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1.
Biochem Biophys Res Commun ; 678: 193-199, 2023 10 20.
Article in English | MEDLINE | ID: mdl-37651888

ABSTRACT

Severely immunodeficient mice are useful for understanding the pathogenesis of certain tumors and for developing therapeutic agents for such tumors. In addition, engraftment of these mice with human hematopoietic cells can yield information that helps us understand the in vivo molecular mechanisms underlying actual human viral infections. In our present research, we discovered a novel, severely immunodeficient strain of mice having a mutation in exon 57 of the Prkdc gene (PrkdcΔex57/Δex57) in an inbred colony of B10.S/SgSlc mice. Those PrkdcΔex57/Δex57 mice showed thymic hypoplasia and lack of mature T cells and B cells in peripheral lymphoid tissues, resulting in very low levels of production of serum immunoglobulins. In addition, those mice were highly susceptible to influenza viruses due to the lack of acquired immune cells. On the other hand, since they had sufficient numbers of NK cells, they rejected tumor transplants, similarly to Prkdc+/+ mice. Next, we generated Foxn1nu/nuPrkdcΔex57/Δex57Il2rg-/- (NPG) mice on the BALB/cSlc background, which lack all lymphocytes such as T cells, B cells and innate lymphoid cells, including NK cells. As expected, these mice were able to undergo engraftment of human tumor cell lines. These findings suggest that PrkdcΔex57/Δex57 mice will be useful as a novel model of immunodeficiency, while NPG mice will be useful for xenografting of various malignancies.


Subject(s)
Immunity, Innate , Immunologic Deficiency Syndromes , Humans , Animals , Mice , Killer Cells, Natural , B-Lymphocytes , Cell Line, Tumor , DNA-Binding Proteins , DNA-Activated Protein Kinase
2.
Microbiol Immunol ; 67(3): 105-113, 2023 Mar.
Article in English | MEDLINE | ID: mdl-36604771

ABSTRACT

The bone marrow (BM) stromal cell antigen-2 (BST-2), also known as tetherin, CD317, PDCA-1, or HM1.24, is a membrane protein overexpressed in several types of tumors and may act as a promising target for cancer treatment via antibody-dependent cellular cytotoxicity. BST-2 is also expressed in human BM stromal cells (BMSC), which support B cell development. While the activity of BST-2 as an antiviral factor has been demonstrated, the expression patterns and the role of BST-2 on B-cell development and activation have not been investigated, especially in vivo. In this study, Bst2 knockout (Bst2-/- ) mice were generated to assess the role of BST-2 on B cell development and activation. It was observed that BST-2 was not expressed in BMSC or all B cell progenitors even in wild-type mice and does not play a significant role in B cell development. In addition, the loss of BST-2 had no effect on B cell activation. Furthermore and in contrast to the well-known antiviral role of BST-2, infection of vesicular stomatitis Indiana virus to the BM cells collected from the Bst2-/- mice produced less infectious virus compared with that from the WT mice. These results suggest that murine BST-2 is different from human BST-2 in the expression pattern, physiological function, in vivo, and might possess positive role on VSV replication.


Subject(s)
Bone Marrow Stromal Antigen 2 , Animals , Humans , Mice , Membrane Proteins , Vesicular stomatitis Indiana virus , Bone Marrow Stromal Antigen 2/metabolism
3.
Biochem Biophys Res Commun ; 628: 57-63, 2022 11 05.
Article in English | MEDLINE | ID: mdl-36081279

ABSTRACT

Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by type 2 immune responses. Interleukin-25 (IL-25) is produced predominantly by epithelial cells. It can activate Th2 cells to produce type 2 cytokines such as IL-4, IL-5 and IL-13, contributing to host defense against nematodes. However, excessive/inappropriate production of IL-25 is considered to be involved in development of type 2 cytokine-associated allergic disorders such as asthma. On the other hand, the contribution of IL-25 to the pathogenesis of AD remains poorly understood. In the present study, we found that expression of Il25 mRNA was significantly increased in the skin of mice during oxazolone-induced chronic contact hypersensitivity (CHS), which is a mouse model of human AD. In addition, development of oxazolone-induced chronic CHS was significantly reduced in IL-25-deficient (Il25-/-) mice compared with wild-type mice on the C57BL/6, but not BALB/c, background, although IL-25 was not essential for IL-4 production by hapten-specific T cells. Therefore, IL-25 is crucial for development of chronic CHS, although that is partly dependent on the genetic background of the mice.


