cytology , Ubiquitin-Protein Ligase Complexes , Ubiquitins/physiology , Amino Acid Substitution , Carrier Proteins/genetics , Carrier Proteins/metabolism , Endosomal Sorting Complexes Required for Transport , Fungal Proteins/genetics , Gene Expression , Genes, Essential/genetics , Genes, Fungal/genetics , Genes, Fungal/physiology , Genes, Suppressor/genetics , Genetic Complementation Test , Intermediate Filament Proteins , Ligases/genetics , Ligases/metabolism , Mitochondria/genetics , Mutation , Phenotype , Protein Binding , Saccharomyces cerevisiae/enzymology , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/growth & development , Suppression, Genetic , Temperature , Ubiquitin-Protein Ligases , Ubiquitins/genetics , Ubiquitins/metabolismABSTRACT
The distribution of mitochondria to daughter cells during cell division is an essential feature of cell proliferation. Until recently, it was commonly believed that inheritance of mitochondria and other organelles was a passive process, a consequence of their random diffusion throughout the cytoplasm. A growing recognition of the reticular morphology of mitochondria in many living cells, the association of mitochondria with the cytoskeleton, and the coordinated movements of mitochondria during cellular division and differentiation has illuminated the necessity for a cellular machinery that mediates mitochondrial behavior. Characterization of the underlying molecular components of this machinery is providing insight into mechanisms regulating mitochondrial morphology and distribution.
Subject(s)
Cell Division , Mitochondria/genetics , Mitochondria/physiology , Animals , Cytoskeletal Proteins/genetics , Cytoskeletal Proteins/physiology , Cytoskeleton/physiology , Dynamins , GTP Phosphohydrolases/physiology , Genes , Humans , Mitochondria/ultrastructure , Movement , Mutation , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/physiology , Saccharomyces cerevisiae/ultrastructureABSTRACT
The mdm17 mutation causes temperature-dependent defects in mitochondrial inheritance, mitochondrial morphology, and the maintenance of mitochondrial DNA in the yeast Saccharomyces cerevisiae. Defects in mitochondrial transmission to daughter buds and changes in mitochondrial morphology were apparent within 30 min after shifting cells to 37 degrees C, while loss of the mitochondrial genome occurred after 4-24 h at the elevated temperature. The mdm17 lesion mapped to MGM1, a gene encoding a dynamin-like GTPase previously implicated in mitochondrial genome maintenance, and the cloned MGM1 gene complements all of the mdm17 mutant phenotypes. Cells with an mgm1-null mutation displayed aberrant mitochondrial inheritance and morphology. A version of mgm1 mutated in a conserved residue in the putative GTP-binding site was unable to complement any of the mutant defects. It also caused aberrant mitochondrial distribution and morphology when expressed at high levels in cells that also contained a wild-type copy of the gene. Mgm1p was localized to the mitochondrial outer membrane and fractionated as a component of a high molecular weight complex. These results indicate that Mgm1p is a mitochondrial inheritance and morphology component that functions on the mitochondrial surface.
Subject(s)
Fungal Proteins/metabolism , GTP-Binding Proteins , Mitochondria/metabolism , Mitochondrial Proteins , Saccharomyces cerevisiae Proteins , Alleles , Base Sequence , Binding Sites/genetics , Conserved Sequence , DNA Primers/genetics , DNA, Fungal/genetics , DNA, Mitochondrial/genetics , Fungal Proteins/genetics , Genes, Fungal , Genome, Fungal , Guanosine Triphosphate/metabolism , Intracellular Membranes/metabolism , Mutation , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , TemperatureABSTRACT
Phb2p, a homolog of the tumor suppressor protein prohibitin, was identified in a genetic screen for suppressors of the loss of Mdm12p, a mitochondrial outer membrane protein required for normal mitochondrial morphology and inheritance in Saccharomyces cerevisiae. Phb2p and its homolog, prohibitin (Phb1p), were localized to the mitochondrial inner membrane and characterized as integral membrane proteins which depend on each other for their stability. In otherwise wild-type genetic backgrounds, null mutations in PHB1 and PHB2 did not confer any obvious phenotypes. However, loss of function of either PHB1 or PHB2 in cells with mitochondrial DNA deleted led to altered mitochondrial morphology, and phb1 or phb2 mutations were synthetically lethal when combined with a mutation in any of three mitochondrial inheritance components of the mitochondrial outer membrane, Mdm12p, Mdm10p, and Mmm1p. These results provide the first evidence of a role for prohibitin in mitochondrial inheritance and in the regulation of mitochondrial morphology.
