Correlative Fluorescent Scanning Probe Nanotomography Used to Study the Intracellular Distribution of Doxorubicin in MCF-7 Human Breast Adenocarcinoma Cells.

Developing technologies for efficient targeted drug delivery for oncotherapy requires new methods to analyze the features of micro- and nanoscale distributions of antitumor drugs in cells and tissues. A new approach to three-dimensional analysis of the intracellular distribution of cytostatics was developed using fluorescence scanning optical-probe nanotomography. A correlative analysis of the nanostructure and distribution of injected doxorubicin in MCF-7 human breast adenocarcinoma cells revealed the features of drug penetration and accumulation in the cell. The technology is based on the principles of scanning optical probe nanotomography and is applicable to studying the distribution patterns of various fluorescent or fluorescence-labelled substances in cells and tissues.

Threefold Increase of the Bulk Electron Temperature of Plasma Discharges in a Magnetic Mirror Device.

This Letter describes plasma discharges with a high temperature of bulk electrons in the axially symmetric high-mirror-ratio (R=35) open magnetic system gas dynamic trap (GDT) in the Budker Institute (Novosibirsk). According to Thomson scattering measurements, the on-axis electron temperature averaged over a number of sequential shots is 660±50 eV with the plasma density being 0.7×10^{19} m^{-3}; in few shots, electron temperature exceeds 900 eV. This corresponds to at least a threefold increase with respect to previous experiments both at GDT and at other comparable machines, thus, demonstrating the highest quasistationary (about 1 ms) electron temperature achieved in open traps. The breakthrough is made possible by application of a new 0.7 MW/54.5 GHz electron cyclotron resonance heating system in addition to standard 5 MW heating by neutral beams, and application of a radial electric field to mitigate the flute instability.

Progress in Mirror-Based Fusion Neutron Source Development.

The Budker Institute of Nuclear Physics in worldwide collaboration has developed a project of a 14 MeV neutron source for fusion material studies and other applications. The projected neutron source of the plasma type is based on the gas dynamic trap (GDT), which is a special magnetic mirror system for plasma confinement. Essential progress in plasma parameters has been achieved in recent experiments at the GDT facility in the Budker Institute, which is a hydrogen (deuterium) prototype of the source. Stable confinement of hot-ion plasmas with the relative pressure exceeding 0.5 was demonstrated. The electron temperature was increased up to 0.9 keV in the regime with additional electron cyclotron resonance heating (ECRH) of a moderate power. These parameters are the record for axisymmetric open mirror traps. These achievements elevate the projects of a GDT-based neutron source on a higher level of competitive ability and make it possible to construct a source with parameters suitable for materials testing today. The paper presents the progress in experimental studies and numerical simulations of the mirror-based fusion neutron source and its possible applications including a fusion material test facility and a fusion-fission hybrid system.