Subject(s)
Dermatitis, Atopic , Dermatitis, Contact , Interleukin-17 , Animals , Cytokines/metabolism , Dermatitis, Atopic/chemically induced , Dermatitis, Atopic/genetics , Dermatitis, Contact/genetics , Haptens , Interleukin-13 , Interleukin-17/genetics , Interleukin-4/genetics , Interleukin-5 , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Oxazolone , RNA, Messenger , Skin/metabolism
4.
Sci Rep ; 11(1): 5913, 2021 03 15.
Article in English | MEDLINE | ID: mdl-33723298

ABSTRACT

Exposure to various antigens derived from house dust mites (HDM) is considered to be a risk factor for development of certain allergic diseases such as atopic asthma, atopic dermatitis, rhinitis and conjunctivitis. Chitin is an insoluble polysaccharide (ß-(1-4)-poly-N-acetyl-D-glucosamine) and a major component in the outer shell of HDMs. Mice exposed to chitin develop asthma-like airway eosinophilia. On the other hand, several lines of evidence show that the effects of chitin on immune responses are highly dependent on the size of chitin particles. In the present study, we show that chitin induced production of IL-33 and TSLP by alveolar and bronchial epithelial cells, respectively, in mice. IL-25, IL-33 and TSLP were reported to be important for group 2 innate lymphoid cell (ILC2)-, but not Th2 cell-, dependent airway eosinophilia in a certain model using chitin beads. Here, we show that-in our murine models-epithelial cell-derived IL-33 and TSLP, but not IL-25, were crucial for activation of resident lung Th2 cells as well as group 2 innate lymphoid cells (ILC2s) to produce IL-5, resulting in development of chitin-induced airway eosinophilia. Our findings provide further insight into the underlying mechanisms of development of HDM-mediated allergic disorders.


Subject(s)
Asthma/etiology , Asthma/metabolism , Cytokines/metabolism , Eosinophilia/etiology , Eosinophilia/metabolism , Interleukin-17/metabolism , Interleukin-33/metabolism , Animals , Asthma/pathology , Biomarkers , Chitin/adverse effects , Disease Models, Animal , Disease Susceptibility , Eosinophilia/pathology , Immunity, Innate , Inflammation Mediators/metabolism , Lymphocyte Subsets/immunology , Lymphocyte Subsets/metabolism , Mice , Mice, Knockout , Thymic Stromal Lymphopoietin
5.
Sci Rep ; 9(1): 17067, 2019 11 19.
Article in English | MEDLINE | ID: mdl-31745167

ABSTRACT

IL-25, a member of the IL-17 family of cytokines, is known to enhance type 2 immune responses, but suppress type 3 (IL-17A)-mediated immune responses. Mice deficient in IL-1 receptor antagonist (Il1rn-/- mice) have excessive IL-1 signaling, resulting in spontaneous development of IL-1-, TNF- and IL-17A-dependent aortitis. We found that expression of II25 mRNA was increased in the aortae of Il1rn-/- mice, suggesting that IL-25 may suppress development of IL-1-, TNF- and IL-17A-dependent aortitis in Il1rn-/- mice by inhibiting type 3-mediated immune responses. However, we unexpectedly found that Il25-/-Il1rn-/- mice showed attenuated development of aortitis, accompanied by reduced accumulation of inflammatory cells such as dendritic cells, macrophages and neutrophils and reduced mRNA expression of Il17a and Tnfa-but not Il4 or Il13-in local lesions compared with Il1rn-/- mice. Tissue-, but not immune cell-, derived IL-25 was crucial for development of aortitis. IL-25 enhanced IL-1ß and TNF production by IL-25 receptor-expressing dendritic cells and macrophages, respectively, at inflammatory sites of aortae of Il1rn-/- mice, contributing to exacerbation of development of IL-1-, TNF- and IL-17A-dependent aortitis in those mice. Our findings suggest that neutralization of IL-25 may be a potential therapeutic target for aortitis.


Subject(s)
Aortitis/immunology , Autoimmune Diseases/immunology , Interleukin 1 Receptor Antagonist Protein/genetics , Interleukins/immunology , Animals , Aortitis/genetics , Aortitis/pathology , Autoimmune Diseases/genetics , Autoimmune Diseases/pathology , Dendritic Cells/immunology , Interleukin 1 Receptor Antagonist Protein/immunology , Interleukin-17/genetics , Interleukin-1beta/metabolism , Interleukins/genetics , Macrophages/immunology , Mice , Mice, Inbred BALB C , Mice, Knockout , Neutrophils/immunology , RNA, Messenger/genetics , Tumor Necrosis Factor-alpha/genetics , Tumor Necrosis Factor-alpha/metabolism
6.
Sci Rep ; 9(1): 15174, 2019 10 23.
Article in English | MEDLINE | ID: mdl-31645588

