1.
Biosci Biotechnol Biochem
; 87(10): 1249-1253, 2023 Sep 21.
Article
in English
| MEDLINE
| ID: mdl-37475702
ABSTRACT
YcjT is a kojibiose phosphorylase found in Escherichia coli. We found that sucrose was a good acceptor of YcjT in reverse phosphorolysis using ß-d-glucose 1-phosphate as a donor. The product was identified as ß-d-fructofuranosyl α-d-glucopyranosyl-(1â2)-α-d-glucopyranoside. This sugar was also synthesized from sucrose and maltose using YcjT and maltose phosphorylase and promoted the growth of the probiotic Bifidobacterium breve.