Subject(s)
Fungal Proteins/genetics , Membrane Proteins/genetics , Mitochondria/metabolism , Proteins/genetics , Repressor Proteins , Saccharomyces cerevisiae/genetics , Fungal Proteins/metabolism , Intracellular Membranes/metabolism , Membrane Proteins/metabolism , Mutagenesis , Prohibitins , Proteins/metabolism , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae ProteinsABSTRACT
Nuclear and mitochondrial transmission to daughter buds of Saccharomyces cerevisiae depends on Mdm1p, an intermediate filament-like protein localized to numerous punctate structures distributed throughout the yeast cell cytoplasm. These structures disappear and organelle inheritance is disrupted when mdm1 mutant cells are incubated at the restrictive temperature. To characterize further the function of Mdm1p, new mutant mdm1 alleles that confer temperature-sensitive growth and defects in organelle inheritance but produce stable Mdm1p structures were isolated. Microscopic analysis of the new mdm1 mutants revealed three phenotypic classes: Class I mutants showed defects in both mitochondrial and nuclear transmission; Class II alleles displayed defective mitochondrial inheritance but had no effect on nuclear movement; and Class III mutants showed aberrant nuclear inheritance but normal mitochondrial distribution. Class I and II mutants also exhibited altered mitochondrial morphology, possessing primarily small, round mitochondria instead of the extended tubular structures found in wild-type cells. Mutant mdm1 alleles affecting nuclear transmission were of two types: Class Ia and IIIa mutants were deficient for nuclear movement into daughter buds, while Class Ib and IIIb mutants displayed a complete transfer of all nuclear DNA into buds. The mutations defining all three allelic classes mapped to two distinct domains within the Mdm1p protein. Genetic crosses of yeast strains containing different mdm1 alleles revealed complex genetic interactions including intragenic suppression, synthetic phenotypes, and intragenic complementation. These results support a model of Mdm1p function in which a network comprised of multimeric assemblies of the protein mediates two distinct cellular processes.
Subject(s)
Cell Cycle Proteins , Cell Nucleus/genetics , Fungal Proteins/genetics , Mitochondria/genetics , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Alleles , Cell Nucleus/physiology , Chromosome Mapping , Fungal Proteins/physiology , Genes, Fungal , Genetic Complementation Test , Intermediate Filament Proteins , Mitochondria/ultrastructure , Mutation , Open Reading Frames , Phenotype , Sequence Analysis, DNA , Suppression, Genetic , TemperatureABSTRACT
Saccharomyces cerevisiae cells lacking the MDM12 gene product display temperature-sensitive growth and possess abnormally large, round mitochondria that are defective for inheritance by daughter buds. Analysis of the wild-type MDM12 gene revealed its product to be a 31-kD polypeptide that is homologous to a protein of the fission yeast Schizosaccharomyces pombe. When expressed in S. cerevisiae, the S. pombe Mdm12p homolog conferred a dominant-negative phenotype of giant mitochondria and aberrant mitochondrial distribution, suggesting partial functional conservation of Mdm12p activity between budding and fission yeast. The S. cerevisiae Mdm12p was localized by indirect immunofluorescence microscopy and by subcellular fractionation and immunodetection to the mitochondrial outer membrane and displayed biochemical properties of an integral membrane protein. Mdm12p is the third mitochondrial outer membrane protein required for normal mitochondrial morphology and distribution to be identified in S. cerevisiae and the first such mitochondrial component that is conserved between two different species.
Subject(s)
Fungal Proteins/metabolism , Membrane Proteins/metabolism , Mitochondria/metabolism , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/metabolism , Schizosaccharomyces/metabolism , Base Sequence , Cell Membrane/metabolism , DNA, Fungal , Fungal Proteins/genetics , Membrane Proteins/genetics , Mitochondria/ultrastructure , Molecular Sequence Data , Phenotype , Repressor Proteins , Sequence Homology, Amino Acid , Spores, FungalABSTRACT
The Drosophila Fzo protein is required for mitochondrial fusion during a key step in sperm formation. This novel, membrane-bound GTPase defines a family of proteins that may mediate mitochondrial fusion in a variety of eukaryotic cells.