ABSTRACT

Because cardiomyocyte generation is limited, the turnover of cardiomyocytes in adult heart tissues is much debated. We report here that cardiac pacemaker cells can generate cardiomyocytes from fibroblasts in vitro. Sinoatrial node cells (SANCs) were isolated from adult guinea pig hearts and were cultured at relatively low cell densities. Within a week, a number of fibroblast-like cells were observed to gather around SANCs, and these formed spontaneously beating clusters with cardiomyocyte structures. The clusters expressed genes and proteins that are characteristic of atrial cardiomyocytes. Pharmacological blocking of pacemaker currents inhibited generation of action potentials, and the spontaneous beating were ceased by physically destroying a few central cells. Inhibition of beating during culture also hampered the cluster formation. Moreover, purified guinea pig cardiac fibroblasts (GCFs) expressed cardiac-specific proteins in co-culture with SANCs or in SANC-preconditioned culture medium under electrical stimulation. These results indicate that SANCs can generate cardiomyocytes from cardiac fibroblasts through the influence of humoral factor(s) and electrophysiological activities followed by intracellular Ca2+ oscillations. This potential of SANCs to generate cardiomyocytes indicates a novel mechanism by which cardiomyocytes turns over in the vicinity of pacemaker cells and could be exploited in the development of strategies for cardiac regenerative therapy in adult hearts.


Subject(s)
Biological Clocks , Fibroblasts/cytology , Myocardium/cytology , Myocytes, Cardiac/cytology , Animals , Calcium/metabolism , Cell Aggregation , Cell Differentiation , Cells, Cultured , Electrophysiological Phenomena , Guinea Pigs , Male , Myocytes, Cardiac/metabolism , Phenotype , Sinoatrial Node/cytology , Time Factors , Troponin T/metabolism
7.
Sci Rep ; 8(1): 18052, 2018 12 21.
Article in English | MEDLINE | ID: mdl-30575775

ABSTRACT

Certain proteases derived from house dust mites and plants are considered to trigger initiation of allergic airway inflammation by disrupting tight junctions between epithelial cells. It is known that inhalation of proteases such as house dust mite-derived Der p1 and/or papaya-derived papain caused airway eosinophilia in naïve mice and even in Rag-deficient mice that lack acquired immune cells such as T, B and NKT cells. In contrast, little is known regarding the possible involvement of proteases derived from Aspergillus species (fungal-associated proteases; FAP), which are ubiquitous saprophytic fungi in the environment, in the development of allergic airway eosinophilia. Here, we found that inhalation of FAP by naïve mice led to airway eosinophilia that was dependent on protease-activated receptor-2 (PAR2), but not TLR2 and TLR4. Those findings suggest that the protease activity of FAP, but not endotoxins in FAP, are important in the setting. In addition, development of that eosinophilia was mediated by innate immune cells (ILCs) such as innate lymphoid cells, but not by acquired immune cells such as T, B and NKT cells. Whereas IL-33, IL-25 and thymic stromal lymphopoietin (TSLP) are involved in induction of FAP-induced ILC-mediated airway eosinophilia, IL-33-rather than IL-25 and/or TSLP-was critical for the eosinophilia in our model. Our findings improve our understanding of the molecular mechanisms involved in induction of airway inflammation by FAP.


Subject(s)
Aspergillus/immunology , Cytokines/physiology , Immunity, Innate/physiology , Interleukin-33/physiology , Interleukins/physiology , Peptide Hydrolases/immunology , Pneumonia/immunology , Allergens/immunology , Animals , Aspergillus/enzymology , Aspergillus/metabolism , Immunity, Cellular/physiology , Lung Diseases, Fungal/complications , Lung Diseases, Fungal/enzymology , Lung Diseases, Fungal/genetics , Lung Diseases, Fungal/immunology , Mice , Mice, Inbred C57BL , Mice, Knockout , Peptide Hydrolases/metabolism , Pneumonia/genetics , Pneumonia/metabolism , Pulmonary Eosinophilia/genetics , Pulmonary Eosinophilia/immunology , Thymic Stromal Lymphopoietin
8.
Sci Rep ; 8(1): 15750, 2018 10 24.
Article in English | MEDLINE | ID: mdl-30356086

