1.
Protein Pept Lett
; 12(2): 207-10, 2005 Feb.
Article
in English
| MEDLINE
| ID: mdl-15723648
ABSTRACT
We developed a simple immobilisation method for His-tagged enzymes on a microchannel surface. It facilitates immobilisation of protein molecule on microchannel surface through Ni-complex, using crude or purified protein solutions. By this method, we could immobilize proteins on microcapillary constantly. This method might be useful for further development of microreactor with reversibly immobilized enzymes.