Subject(s)
Drosophila Proteins , Drosophila melanogaster/physiology , GTP Phosphohydrolases/physiology , Insect Proteins/physiology , Membrane Proteins/physiology , Mitochondria/physiology , Spermatogenesis/physiology , Animals , Fertility/physiology , GTP Phosphohydrolases/genetics , Guanosine Triphosphate/metabolism , Humans , Insect Proteins/genetics , Male , Membrane Fusion , Membrane Proteins/genetics , MorphogenesisABSTRACT
Mechanisms mediating the inheritance of mitochondria are poorly understood, but recent studies with the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe have begun to identify components that facilitate this essential process. These components have been identified through the analysis of conditional yeast mutants that display aberrant mitochondrial distribution at restrictive conditions. The analysis of these mutants has uncovered several novel proteins that are localized either to cytoskeletal structures or to the mitochondria themselves. Many mitochondrial inheritance mutants also show altered mitochondrial morphology and defects in maintenance of the mitochondrial genome. Although some inheritance components and mechanisms appear to function specifically in certain types of cells, other conserved proteins are likely to mediate mitochondrial behavior in all eukaryotic cells.
Subject(s)
Mitochondria/genetics , Mitochondria/metabolism , Cell Membrane/chemistry , Cell Membrane/metabolism , Cytoskeleton/chemistry , Cytoskeleton/metabolism , Mitochondria/chemistry , Mitosis/genetics , Models, Biological , Mutation/genetics , Saccharomyces/geneticsABSTRACT
Expression of the proapoptotic protein Bax under the control of a GAL10 promoter in Saccharomyces cerevisiae resulted in galactose-inducible cell death. Immunofluorescence studies suggested that Bax is principally associated with mitochondria in yeast cells. Removal of the carboxyl-terminal transmembrane (TM) domain from Bax [creating Bax (deltaTM)] prevented targeting to mitochondrial and completely abolished cytotoxic function in yeast cells, suggesting that membrane targeting is crucial for Bax-mediated lethality. Fusing a TM domain from Mas70p, a yeast mitochondrial outer membrane protein, to Bax (deltaTM) restored targeting to mitochondria and cytotoxic function in yeast cells. Deletion of four well-conserved amino acids (IGDE) from the BH3 domain of Bax ablated its ability to homodimerize and completely abrogated lethality in yeast cells. In contrast, several Bax mutants which retained ability to homodimerize (deltaBH1, deltaBH2, and delta1-58) also retained at least partial lethal function in yeast cells. In coimmunoprecipitation experiments, expression of the wild-type Bax protein in Rat-1 fibroblasts and 293 epithelial cells induced apoptosis, whereas the Bax (deltaIGDE) mutant failed to induce apoptosis and did not associate with endogenous wild-type Bax protein. In contrast to yeast cells, Bax (deltaTM) protein retained cytotoxic function in Rat-1 and 293 cells, was targeted largely to mitochondria, and dimerized with endogenous Bax in mammalian cells. Thus, the dimerization-mediating BH3 domain and targeting to mitochondrial membranes appear to be essential for the cytotoxic function of Bax in both yeast and mammalian cells.