ABSTRACT

IL-17C, which is a member of the IL-17 family of cytokines, is preferentially produced by epithelial cells in the lung, skin and colon, suggesting that IL-17C may be involved in not only host defense but also inflammatory diseases in those tissues. In support of that, IL-17C was demonstrated to contribute to development of T cell-dependent imiquimod-induced psoriatic dermatitis and T cell-independent dextran sodium sulfate-induced acute colitis using mice deficient in IL-17C and/or IL-17RE, which is a component of the receptor for IL-17C. However, the roles of IL-17C in other inflammatory diseases remain poorly understood. Therefore, we investigated the contributions of IL-17C to development of certain disease models using Il17c-/- mice, which we newly generated. Those mice showed normal development of T cell-dependent inflammatory diseases such as FITC- and DNFB-induced contact dermatitis/contact hypersensitivity (CHS) and concanavalin A-induced hepatitis, and T cell-independent inflammatory diseases such as bleomycin-induced pulmonary fibrosis, papain-induced airway eosinophilia and LPS-induced airway neutrophilia. On the other hand, those mice were highly resistant to LPS-induced endotoxin shock, indicating that IL-17C is crucial for protection against that immunological reaction. Therefore, IL-17C neutralization may represent a novel therapeutic approach for sepsis, in addition to psoriasis and acute colitis.


Subject(s)
Inflammation/etiology , Interleukin-17/physiology , T-Lymphocytes/physiology , Animals , Colitis/therapy , Inflammation/immunology , Interleukin-17/immunology , Mice , Psoriasis/therapy , Sepsis/therapy
9.
Biochem Biophys Res Commun ; 506(3): 429-436, 2018 11 30.
Article in English | MEDLINE | ID: mdl-30352688

ABSTRACT

Levels of IL36α are known to be increased in specimens from patients with atopic dermatitis and psoriasis. In addition, it has been reported that IL-36α is crucial for development of imiquimod-induced psoriatic dermatitis in mice. On the other hand, the role of IL-36α in induction of allergic contact dermatitis/contact hypersensitivity (ACD/CHS) is poorly understood. We found that IL-36α was produced in keratinocytes of mice during imiquimod-induced psoriatic dermatitis, but it was hardly detectable in the skin of mice during either fluorescein isothiocyanate (FITC)- or 1-fluoro-2, 4-dinitrobenzene (DNFB)-induced CHS. Although IL-36α can enhance activation of dendritic cells (DCs) and T cells, in CHS, IL-36α was not essential for DC migration from the skin to draining LNs, but it was required for induction or activation of hapten-specific T cells such as Th/Tc1 or Th17 cells. However, local inflammation, assessed by measurement of ear skin thickness, was comparable between wild-type and IL-36α-deficient mice during both FITC- and DNFB-induced CHS. These observations indicate that IL-36α is involved in induction and/or activation of hapten-specific T-cell subsets in the sensitization phase of CHS, but not essential for induction of local inflammation in the elicitation phase.


Subject(s)
Dermatitis, Contact/immunology , Haptens/immunology , Inflammation/immunology , Interleukin-1/metabolism , T-Lymphocytes/immunology , Animals , Cell Movement , Dendritic Cells/immunology , Dermatitis/immunology , Dermatitis/pathology , Imiquimod , Inflammation/pathology , Keratinocytes/metabolism , Lymph Nodes/pathology , Mice, Inbred C57BL , Psoriasis/immunology , Psoriasis/pathology , Skin/metabolism
10.
Sci Rep ; 8(1): 6639, 2018 04 27.
Article in English | MEDLINE | ID: mdl-29703903

ABSTRACT

IL-31, which is a member of the IL-6 family of cytokines, is produced mainly by activated CD4+ T cells, in particular activated Th2 cells, suggesting a contribution to development of type-2 immune responses. IL-31 was reported to be increased in specimens from patients with atopic dermatitis, and IL-31-transgenic mice develop atopic dermatitis-like skin inflammation, which is involved in the pathogenesis of atopic dermatitis. However, the role of IL-31 in development of contact dermatitis/contact hypersensitivity (CHS), which is mediated by hapten-specific T cells, including Th2 cells, is not fully understood. Therefore, we investigated this using IL-31-deficient (Il31-/-) mice, which we newly generated. We demonstrated that the mice showed normal migration and maturation of skin dendritic cells and induction of hapten-specific T cells in the sensitization phase of FITC-induced CHS, and normal induction of local inflammation in the elicitation phase of FITC- and DNFB-induced CHS. On the other hand, those mice showed reduced scratching frequency and duration during FITC- and/or DNFB-induced CHS. Our findings suggest that IL-31 is responsible for pruritus, but not induction of local skin inflammation, during CHS induced by FITC and DNFB.


