ABSTRACT
MicroRNAs (miRNAs) fine-tune cellular signaling by regulating expression of signaling proteins, and aberrant expression of miRNAs is observed in many cancers. The tyrosine kinase c-Src is upregulated in various human cancers, but the molecular mechanisms underlying c-Src-mediated tumor progression remain unclear. In previous investigations of miRNA-mediated control of c-Src-related oncogenic pathways, we identified miRNAs that were downregulated in association with c-Src transformation and uncovered the signaling networks by predicting their target genes, which might act cooperatively to control tumor progression. Here, to further elucidate the process of cell transformation driven by c-Src, we analyzed the expression profiles of miRNAs in a doxycycline-inducible Src expression system. We found that miRNA (miR)-129-1-3p was downregulated in the early phase of c-Src-induced cell transformation, and that reexpression of miR-129-1-3p disrupted c-Src-induced cell transformation. In addition, miR-129-1-3p downregulation was tightly associated with tumor progression in human colon cancer cells/tissues. Expression of miR-129-1-3p in human colon cancer cells caused morphological changes and suppressed tumor growth, cell adhesion, and invasion. We also identified c-Src and its critical substrate Fer, and c-Yes, a member of the Src family of kinases, as novel targets of miR-129-1-3p. Furthermore, we found that miR-129-1-3p-mediated regulation of c-Src/Fer and c-Yes is important for controlling cell adhesion and invasion. Downregulation of miR-129-1-3p by early activation of c-Src increases expression of these target genes and synergistically promotes c-Src-related oncogenic signaling. Thus, c-Src-miR-129-1-3p circuits serve as critical triggers for tumor progression in many human cancers that harbor upregulation of c-Src.
Subject(s)
CSK Tyrosine-Protein Kinase/metabolism , Cell Transformation, Neoplastic/metabolism , Colonic Neoplasms/metabolism , Down-Regulation , MicroRNAs/genetics , Proto-Oncogene Proteins pp60(c-src)/metabolism , Animals , Cell Movement , Cell Proliferation , Cell Transformation, Neoplastic/genetics , Colonic Neoplasms/genetics , Disease Progression , Gene Expression Regulation, Neoplastic , HCT116 Cells , HT29 Cells , Humans , Mice , Neoplasm TransplantationABSTRACT
BACKGROUND: The autoantigen for the major type of linear IgA bullous dermatosis (LAD, lamina lucida type) is the shed ectodomain of BP180. However, it is unknown why most LAD sera react with the shed ectodomain but not with the intact BP180/type XVII collagen. OBJECTIVE: The aim of this study was to characterize the unique cleavage-dependent epitope(s) in the shed ectodomain. METHODS: We used a monoclonal antibody (MAb-1337) and six LAD sera, which reacted preferentially with the shed ectodomain of BP180. The location and characteristics of the epitopes for these antibodies were analyzed mainly by immunoblotting using chimeric bovine-human BP180 mammalian recombinant proteins and variously truncated bacterial recombinant proteins. RESULTS: LAD sera and MAb-1337 reacted with the plasmin-digested products of full-length BP180. Four of the six LAD sera reacted to a bacterial recombinant protein consisting of the human non-collagenous 16th A (NC16A) and the collagenous 15th (C15) domains, while these sera were negative or only faintly reactive with the NC16A and C15 recombinants. The epitope for MAb-1337 was localized to the COOH-terminal 21 amino acid region within the NC16A domain. CONCLUSION: The results in this study indicate that the major epitopes for LAD sera are formed or exposed by a cleavage-induced conformational change, but not by a post-translational modification that occurs only in the shed ectodomain, and are located at the boundary between the NC16A and C15 domains.
Subject(s)
Collagen/chemistry , Epitopes/chemistry , Skin Diseases, Vesiculobullous/immunology , Amino Acid Sequence , Animals , Autoantigens/chemistry , Cattle , Cell Line, Tumor , Culture Media, Conditioned/chemistry , Epitope Mapping , Fibrinolysin/chemistry , HeLa Cells , Humans , Immunoglobulin A/chemistry , Mice , Microscopy, Fluorescence , Molecular Sequence Data , Non-Fibrillar Collagens/chemistry , Protein Processing, Post-Translational , Protein Structure, Tertiary , Recombinant Proteins/chemistry , Transfection , Collagen Type XVIIABSTRACT
Hemidesmosomes are cell-to-matrix adhesion complexes anchoring keratinocytes to basement membranes. For the first time, we present a method to prepare a fraction from human cultured cells that are highly enriched in hemidesmosomal proteins. Using DJM-1 cells derived from human squamous cell carcinoma, accumulation of hemidesmosomes was observed when these cells were cultured for more than 10 days in a commercial serum-free medium without supplemental calcium. Electron microscopy demonstrated that numerous electron-dense adhesion structures were present along the basal cell membranes of DJM-1 cells cultured under the aforementioned conditions. After removing cellular materials using an ammonia solution, hemidesmosomal proteins and deposited extracellular matrix were collected and separated by electrophoresis. There were eight major polypeptides, which were determined to be plectin, BP230, BP180, integrin α6 and ß4 subunits, and laminin-332 by immunoblotting and mass spectrometry. Therefore, we designated this preparation as a hemidesmosome-rich fraction. This fraction contained laminin-332 exclusively in its unprocessed form, which may account for the promotion of laminin deposition, and minimal amounts of Lutheran blood group protein, a nonhemidesmosomal transmembrane protein. This hemidesmosome-rich fraction would be useful not only for biological research on hemidesmosomes but also for developing a serum test for patients with blistering skin diseases.