Subject(s)
Apoptosis , Proto-Oncogene Proteins c-bcl-2 , Proto-Oncogene Proteins/chemistry , Proto-Oncogene Proteins/metabolism , Saccharomyces cerevisiae/physiology , Animals , Cell Line , Cloning, Molecular , DNA Primers , Fluorescent Antibody Technique, Indirect , Humans , Mammals , Mice , Microscopy, Electron , Phenotype , Polymerase Chain Reaction , Proto-Oncogene Proteins/biosynthesis , Rats , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Saccharomyces cerevisiae/growth & development , Saccharomyces cerevisiae/ultrastructure , Transfection , bcl-2-Associated X ProteinABSTRACT
The Schizosaccharomyces pombe mutant, ban5-4, displays aberrant mitochondrial distribution. Incubation of this conditional-lethal mutant at the nonpermissive temperature led to aggregated mitochondria that were distributed asymmetrically within the cell. Development of this mitochondrial asymmetry but not mitochondrial aggregation required progression through the cell division cycle. Genetic analysis revealed that ban5-4 is an allele of atb2 encoding alpha 2-tubulin. Consistent with this finding, cells with the cold-sensitive nda3 mutation in beta-tubulin displayed aggregated and asymmetrically distributed mitochondria after incubation at lowered temperatures. These results indicate that microtubules mediate mitochondrial distribution in fission yeast and provide the first genetic evidence for the role of microtubules in mitochondrial movement.
Subject(s)
Microtubules/physiology , Mitochondria/physiology , Schizosaccharomyces/physiology , Cell Cycle , Cell Division , Chaperonin 60/biosynthesis , Microtubules/ultrastructure , Mitochondria/ultrastructure , Mutation , Proton-Translocating ATPases/biosynthesis , Schizosaccharomyces/cytology , Schizosaccharomyces/genetics , Tubulin/biosynthesisABSTRACT
Yeast cells with mutations in BRO1 display phenotypes similar to those caused by deletion of BCK1, a gene encoding a MEK kinase that functions in a mitogen-activated protein kinase pathway mediating maintenance of cell integrity. bro1 cells exhibit a temperature-sensitive growth defect that is suppressed by the addition of osmotic stabilizers or Ca2+ to the growth medium or by additional copies of the BCK1 gene. At permissive temperatures, bro1 mutants are sensitive to caffeine and respond abnormally to nutrient limitation. A null mutation in BRO1 is synthetically lethal with null mutations in BCK1, MPK1, which encodes a mitogen-activated protein kinase that functions downstream of Bck1p, or PKC1, a gene encoding a protein kinase C homolog that activates Bck1p. Analysis of the isolated BRO1 gene revealed that it encodes a novel, 97-kDa polypeptide which contains a putative SH3 domain-binding motif and is homologous to a protein of unknown function in Caenorhabditis elegans.
Subject(s)
Calcium-Calmodulin-Dependent Protein Kinases/metabolism , Genes, Fungal , Protein Kinase C/metabolism , Saccharomyces cerevisiae/enzymology , Saccharomyces cerevisiae/genetics , Amino Acid Sequence , Animals , Base Sequence , Caenorhabditis elegans/genetics , Calcium/pharmacology , Calcium-Calmodulin-Dependent Protein Kinases/genetics , Cell Division/drug effects , Cloning, Molecular , DNA, Fungal/genetics , Helminth Proteins/genetics , Molecular Sequence Data , Mutation , Phenotype , Protein Kinase C/genetics , Restriction Mapping , Saccharomyces cerevisiae/growth & development , Sequence Homology, Amino AcidSubject(s)
Mitochondria/physiology , Mutation , Saccharomyces cerevisiae/genetics , Cell Division/genetics , Genetic Techniques , Microbiological Techniques , Microscopy/methods , Microscopy, Electron/methods , Mitochondria/ultrastructure , Saccharomyces cerevisiae/cytology , Saccharomyces cerevisiae/isolation & purificationABSTRACT
Yeast cells with the mdm10 mutation possess giant spherical mitochondria and are defective for mitochondrial inheritance. The giant mitochondria display classical features of mitochondrial ultrastructure, yet they appear incapable of movement or division. Genetic analysis indicated that the mutant phenotypes resulted from a single nuclear mutation, and the isolated MDM10 gene restored wild-type mitochondrial distribution and morphology when introduced into mutant cells. MDM10 encodes a protein of 56.2 kD located in the mitochondrial outer membrane. Depletion of Mdm10p from cells led to a condensation of normally extended, tubular mitochondria into giant spheres, and reexpression of the protein resulted in a rapid restoration of normal mitochondrial morphology. These results demonstrate that Mdm10p can control mitochondrial morphology, and that it plays a role in the inheritance of mitochondria.