Subject(s)
Dermatitis, Contact/pathology , Inflammation/pathology , Interleukins/metabolism , Pruritus/physiopathology , Animals , Dinitrofluorobenzene/administration & dosage , Disease Models, Animal , Fluorescein-5-isothiocyanate/administration & dosage , Inflammation/chemically induced , Interleukins/deficiency , Langerhans Cells/immunology , Mice, Inbred C57BL , Mice, Knockout , Pruritus/chemically induced , Th2 Cells/immunology
11.
J Allergy Clin Immunol ; 142(5): 1500-1509.e10, 2018 11.
Article in English | MEDLINE | ID: mdl-29522843

ABSTRACT

BACKGROUND: In addition to thymic stromal lymphopoietin and IL-33, IL-25 is known to induce TH2 cytokine production by various cell types, including TH2 cells, TH9 cells, invariant natural killer T cells, and group 2 innate lymphoid cells, involved in TH2-type immune responses. Because both TH2-type and TH17-type cells/cytokines are crucial for contact hypersensitivity (CHS), IL-25 can contribute to this by enhancing TH2-type immune responses. However, the precise role of IL-25 in the pathogenesis of fluorescein isothiocyanate-induced CHS is poorly understood. OBJECTIVE: We investigated the contribution of IL-25 to CHS using Il25-/- mice. METHODS: CHS was evaluated by means of measurement of ear skin thickness in mice after fluorescein isothiocyanate painting. Skin dendritic cell (DC) migration, hapten-specific TH cell differentiation, and detection of IL-1ß-producing cells were determined by using flow cytometry, ELISA, and immunohistochemistry, respectively. RESULTS: In contrast to thymic stromal lymphopoietin, we found that IL-25 was not essential for skin DC migration or hapten-specific TH cell differentiation in the sensitization phase of CHS. Unexpectedly, mast cell- and non-immune cell-derived IL-25 was important for hapten-specific TH17 cell-mediated rather than TH2 cell-mediated inflammation in the elicitation phase of CHS by enhancing TH17-related, but not TH2-related, cytokines in the skin. In particular, IL-1ß produced by dermal DCs in response to IL-25 was crucial for hapten-specific TH17 cell activation, contributing to induction of local inflammation in the elicitation phase of CHS. CONCLUSION: Our results identify a novel IL-25 inflammatory pathway involved in induction of TH17 cell-mediated, but not TH2 cell-mediated, CHS. IL-25 neutralization can be a potential approach for treatment of CHS.


Subject(s)
Cytokines/immunology , Dermatitis, Allergic Contact/immunology , Th17 Cells/immunology , Animals , Cytokines/genetics , DNA-Binding Proteins/genetics , Female , Male , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Nuclear Receptor Subfamily 1, Group F, Member 3/genetics , STAT6 Transcription Factor/genetics , Thymic Stromal Lymphopoietin
12.
Biochem Biophys Rep ; 12: 62-65, 2017 Dec.
Article in English | MEDLINE | ID: mdl-28955793

ABSTRACT

Interleukin (IL)-25, which is a member of the IL-17 family of cytokines, induces production of such Th2 cytokines as IL-4, IL-5, IL-9 and/or IL-13 by various types of cells, including Th2 cells, Th9 cells and group 2 innate lymphoid cells (ILC2). On the other hand, IL-25 can suppress Th1- and Th17-associated immune responses by enhancing Th2-type immune responses. Supporting this, IL-25 is known to suppress development of experimental autoimmune encephalitis, which is an IL-17-mediated autoimmune disease in mice. However, the role of IL-25 in development of IL-17-mediated arthritis is not fully understood. Therefore, we investigated this using IL-1 receptor antagonist-deficient (IL-1Ra-/-) mice, which spontaneously develop IL-17-dependent arthritis. However, development of spontaneous arthritis (incidence rate, disease severity, proliferation of synovial cells, infiltration of PMNs, and bone erosion in joints) and differentiation of Th17 cells in draining lymph nodes in IL-25-/- IL-1Ra-/- mice were similar to in control IL-25+/+ IL-1Ra-/- mice. These observations indicate that IL-25 does not exert any inhibitory and/or pathogenic effect on development of IL-17-mediated spontaneous arthritis in IL-1Ra-/- mice.

