ABSTRACT
Improvement of oral epithelial adhesion to titanium (Ti) may significantly enhance the efficacy of dental implants. We aimed to investigate whether calcium chloride (CaCl2) hydrothermally treated (HT) Ti could promote sealing of the peri-implant epithelium (PIE) around the implant. Right maxillary first molars were extracted from rats and replaced with either CaCl2-HT implants (Ca-HT group), distilled water-HT implants (DW-HT group), or untreated implants (Cont group). After 4 weeks, the implant-PIE interface of the Ca-HT group exhibited a band of immunoreactive laminin-332, similar to the tooth-junctional epithelium interface, which was absent in the Cont and DW-HT groups at the upper portion. We also investigated the effect of Ca-HT on the attachment of rat oral epithelial cells (OECs). OEC adherence onto Ca-HT Ti plates was stronger with higher expression levels of adhesion proteins compared with Cont and DW-HT groups. These results indicate that HT with CaCl2 improves the integration of soft tissue cells with the Ti implant at 4 weeks after implantation, which might facilitate the development of a soft tissue barrier around the implant.
Subject(s)
Calcium Chloride/pharmacology , Dental Implants , Epithelial Cells/drug effects , Titanium/chemistry , Animals , Blotting, Western , Cell Adhesion/drug effects , Cell Adhesion Molecules/metabolism , Cells, Cultured , Epithelial Cells/metabolism , Immunohistochemistry , Implants, Experimental , Integrin beta4/metabolism , Male , Plectin/metabolism , Rats, Wistar , Surface Properties/drug effects , Time Factors , Wound Healing/drug effects , KalininABSTRACT
To achieve osteoconductivity, Ti-0.5Pt and Ti-6Al-4V-0.5Pt alloys were hydrothermally treated at 200 degrees C in 10 mmol/l CaCl(2) aqueous solution for 24 h (HT-treatment). We conducted histological investigations of the HT-treated materials by using Wistar strain rats (SD rats) to evaluate the usefulness of the treatment. To measure the bone bond strength, the specimens were implanted in the tibia of SD rats, and a pull-out test was conducted. From the early postoperative stages, direct bone contact was obtained for the HT-treated implants. Within 1-4 weeks of implantation, the bone contact ratios and bone bond strengths of the HT-treated implants were higher than those of the non-treated implants. The Ti-0.5Pt and Ti-6Al-4V-0.5Pt alloys with HT-treatment showed the potential to develop a new implant with a high bone bond strength and rapid osteoconduction.
