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Plant Cell Physiol ; 43(1): 136-40, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11828032

ABSTRACT

The sequence requirement of the ACGT-containing abscisic acid response element (ABRE) was analyzed by systematically substituting the bases surrounding the ACGT-core of motif A, the principal ABRE of the rice gene, OSEM: This was done within the context of a 55-bp promoter fragment that minimally confers ABA-responsiveness to a heterologous promoter. Based on this analysis, the sequence requirement of the ACGT-containing ABRE was determined as ACGTG G/T C, which matched very well with the consensus derived from sequence comparison of ABA-responsive promoters.


Subject(s)
Abscisic Acid/pharmacology , Plant Growth Regulators/pharmacology , Response Elements/genetics , Base Sequence , Binding Sites/genetics , Cloning, Molecular , DNA, Plant/genetics , DNA-Binding Proteins/genetics , G-Box Binding Factors , Gene Expression Regulation/drug effects , Glucuronidase/drug effects , Glucuronidase/genetics , Glucuronidase/metabolism , Mutation , Oryza/drug effects , Oryza/genetics , Plant Proteins/genetics , Promoter Regions, Genetic/genetics , Recombinant Fusion Proteins/drug effects , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Transcription Factors/genetics
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