A unique case in which Kimoto-style fermentation was completed with Leuconostoc as the dominant genus without transitioning to Lactobacillus.

The Kimoto-style fermentation starter is a traditional preparation method of sake brewing. In this process, specific microbial transition patterns have been observed within nitrate-reducing bacteria and lactic acid bacteria during the production process of the fermentation starter. We have characterized phylogenetic compositions and diversity of the bacterial community in a sake brewery performing the Kimoto-style fermentation. Comparing the time-series changes with other sake breweries previously reported, we found a novel type of Kimoto-style fermentation in which the microbial transition differed significantly from other breweries during the fermentation step. Specifically, the lactic acid bacteria, Leuconostoc spp. was a predominant species in the late stage in the preparation process of fermentation starter, on the other hand, Lactobacillus spp., which plays a pivotal role in other breweries, was not detected in this analysis. The discovery of this new variation of microbiome transition in Kimoto-style fermentation has further deepened our understanding of the diversity of sake brewing.

Independently programmable frequency-multiplexed phase-sensitive optical parametric amplification in the optical telecommunication band.

We experimentally demonstrate programmable multimode phase-sensitive amplification multiplexed in the frequency domain for flexible control of parallelly generated squeezed states. We utilize the unique phase-matching condition of a type-II periodically poled potassium titanyl phosphate (PPKTP) crystal and pulse shaping technique to fully control the frequency-domain parallel generation of squeezed states in the optical telecommunication band. We experimentally verify that the independent programmability of phase-sensitive optical parametric amplification (OPA) for the modes corresponding to different frequency bands can be achieved by shaping the pump laser pulse from optical parametric gain measurements using a coherent probe light generated by a degenerate synchronously pumped optical parametric oscillator.