13.
Allergol Int ; 65(4): 459-465, 2016 Oct.
Article in English | MEDLINE | ID: mdl-27209052

ABSTRACT

BACKGROUND: T cell immunoglobulin domain and mucin domain-containing molecule 3 (TIM-3), which is preferentially expressed on Th1 cells rather than Th2 cells, is considered to be a negative regulator of Th1 cell function. This suggests that TIM-3 indirectly enhances Th2-type immune responses by suppressing Th1 cell function. METHODS: To investigate TIM-3's possible involvement in Th2-type acute and chronic airway inflammation, wild-type and TIM-3-deficient (TIM-3-/-) mice were sensitized and challenged with a house dust mite (HDM) extract. Airway inflammation and the number of inflammatory cells in bronchoalveolar lavage fluids (BALFs) in the mice were determined by histological analysis and with a hemocytometer, respectively. Expression of mRNA in the lungs was determined by quantitative PCR, while the levels of cytokines in the BALFs and IgE in sera were determined by ELISA. RESULTS: Despite constitutive expression of TIM-3 mRNA in the lungs, the number of eosinophils in bronchoalveolar lavage fluids (BALFs) and the score of pulmonary inflammation were comparable between wild-type and TIM-3-/- mice during both acute and chronic HDM-induced airway inflammation. On the other hand, the number of lymphocytes in the BALFs of TIM-3-/- mice was significantly increased compared with wild-type mice during HDM-induced chronic, but not acute, airway inflammation, while the levels of Th2 cytokines in the BALFs and HDM-specific IgG1 and IgG2a and total IgE in the sera were comparable in both groups. CONCLUSIONS: Our findings indicate that, in mice, TIM-3 is not essential for development of HDM-induced acute or chronic allergic airway inflammation, although it appears to be involved in reduced lymphocyte recruitment during HDM-induced chronic allergic airway inflammation.


Subject(s)
Antigens, Dermatophagoides/immunology , Hepatitis A Virus Cellular Receptor 2/genetics , Inflammation/genetics , Inflammation/immunology , Respiratory Tract Diseases/genetics , Respiratory Tract Diseases/immunology , Animals , Bronchoalveolar Lavage Fluid/immunology , Cytokines , Disease Models, Animal , Immunoglobulin E/immunology , Inflammation/metabolism , Inflammation/pathology , Mice , Mice, Knockout , Respiratory Tract Diseases/pathology , Th1 Cells/immunology , Th1 Cells/metabolism , Th2 Cells/immunology , Th2 Cells/metabolism
14.
Biochem Biophys Rep ; 5: 191-195, 2016 Mar.
Article in English | MEDLINE | ID: mdl-28955823

ABSTRACT

IL-25, IL-33 and TSLP, which are produced predominantly by epithelial cells, can induce production of Th2-type cytokines such as IL-4, IL-5 and/or IL-13 by various types of cells, suggesting their involvement in induction of Th2-type cytokine-associated immune responses. It is known that Th2-type cytokines contribute to host defense against malaria parasite infection in mice. However, the roles of IL-25, IL-33 and TSLP in malaria parasite infection remain unclear. Thus, to elucidate this, we infected wild-type, IL-25-/-, IL-33-/- and TSLP receptor (TSLPR)-/- mice with Plasmodium berghei (P. berghei) ANKA, a murine malaria strain. The expression levels of IL-25, IL-33 and TSLP mRNA were changed in the brain, liver, lung and spleen of wild-type mice after infection, suggesting that these cytokines are involved in host defense against P. berghei ANKA. However, the incidence of parasitemia and survival in the mutant mice were comparable to in the wild-type mice. These findings indicate that IL-25, IL-33 and TSLP are not critical for host defense against P. berghei ANKA.

15.
Biochem Biophys Rep ; 7: 119-123, 2016 Sep.
Article in English | MEDLINE | ID: mdl-28955898

ABSTRACT

TSLP induces Th2 cytokine production by Th2 cells and various other types of cells, thereby contributing to Th2-type immune responses and development of allergic disorders. We found that house dust mite (HDM) extract induced TSLP production by nasal epithelial cells, suggesting that TSLP may be involved in development of HDM-induced allergic rhinitis (AR). To investigate that possibility in greater detail, wild-type and TSLP receptor-deficient (TSLPR-/-) mice on the C57BL/6J background were repeatedly treated intranasally with HDM extract. The frequency of sneezing, numbers of eosinophils and goblet cells, thickness of submucosal layers, serum levels of total IgE and HDM-specific IgG1, and levels of IL-4, IL-5 and IL-13 in the culture supernatants of HDM-stimulated LN cells were comparable in the two mouse strains. Those findings indicate that, in mice, TSLPR is not crucial for development of HDM-induced AR.

16.
Immunity ; 43(1): 175-86, 2015 Jul 21.
Article in English | MEDLINE | ID: mdl-26200013

ABSTRACT

House dust mite-derived proteases contribute to allergic disorders in part by disrupting epithelial barrier function. Interleukin-33 (IL-33), produced by lung cells after exposure to protease allergens, can induce innate-type airway eosinophilia by activating natural helper (NH) cells, a member of group 2 innate lymphoid cells (ILC2), to secrete Th2 type-cytokines. Because IL-33 also can induce mast cells (MCs) to secrete Th2 type-cytokines, MCs are thought to cooperate with NH cells in enhancing protease or IL-33-mediated innate-type airway eosinophilia. However, we found that MC-deficient Kit(W-sh/W-sh) mice exhibited exacerbated protease-induced lung inflammation associated with reduced numbers of regulatory T (Treg) cells. Moreover, IL-2 produced by IL-33-stimulated MCs promoted expansion of numbers of Treg cells, thereby suppressing development of papain- or IL-33-induced airway eosinophilia. We have thus identified a unique anti-inflammatory pathway that can limit induction of innate-type allergic airway inflammation mediated by NH cells.


