Si-PTN inhibits the growth of hypertrophic scar fibroblasts / 中华整形外科杂志

Objective@#To analyze the effects of PTN in the growth of hypertrophic scar fibroblasts.@*Methods@#The primary scar fibroblasts were extracted from fresh hypertrophic scar tissues of 18 patients, to restrict the si-PTN cell line. After successful construction, the scar fibroblasts in logarithmic growth were divided into si-PTN group and control group, transfected with 2 μg si-PTN and control nonsense chains, respectively. MTT, PI staining and Annexin V-FITC/PI assay were used to detect the proliferation and apoptosis of si-PTN and control groups. Western blot and RT-PCR were used to detect the expression level of Cyclin D1, Bcl-2 and Bax in si-PTN and control groups.@*Results@#The inhibition rates of si-PTN on the proliferation of scar fibroblasts were 14.49%, 13.24%, 20.78% and 23.12% at 1, 2, 3 and 4 days, respectively. The difference was statistically significant compared with the control group (P<0.05). The results of cell cycle experiments showed that the proportion of G0-G1 phase in the si-PTN group was (60.79±3.34)%, which was higher than that of the control group (50.54±1.80)% (P=0.02). The proportion of S phase cells in the si-PTN group was (29.02±2.07)%, which was lower than that in the control group (40.08±2.89)% (P=0.03). The results of apoptosis assay showed that the proportion of early and late apoptotic cells in si-PTN group were (8.17±0.57)% and (6.80±0.74)%, respectively, which were higher than that of the control group (4.35±0.46)%, and (4.70±0.48)% (P<0.05). Western Blot and RT-PCR results showed that compared with the control group, the si-PTN group was down-regulated by Cyclin D1 (43.76%) and Bcl-2 (43.56%), Bax was up-regulated (31.97%) (P<0.05).@*Conclusions@#si-PTN inhibited the growth of scar fibroblasts.

Clinical application and effects of two different liposuction methods in breast augmentation by autologous fat grafting / 中华医学美学美容杂志

Objective To explore the effect of different harvest methods of liposuction on the autologous fat grafting.Methods The clinical data of 60 patients with fat grafting for breast augmentation from January 2014 to January 2017 were analyzed retrospectively.According to the method of liposuction,60 patients were divided into the water-jet assisted liposuction group (30 cases) and negative-pressure machine liposuction group (30 cases).The surgical time of different liposuctions and the fat survival were compared after breast augmentation.The clinical effect of different liposuction methods was analyzed by follow-up one year after operation.Results Sixty patients completed the surgery.The fat survival rate of water-jet assisted liposuction was (66.71±2.68) ％,and the fat survival rate of the negative-pressure machine liposuction was (51.44 ± 1.16) ％.There were statistically significant difference between the two groups (P＜0.05).As for the operation time of liposuction,the water-jet assisted liposuction group was (33.28 ± 2.96) min,the negative-pressure machine liposuction group was (52.91± 5.03) min;there were statistically significant difference between the two groups (P ＜ 0.05).There was no statistically significant difference in satisfactory rate (P＞0.05).Conclusions Compared with negative pressure liposuction,water-jet assisted liposuction using autologous fat grafting in breast augmentation can improve the survival rate of fat transplantation.This technique has good clinical application value.

Effects of Dietary Selenium Supplementation on Seminiferous Tubules and SelW, GPx4, LHCGR, and ACE Expression in Chicken Testis.

We investigated the effects of dietary selenium (Se) supplementation on the development of chicken testis and the expression of selenoprotein W (SelW), glutathione peroxidase4 (GPx4), luteinizing hormone/choriogonadotropin receptor (LHCGR), and angiotensin converting enzyme (ACE). Sixty roosters were assigned randomly into the control group fed with a basic diet (containing 0.3 mg Se/kg) and the experimental group fed with a diet (containing 0.6 mg Se/kg). The testes were collected individually at age of 6, 9, and 12 weeks. Se was supplemented in chicken feed for 15 days before sampling. The results indicated that dietary Se affected the number of cells in the seminiferous tubules and viability of Sertoli cells in vitro culture. SelW and GPx4 expression in the testes increased significantly in the experimental group compared to that in the control group. LHCGR expression in the testes increased significantly in the experimental group after 12 weeks compared to that in the control group. In contrast, ACE expression was inhibited in the experimental group compared to that in the control group. These results suggest that dietary supplementation with Se improved development of the seminiferous tubules at the cellular level and that SelW, GPx4, LHCGR, and ACE are involved.

The effects of sonicated extract of Porphyromonas endodontalis on the cell cycle and apoptosis of osteo-blasts / 实用口腔医学杂志

Objective:To observe the effects of sonicated extract of Porphyromonas endodontalis(P.endodontalis)on the cell cy-cle and apoptosis of human osteoblastic hFOB 1 .1 9 cells.Methods:hFOB 1 .1 9 cells were treated with sonicated extract of P.end-odontalis at 0 (the control),1 ,1 0 and 1 00 μg/ml for 1 2,24 and 48 h respectively.MTT assay was used to examine cell prolifera-tion.The cell cycle distribution and apoptosis were examined by flow cytometry.Results:The sonicated exact of P.endodontalis in-hibited the proliferation of hFOB 1 .1 9 cells in a time-and dose-dependent manner.24 h treatment of 1 0 μg/ml and 1 00 μg/ml ex-act arrested the cell cycle of the cells in G1 phase,48 h treatment induced apoptosis in a dose-dependent manner.Conclusion:Sonicated exact of P.endodontalis may arrest hFOB 1 .1 9 cells in G1 phase and induce cell apoptosis,leading to inhibition of the cell proliferation.