ABSTRACT
A comparative analysis of the effect of the five acridine, three phenothiazine and one xanten (pyronine G) derivatives on the activity of liver mitochondrial monoamine oxidase (MAO) in sexually mature male river lampreys Lampetra fluviatilis has been conducted. Tyramine, dopamine, serotonin, noradrenaline, benzylamine, ß-phenylethylamine and N-methylhistamine have been used as substrates for analyzing the monoamine oxidase activity of heterocyclic compounds. The analyzed synthetic hexamerous tricyclic compounds exhibit irreversible inhibition of the enzyme but no specificity depending on the desaminated substrate. The number and identity of heteroatoms in the analyzed heterocyclic compounds have been established to influence their inhibitory efficiency. The data of substrate-inhibitory analysis obtained with the use of the specific substrates provide indirect evidence for the existence of a single MAO form in the lamprey liver.
Subject(s)
Heterocyclic Compounds/pharmacology , Liver/enzymology , Monoamine Oxidase Inhibitors/pharmacology , Monoamine Oxidase/metabolism , Animals , Biogenic Monoamines/metabolism , Heterocyclic Compounds/chemistry , Lampreys , Liver/metabolism , Male , Monoamine Oxidase Inhibitors/chemistryABSTRACT
The substrate and inhibitory specificity of mitochondrial monoamine oxidase (MAO) in the liver of males of the summer form of the chum salmon Oncorhynchus keta was studied. As to the spectrum of deaminated substrates, the hepatic MAO of the chum salmon is similar to MAO of most terrestrial mammals, for eight classical MAO substrates similarity in their substrate characteristics were found. Analysis of the antimonoamine oxidase activity of two derivaties of 2-propinilamine, five derivatives of acridine as well as of pyronine G revealed significant qualitative and quantitative differences as compared to the hepatic enzyme of tuna and whitefish. The compounds tested manifested themselves as irreversible inhibitors of chum salmon's hepatic MAO possessing various efficacy, but lacking the selectivity of action as dependent on the deaminated substrate. The obtained data on the substrate and inhibitory analysis provide an indirect evidence for the presence of a single molecular form of MAO in the chum salmon liver.
Subject(s)
Fish Proteins/metabolism , Liver/enzymology , Mitochondrial Proteins/metabolism , Monoamine Oxidase/metabolism , Salmon/metabolism , Animals , Enzyme Inhibitors/pharmacology , Mitochondrial Proteins/antagonists & inhibitors , Pargyline/analogs & derivatives , Pargyline/pharmacology , Propylamines/pharmacology , Pyronine/pharmacology , Salmonidae/metabolism , Species Specificity , Tuna/metabolismABSTRACT
There has been carried out a study of substrate and inhibitor specificity of the liver mitochondrial monoamine oxidase (MAO) of the striped-bellied tunny Katsuwonus pelamis. Results of the substrate-inhibitor analysis with use of chlorgilin and deprenyl are an indirect proof for the existence in the tunny liver of one molecular MAO form. The studied enzyme, like the liver MAO of terrestrial mammals, deaminates tyramine, tryptamine, dopamine, serotonin, noradrenalin, benzylamine, ß-phenylethylamine, and N-methylhistamine, does not deaminate histamine and is not inhibited by 10 mM semicarbaside. Tacrine, acriflavine, proflavine, acridine orange, and pyronin G have been established to be irreversible inhibitors of intermediate strength with respect to the tunny liver MAO. Specificity of action of the inhibitors at deamination of different substrates was identical.
Subject(s)
Fish Proteins/metabolism , Liver/enzymology , Mitochondria, Liver/enzymology , Monoamine Oxidase/metabolism , Tuna/metabolism , Animals , Biogenic Monoamines/metabolism , Mitochondria, Liver/drug effects , Monoamine Oxidase Inhibitors/pharmacology , Substrate SpecificitySubject(s)
Amine Oxidase (Copper-Containing)/metabolism , Hepatopancreas/enzymology , Monoamine Oxidase/metabolism , Octopodiformes/enzymology , Amine Oxidase (Copper-Containing)/antagonists & inhibitors , Animals , Hepatopancreas/cytology , Mitochondria/enzymology , Monoamine Oxidase Inhibitors/pharmacology , Octopodiformes/cytology , Substrate SpecificitySubject(s)
Liver/enzymology , Mitochondrial Proteins/chemistry , Phoca/metabolism , Animals , Enzyme Activation , Enzyme StabilityABSTRACT
Results obtained from complex medical-physiological investigations performed during 10 scientific expeditions in the Arkhangel'sk Region in 2003-2005 are presented. The effects of climatological-geographic, biogeochemical, and social conditions of the conditions obtaining in the Far North region of Russia on sexual maturation, formation of the structural-functional organization of the brain, autonomic functions, and immunological and biochemical status of schoolchildren were studied using state-of-the-art neurophysiological methods (computerized electroencephalography, computerized rheoencephalography, computerized electric dipole origin tomography, etc.), psychophysiological and psychometric methods (assessment of the state of cognitive and memory functions, Wechsler intellectual scale), along with biochemical assay of monoamine oxidase (MAO, the key enzyme in adrenergic neurotransmitter metabolism) and the liver enzyme butyrylcholinesterase (BuChE) and physicochemical analysis of the levels of macroelements and trace elements in the body.
