ABSTRACT
Two novel antitumour bicyclic hexapeptides, named RA-XV and -XVI, were isolated from Rubia cordifolia and their structures were characterized by spectroscopic and chemical means.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Peptides, Cyclic/pharmacology , Plant Proteins/pharmacology , Amino Acid Sequence , Animals , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Drug Screening Assays, Antitumor , Leukemia P388/drug therapy , Magnetic Resonance Spectroscopy , Molecular Sequence Data , Molecular Structure , Oligopeptides/chemistry , Oligopeptides/isolation & purification , Oligopeptides/pharmacology , Peptides, Cyclic/chemistry , Peptides, Cyclic/isolation & purification , Plant Proteins/chemistry , Plant Proteins/isolation & purification , Tumor Cells, CulturedABSTRACT
1H NMR spectroscopy has been used to demonstrate specific binding of rat 80S ribosomes to the major conformer of an antitumor bicyclic hexapeptidic glucoside, RA-XII, isolated from Rubia cordifolia, in a fast exchange process.
Subject(s)
Antineoplastic Agents, Phytogenic/metabolism , Peptides, Cyclic/metabolism , Ribosomes/metabolism , Animals , In Vitro Techniques , Magnetic Resonance Spectroscopy , Molecular Conformation , RatsABSTRACT
Four new bicyclic hexapeptides, named as RA-XI, -XII, XIII and -XIV were isolated from Rubia cordifolia and showed potent antitumor activities against P-388. The structures were elucidated from spectroscopic and chemical evidences. RA-XII, XIII and -XIV were proved to be unique bicyclic hexapeptidic glucosides.
Subject(s)
Antineoplastic Agents/isolation & purification , Oligopeptides/isolation & purification , Peptides, Cyclic/isolation & purification , Plants, Medicinal/chemistry , Amino Acid Sequence , Animals , Antineoplastic Agents/pharmacology , Leukemia P388/drug therapy , Leukemia P388/pathology , Mice , Molecular Sequence Data , Oligopeptides/pharmacology , Peptides, Cyclic/pharmacology , Tumor Cells, CulturedABSTRACT
The separation of cold drugs and neuroleptics by high-performance liquid chromatogarphy with the porous polymer resin DVB-MCL-O (or 11-30-0), which is a styrene-divinylbenzene-methyl methacrylate copolymer substituted with hydroxymethyl groups, was studied. This copolymer was compared with the commercial porous polymers Hitachi gel 3011, 3011-0 and 3030. A very small theoretical plate height was obtained by using DVB-MCL-O and methanol-ammonia solution (99:1) as the stationary and mobile phases, respectively. This combination was found to be the most suitable for the rapid separation of condensed aromatic ring compounds.