Subject(s)
Inflammation/immunology , Interleukin-2/immunology , Interleukins/immunology , Mast Cells/immunology , T-Lymphocytes, Regulatory/immunology , Animals , Bronchoalveolar Lavage Fluid/cytology , Bronchoalveolar Lavage Fluid/immunology , Cells, Cultured , Eosinophilia/chemically induced , Humans , Interleukin-10/immunology , Interleukin-2/genetics , Interleukin-33 , Interleukins/genetics , Interleukins/pharmacology , Lung/cytology , Lung/immunology , Lymphocyte Activation/drug effects , Lymphocyte Activation/immunology , Mice , Mice, Inbred C57BL , Mice, Knockout , Papain/pharmacology , Proto-Oncogene Proteins c-kit/genetics , Pyroglyphidae/immunology , Th2 Cells/immunology
17.
Pediatr Radiol ; 45(10): 1472-9, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26115723

ABSTRACT

BACKGROUND: Lower tube voltage has advantages for CT angiography, such as improved contrast OBJECTIVE: To evaluate the image quality of low-voltage (70 kV) CT for congenital heart disease and the ability of sinogram-affirmed iterative reconstruction to improve image quality. MATERIALS AND METHODS: Forty-six children with congenital heart disease (median age: 109 days) were examined using dual-source CT. Scans were performed at 80 kV and 70 kV in 21 and 25 children, respectively. A nonionic iodinated contrast medium (300 mg I/ml) was used for the 80-kV protocol. The contrast medium was diluted to 75% (225 mgI/mL) with saline for the 70-kV protocol. Image noise was measured in the two protocols for each group by extracting the standard deviations of a region of interest placed on the descending aorta. We then determined whether sinogram-affirmed iterative reconstruction reduced the image noise at 70 kV. RESULTS: There was more noise at 70 kV than at 80 kV (29 ± 12 vs 20 ± 4.8; P < 0.01). Sinogram-affirmed iterative reconstruction with grade 4 strength settings improved the noise (20 ± 5.9; P < 0.01) for the 70-kV group. CONCLUSION: Sinogram-affirmed iterative reconstruction improved the image quality of CT in congenital heart disease.


Subject(s)
Coronary Angiography/methods , Heart Defects, Congenital/diagnostic imaging , Radiographic Image Interpretation, Computer-Assisted/methods , Tomography, X-Ray Computed/methods , Contrast Media , Female , Humans , Infant , Infant, Newborn , Male , Radiation Dosage , Radiographic Image Enhancement , Reproducibility of Results , Retrospective Studies
18.
PLoS One ; 10(4): e0123584, 2015.
Article in English | MEDLINE | ID: mdl-25901797

ABSTRACT

BACKGROUND: Viral infection is one of the risk factors for asthma exacerbation. However, which pathogens are related to asthma exacerbation in adults remains unclear. OBJECTIVE: The relation between various infections and adult asthma exacerbations was investigated in clinical practice. METHODS: The study subjects included 50 adult inpatients due to asthma exacerbations and 20 stable outpatients for comparison. The pathogens from a nasopharyngeal swab were measured by multiplex PCR analysis. RESULTS: Asthma exacerbations occurred after a common cold in 48 inpatients. The numbers of patients with viral, bacterial, or both infections were 16, 9, and 9, respectively. The dominant viruses were rhinoviruses, respiratory syncytial virus, influenza virus, and metapneumovirus. The major bacteria were S. pneumoniae and H. influenzae. Compared to pathogen-free patients, the patients with pathogens were older and non-atopic and had later onset of disease, lower FeNO levels, lower IgE titers, and a higher incidence of comorbid sinusitis, COPD, or pneumonia. Compared to stable outpatients, asthma exacerbation inpatients had a higher incidence of smoking and comorbid sinusitis, COPD, or pneumonia. Viruses were detected in 50% of stable outpatients, but a higher incidence of rhinovirus, respiratory syncytial virus, and metapneumovirus infections was observed in asthma exacerbation inpatients. H. influenzae was observed in stable asthmatic patients. Other bacteria, especially S. pneumoniae, were important in asthma exacerbation inpatients. CONCLUSION: Viral or bacterial infections were observed in 70% of inpatients with an asthma exacerbation in clinical practice. Infection with S. pneumoniae was related to adult asthma exacerbation.


