ABSTRACT
C57Bl/6J mice were exposed to γ-radiation in a dose of 7.5 Gy. A week later, the experimental group received intravenous injection of extracellular vesicles isolated from the culture medium of human bone marrow multipotent mesenchymal stromal cells. Changes in the physiological parameters of animals were assessed by laser correlation spectroscopy, histological examination, cytometry, and by differential leukocyte count. In 3 and 6 weeks, the parameters of the experimental group occupied an intermediate position between the intact and irradiated groups or did not differ significantly from the parameters of the intact group. The rate and efficiency of recovery varied at different levels of organization. Reduction of damage caused by irradiation in a sublethal dose at different levels of organization of experimental animals was shown.
Subject(s)
Extracellular Vesicles/metabolism , Gamma Rays , Mesenchymal Stem Cells/metabolism , Mesenchymal Stem Cells/radiation effects , Animals , Body Weight/radiation effects , Bone Marrow Cells/cytology , Bone Marrow Cells/radiation effects , Cells, Cultured , Humans , Male , Mice , Mice, Inbred C57BLABSTRACT
BACKGROUND: The high level of physical activity is known to contribute to the harmonious development of children and adolescents: moreover; it is a factor promoting prevention of hypodynamia. The low level of physical activity is a risk factor for the development of many non-communicable diseases. However, physical education is not considered by many students as an attractive subject. AIM: The objective of the present study was the enhancement of the motivational aspects of physical activity for the prevention of hypodynamia among students. MATERIAL AND METHODS: Physical education of the students was carried out with the use of the standard and modified methods. A total of 206 students (including 133 girls and 73 boys) at the mean age of 17.5±1.5 years were available for the examination. The study was conducted before the intervention (1st year) after the termination of the physical culture lessons envisaged by the academic curriculum (3rd year). The evaluation of the physical conditions and fitness of the students was conducted on the basis of the Russian Physical Culture and Sport Complex (GTO), bioimpedance analysis making use of the 'Medass' ABC-01 device; the functional reserves of the organism were assessed by means of hardware-software programs; the changes in food preferences were estimated on the basis of the FLLANK, questionnaire preliminarily adapted to the specific conditions of this country, and the characteristics of the metabolic processes were evaluated by laser correlation spectroscopy. RESULTS: Dynamics of the successful performance of the GTO normatives in the girls manifested itself in the in reduction of time they spent to run the 100 meter distance; the same was true for the boys who had to overcome the distance of 3000 meters. The students of both sexes became capable of increasing the number of repetitions of crunch and jumping rope exercises. The basal metabolism in the girls was increased within one year after the start of classes, and in the boys two years after the onset of the exercises; simultaneously, the positive changes in the metabolic profile were documented in the students of either sex. No adverse events related to the application of the modified training methods were recorded. CONCLUSION: An important aspect of the present study was the impact of new methods of teaching physical education on the composite body composition. The modified methods were found to shift dietary preferences and metabolic processes in the body of the students after the termination of the educational program.
Subject(s)
Physical Education and Training/methods , Students/psychology , Students/statistics & numerical data , Adolescent , Anthropometry , Basal Metabolism , Female , Food Preferences , Humans , Male , Physical Fitness , Program Evaluation , Russia , Young AdultABSTRACT
AIM: An aim of the study was to compare informative value of traditional approach (anti-AChR antibody radioimmunoassay) and evaluation of metabolic shifts by laser correlation spectroscopy in myasthenia gravis. MATERIAL AND METHODS: The search for the relationship between the disease severity in 77 patients, 12-80 years and the distribution pattern of subfraction serum components revealed three informative zones: 6-15, 27-67, and 127-223 nm. RESULTS AND CONCLUSION: In patients without disturbances of vital functions, the contribution of the first zone particles into light scatter increases and that of the third zone particles decreases. Considerable differences attaining the level of statistical significance in zones 6 and 20 nm were revealed in the spectra of serum from patients with myasthenia gravis of the same severity with and without thymoma. This opens prospects for dynamic monitoring of the efficiency of therapy.
Subject(s)
Autoantibodies/blood , Myasthenia Gravis/immunology , Receptors, Cholinergic/immunology , Drug Monitoring/methods , Female , Humans , Lasers , Myasthenia Gravis/blood , Myasthenia Gravis/diagnosis , Myasthenia Gravis/drug therapy , Radioimmunoassay , Spectrum Analysis , Thymoma/bloodABSTRACT
The article considers interaction of nano-particles of titan oxide and blood serum of patients with verified diagnoses of "myasthenia" and "allergy". The analysis of solution of nano-powder of titan oxide using technique of laser correlated spectrometry demonstrated that main input into light scattering is made by particles with radius of 4.64-8.42 nm. The data concerning dimensions of particles received with laser correlated spectrometry matches data of analysis of these particles with various physical techniques. The nano-particles of titan oxide stabilized with phosphoric acid at interaction with blood serum cause development and sedimentation of complexes with albumins and globulins. The interaction of nano-particles of titan oxide with blood serum of patients with myasthenia results in elimination from it the significant percentage (40%) of circulating immune complexes. In blood serum of patients with bronchial asthma, nano-particles of titan oxide bind proteins of small size mainly.
