ABSTRACT
BACKGROUND: A new supercooling system using a variable magnetic field has been recently developed. Subzero nonfreezing preservation has been believed to be a beneficial method because of the lower metabolic rate. The purpose of this study was to evaluate the hemodynamic and metabolic effects of rat heart preservation in a variable magnetic field without cryoprotectants. METHODS: Rat hearts were perfused ex vivo for 120 min after 24-hr preservation in two groups (n=6 each): (1) conventional storage group, in which the hearts were stored at 4°C, and (2) the subzero group, in which the hearts were preserved at -3°C in a variable magnetic field. RESULTS: Reperfusion cardiac performance after preservation was significantly preserved in the subzero group compared with the conventional group with respect to heart rate, coronary flow, the peak positive dP/dt, and the peak negative dP/dt (P<0.05). Edema after reperfusion was significantly decreased (P<0.05), and the adenosine triphosphate level was higher in the subzero group (P<0.05). CONCLUSIONS: The rat hearts preserved in a variable magnetic field at -3°C showed better hemodynamic and metabolic performance than those preserved using conventional storage at 4°C.
Subject(s)
Cold Temperature , Cryopreservation , Heart Transplantation , Magnetic Fields , Myocardial Reperfusion Injury/prevention & control , Organ Preservation/methods , Adenosine Triphosphate/metabolism , Animals , Cold Temperature/adverse effects , Coronary Circulation , Heart Rate , Magnetic Fields/adverse effects , Myocardial Contraction , Myocardial Reperfusion Injury/etiology , Myocardial Reperfusion Injury/metabolism , Myocardial Reperfusion Injury/physiopathology , Myocardium/metabolism , Organ Preservation/adverse effects , Rats , Rats, Wistar , Time Factors , Ventricular Function, Left , Ventricular Pressure , Water/metabolismABSTRACT
The case involved a 26-year-old woman with Marfan syndrome (MFS) and severe mitral valve regurgitation who hoped to bear a child. Anticipating future surgery to treat cardiovascular disease via a median sternotomy, we performed mitral annuloplasty via a right anterior thoracotomy. Mitral valve repair for mitral valve regurgitation via a right anterior thoracotomy is one of the most beneficial procedures for patients with MFS.