Subject(s)
Fungal Proteins/physiology , Intracellular Membranes/chemistry , Membrane Proteins/physiology , Mitochondria/ultrastructure , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/ultrastructure , Amino Acid Sequence , Base Sequence , Fungal Proteins/genetics , Membrane Proteins/genetics , Mitochondria/chemistry , Molecular Sequence Data , Mutation , Saccharomyces cerevisiae/geneticsABSTRACT
Intermediate filaments are abundant cytoskeletal components whose specific cellular functions are poorly understood. The Saccharomyces cerevisiae protein MDM1 displays structure and solubility properties that are similar to those of intermediate filament proteins of animal cells. Yeast cells that have a mutant form of MDM1 exhibit temperature-sensitive growth and defective transfer of nuclei and mitochondria to daughter cells during incubation at the nonpermissive temperature of 37 degrees C. The purified, wild-type MDM1 protein readily forms 10-nanometer-wide filaments at either 4 degrees C or 37 degrees C. In contrast, the purified, mutant protein forms filaments at 4 degrees C but fails to form such structures at 37 degrees C. These results suggest that intermediate filament proteins are universal components of eukaryotic cells.
Subject(s)
Cell Cycle Proteins , Fungal Proteins/metabolism , Intermediate Filaments/metabolism , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/metabolism , Cell Division , Cell Nucleus/metabolism , Fungal Proteins/genetics , Genes, Fungal , Intermediate Filament Proteins , Intermediate Filaments/ultrastructure , Microscopy, Electron , Mitochondria/metabolism , Mutation , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/ultrastructure , TemperatureABSTRACT
The mdml mutation causes temperature-sensitive growth and defective transfer of nuclei and mitochondria into developing buds of yeast cells at the nonpermissive temperature. The MDM1 gene was cloned by complementation, and its sequence revealed an open reading frame encoding a potential protein product of 51.5 kD. This protein displays amino acid sequence similarities to hamster vimentin and mouse epidermal keratin. Gene disruption demonstrated that MDM1 is essential for mitotic growth. Antibodies against the MDM1 protein recognized a 51-kD polypeptide that was localized by indirect immunofluorescence to a novel pattern of spots and punctate arrays distributed throughout the yeast cell cytoplasm. These structures disappeared after shifting mdm1 mutant cells to the nonpermissive temperature, although the cellular level of MDM1 protein was unchanged. Affinity-purified antibodies against MDM1 also specifically recognized intermediate filaments by indirect immunofluorescence of animal cells. These results suggest that novel cytoplasmic structures containing the MDM1 protein mediate organelle inheritance in yeast.
Subject(s)
Cell Cycle Proteins , Cell Nucleus/physiology , Fungal Proteins/genetics , Genes, Fungal , Mitochondria/physiology , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Amino Acid Sequence , Animals , Base Sequence , DNA, Fungal/genetics , DNA, Fungal/isolation & purification , Fluorescent Antibody Technique , Fungal Proteins/analysis , Genetic Complementation Test , Intermediate Filament Proteins , Keratins/genetics , Molecular Sequence Data , Molecular Weight , Nocodazole/pharmacology , Open Reading Frames , Plasmids , Restriction Mapping , Saccharomyces cerevisiae/drug effects , Saccharomyces cerevisiae/ultrastructure , Sequence Homology, Nucleic Acid , Vimentin/geneticsABSTRACT
The nuclear mas5 mutation causes temperature-sensitive growth and defects in mitochondrial protein import at the nonpermissive temperature in the yeast Saccharomyces cerevisiae. The MAS5 gene was isolated by complementation of the mutant phenotypes, and integrative transformation demonstrated that the complementing fragment encoded the authentic MAS5 gene. The deduced protein sequence of the cloned gene revealed a polypeptide of 410 amino acids which is homologous to Escherichia coli DnaJ and the yeast DnaJ log SCJ1. Northern (RNA blot) analysis revealed that MAS5 is a heat shock gene whose expression increases moderately at elevated temperatures. Cells with a deletion mutation in MAS5 grew slowly at 23 degrees C and were inviable at 37 degrees C, demonstrating that MAS5 is essential for growth at increased temperatures. The deletion mutant also displayed a modest import defect at 23 degrees C and a substantial import defect at 37 degrees C. These results indicate a role for a DnaJ cognate protein in mitochondrial protein import.