Association of HOTAIR rs1899663 G>T Polymorphism with Colorectal Cancer in the Turkish Population: A Case-Control Study.

BACKGROUND: Long noncoding RNAs increase the overexpression of oncogenes. Cancer development and metastasis of cancer may occur as a result of overexpression of oncogenes. Polymorphisms in the genes (such as HOTAIR, etc.) in which long noncoding RNAs are synthesized affect the expression of these genes. Therefore, these polymorphisms may play a role in cancer development and cancer metastasis. The present study aimed to evaluate the association between HOTAIR gene rs1899663 G>T polymorphism with colorectal cancer. METHODS: The current study examined the HOTAIR gene rs1899663 G>T polymorphism in 100 patients with colorectal cancer and 93 healthy persons by a real-time polymerase chain reaction. RESULTS: The G and T allele frequencies of the HOTAIR rs1899663 polymorphism were significantly different between the case and control groups (P = .01). The persons carrying the G allele had a protective effect against colorectal cancer, while individuals carrying the T allele were predisposed to colorectal cancer (P = .001). Four of 5 colorectal cancer recurrence patients had the TT genotype (P = .02). CONCLUSION: This research is the first to demonstrate the relationship between colorectal cancer and the HOTAIR gene rs1899663 polymorphism in the Turkish population, which is a Caucasian population. Our results suggest that the rs1899663 G allele has a protective role for colorectal cancer in the Turkish population. However, it would be appropriate to conduct this research with a larger sample to confirm this result in the Turkish population.

Evaluation of possible role of the hTERT gene rs2853669 polymorphism in the development of colorectal cancer as a genetic risk factor.

Colorectal cancer (CRC) is the second deadliest malignancy. Human telomerase reverse transcriptase (hTERT) gene has been identified as one of the potential cancer susceptibility genes. We evaluated the relationship between the risk of CRC and CRC's clinicopathological features of the hTERT rs2853669 (A > G/T > C, by the chain direction) polymorphism in Turkish population. The rs2853669 polymorphism was investigated with the LightCycler 96 device in 100 CRC patients and 327 controls. We found that the rs2853669 polymorphism AG/GG genotypes in genetic models reduced the risk of CRC. However, there was no significant relationship between rs2853669 polymorphism and clinicopathological features of CRC in studied population. The results of this study showed that the risk of colorectal cancer is significantly reduced in the individuals having the G (C) allele. Our recommendation is to analyze the hTERT gene expression by studying the hTERT promoter mutations with this polymorphism in colorectal cancer.

Relationship between IL28B gene rs8099917 polymorphism and SVR in Turkish patients with hepatitis C virus genotype 1.

BACKGROUND AND OBJECTIVE: The hepatitis C virus (HCV) which infects 3% of the world's population is a global challenge. Recently, genome-wide association studies (GWAS) have identified that the IL28B gene rs8099917 polymorphism was associated with the response to the pegylated-interferon alpha/ribavirin (PegIFNα/RBV) combination therapy in patients infected with HCV genotype 1. IL28B gene rs8099917 polymorphism should be determined before beginning treatment of HCV-infected patients to predict an individual's response. The aims of this study were to analyze the correlation between IL28B gene rs8099917 (T/G) polymorphism and PegIFNα/RBV therapy outcome in the Turkish population. METHODS: Genotypes of the IL28B gene rs8099917 (T/G) single nucleotide polymorphism (SNP) were determined in 308 patients with HCV infection by using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. One group consisted of 148 patients with a sustained virological response (SVR), whereas the second group consisted of 160 nonresponders (non-SVR). RESULTS: Allele and genotype associations of IL28B gene rs8099917 polymorphism with a sustained virological response were observed in comparisons between the SVR and non-SVR groups (P<0.001). In addition, the characteristics of the subjects did not differ between these two groups except for age and fibrosis stage (P<0.05). Additionally, neither SVR nor rs80999917 genotypes were associated by HCV RNA levels. CONCLUSIONS: In conclusion, the rs8099917 polymorphism was thus found strongly associated with a sustained virological response to therapy in Turkish patients infected with HCV genotype 1. Consequently, we suggest determining IL28B gene rs8099917 polymorphism of patients with HCV genotype 1 before onset of treatment.