ABSTRACT
OBJECTIVE: To study the histomorphometrical characteristics of lymphatic vessels in cavernous lymphangiomas of the tongue. MATERIAL AND METHODS: Immunohistochemical stainings were prepared in the 20 specimens with three antibodies [D2-40, CD31 and proliferating cell nuclear antigen (PCNA)]. Three-dimensional (3D) reconstruction and histometrical analysis of the lymphatic vessels was also examined. RESULTS: Distinctly positive staining for D2-40 was found in dilated lymphatic vessels located in the lamina propria beneath the thinned covering epithelium. Small blood vessels stained positively for CD31 were present in the lamina propria. PCNA-positive lymphatic endothelial cells were scattered in both control and lymphangioma. The 3D architecture of lymphatic vessels was characterized by a complex network with irregular branches in the lamina propria. Histometrical analysis showed that the number of lymphatic endothelial cells per lymphatic vessel perimeter in cavernous lymphangioma was significantly higher than that in control. There were no significant differences in the lymphatic density and the ratio of PCNA-positive lymphatic endothelial cell nuclei to the total number of lymphatic endothelial cell nuclei between control and lymphangioma. CONCLUSIONS: These results indicate the absence of excessive endothelial cell proliferation in dilated lymphatic vessels in cavernous lymphangioma. Cavernous lymphangioma may be attributed to the enlargement of lymphatic vessels without the tumorous proliferation of lymphatic endothelial cells.
Subject(s)
Lymphangioma/pathology , Tongue Neoplasms/pathology , Adolescent , Adult , Aged , Antibodies, Monoclonal/analysis , Antibodies, Monoclonal, Murine-Derived , Biomarkers, Tumor/analysis , Cell Nucleus/ultrastructure , Child , Child, Preschool , Endothelial Cells/pathology , Endothelium, Lymphatic/pathology , Epithelium/pathology , Female , Humans , Image Processing, Computer-Assisted/methods , Imaging, Three-Dimensional/methods , Immunohistochemistry , Lymphatic Vessels/pathology , Male , Microcirculation/pathology , Middle Aged , Mucous Membrane/pathology , Platelet Endothelial Cell Adhesion Molecule-1/analysis , Proliferating Cell Nuclear Antigen/analysisABSTRACT
The 6-methyl-2-(2-pyridyl)-benzimidazole (biz) ligand coordinates with the actinide species in solution, and the complexes display efficient intramolecular energy-transfer processes. The energy transfer in the Cm(III)-biz system proceeds in a nonradiative mode, whereas a radiative mode is the principal mechanism in the Am(III)-biz system.
ABSTRACT
The local structures of Am, Nd and Er-Benzimidazole (Biz) in solution were determined by EXAFS. The BIZ molecule coordinated to Am and Nd through two nitrogen atoms in a bidentate fashion. Two nitrogen atoms of BIZ ligated to Am and Nd with the bond distances R(Am-n) N=2.63A and R(Nd-N) = 2.65 A, respectively. The total coordination number of the Am BIZ complexes (at a molar ratio of metal ion to ligand of 1:20) was approximately 10 but that of Nd BIZ complex was approximately 9.
ABSTRACT
The local structure of the trivalent lanthanide (Ln(III)) complexes with oxydiacetic acid (ODA) and iminodiacetic acid (IDA) in aqueous solution was investigated by EXAFS spectroscopy. The coordination number and the bond distance were obtained by the detailed EXAFS analysis. The coordination number of Ln(III) in both the Ln-ODA and -IDA systems decreases from nine for lighter Ln(III) to eight for heavier Ln(III). The bond distances of ether O atoms from Ln(III) in the Ln(ODA)(3)3- complexes are shorter than those of N atoms in the Ln(IDA)(3)3- ones.