Subject(s)
Asthma/microbiology , Asthma/virology , Bacterial Infections/complications , Virus Diseases/complications , Adult , Aged , Aged, 80 and over , Asthma/complications , Asthma/epidemiology , Comorbidity , Female , Humans , Male , Middle Aged , Nasopharynx/microbiology , Nasopharynx/virology , Pneumonia/epidemiology , Pulmonary Disease, Chronic Obstructive/epidemiology
19.
Biochem Biophys Res Commun ; 453(1): 1-6, 2014 Oct 10.
Article in English | MEDLINE | ID: mdl-25204502

ABSTRACT

IL-17RA is a shared receptor subunit for several cytokines of the IL-17 family, including IL-17A, IL-17C, IL-17E (also called IL-25) and IL-17F. It has been shown that mice deficient in IL-17RA are more susceptible to sepsis than wild-type mice, suggesting that IL-17RA is important for host defense against sepsis. However, it is unclear which ligands for IL-17RA, such as IL-17A, IL-17C, IL-17E/IL-25 and/or IL-17F, are involved in the pathogenesis of sepsis. Therefore, we examined IL-17A, IL-17E/IL-25 and IL-17F for possible involvement in LPS-induced endotoxin shock. IL-17A-deficient mice, but not IL-25- or IL-17F-deficient mice, were resistant to LPS-induced endotoxin shock, as compared with wild-type mice. Nevertheless, studies using IL-6-deficient, IL-21Rα-deficient and Rag-2-deficient mice, revealed that neither IL-6 and IL-21, both of which are important for Th17 cell differentiation, nor Th17 cells were essential for the development of LPS-induced endotoxin shock, suggesting that IL-17A-producing cells other than Th17 cells were important in the setting. In this connection, IL-17A was produced by macrophages, DCs and eosinophils after LPS injection. Taken together, these findings indicate that IL-17A, but not IL-17F or IL-25, is crucial for LPS-induced endotoxin shock. In addition, macrophages, DCs and eosinophils, but not Th17 cells or γδ T cells, may be sources of IL-17A during LPS-induced endotoxin shock.


Subject(s)
Eosinophils/immunology , Interleukin-17/biosynthesis , Myeloid Cells/immunology , Shock, Septic/immunology , Animals , Female , Interleukin-17/deficiency , Interleukin-17/genetics , Interleukin-21 Receptor alpha Subunit/biosynthesis , Interleukin-21 Receptor alpha Subunit/deficiency , Interleukin-21 Receptor alpha Subunit/genetics , Interleukin-6/biosynthesis , Interleukin-6/deficiency , Interleukin-6/genetics , Interleukins/biosynthesis , Interleukins/deficiency , Interleukins/genetics , Lipopolysaccharides/toxicity , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Receptors, Interleukin-17/metabolism , Shock, Septic/etiology , Th17 Cells/immunology
20.
Obes Res Clin Pract ; 8(3): e201-98, 2014.
Article in English | MEDLINE | ID: mdl-24847666

ABSTRACT

Epidemiological studies suggest an association between breakfast skipping and body weight gain, insulin resistance or type 2 diabetes. Time when meal is consumed affects postprandial increase in energy expenditure and blood glucose, and breakfast skipping may reduce 24 h energy expenditure and elevate blood glucose level. The present study evaluated the effect of breakfast skipping on diurnal variation of energy metabolism and blood glucose. The skipped breakfast was compensated by following big meals at lunch and supper. In a randomized repeated-measure design with or without breakfast, eight males stayed twice in a room-size respiratory chamber. Blood glucose was recorded with a continuous glucose monitoring system. Breakfast skipping did not affect 24 h energy expenditure, fat oxidation and thermic effect of food, but increased overall 24 h average of blood glucose (83 ± 3 vs 89 ± 2 mg/dl, P < 0.05). Unlike 24 h glucose level, 24 h energy expenditure was robust when challenged by breakfast skipping. These observations suggest that changes in glucose homeostasis precede that of energy balance, in the potential sequence caused by breakfast skipping, if this dietary habit has any effect on energy balance.:


Subject(s)
Blood Glucose/metabolism , Breakfast , Diabetes Mellitus, Type 2/blood , Fasting , Insulin Resistance , Weight Gain , Adult , Calorimetry , Cross-Over Studies , Diabetes Mellitus, Type 2/prevention & control , Energy Intake , Energy Metabolism , Fasting/blood , Glycemic Index , Humans , Insulin/blood , Male , Postprandial Period , Time Factors
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