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1.
Am J Physiol Heart Circ Physiol ; 307(8): H1252-61, 2014 Oct 15.
Article in English | MEDLINE | ID: mdl-25128168

ABSTRACT

Increased aortic stiffness is an early and independent biomarker of cardiovascular disease. Here we tested the hypothesis that vascular smooth muscle cells (VSMCs) contribute significantly to aortic stiffness and investigated the mechanisms involved. The relative contributions of VSMCs, focal adhesions (FAs), and matrix to stiffness in mouse aorta preparations at optimal length and with confirmed VSMC viability were separated by the use of small-molecule inhibitors and activators. Using biomechanical methods designed for minimal perturbation of cellular function, we directly quantified changes with aging in aortic material stiffness. An alpha adrenoceptor agonist, in the presence of N(G)-nitro-l-arginine methyl ester (l-NAME) to remove interference of endothelial nitric oxide, increases stiffness by 90-200% from baseline in both young and old mice. Interestingly, increases are robustly suppressed by the Src kinase inhibitor PP2 in young but not old mice. Phosphotyrosine screening revealed, with aging, a biochemical signature of markedly impaired agonist-induced FA remodeling previously associated with Src signaling. Protein expression measurement confirmed a decrease in Src expression with aging. Thus we report here an additive model for the in vitro biomechanical components of the mouse aortic wall in which 1) VSMCs are a surprisingly large component of aortic stiffness at physiological lengths and 2) regulation of the VSMC component through FA signaling and hence plasticity is impaired with aging, diminishing the aorta's normal shock absorption function in response to stressors.


Subject(s)
Aging , Aorta/physiology , Focal Adhesions/metabolism , Myocytes, Smooth Muscle/physiology , Stress, Mechanical , Vascular Stiffness , Adrenergic Agonists/pharmacology , Animals , Aorta/cytology , Aorta/growth & development , Aorta/metabolism , Enzyme Inhibitors/pharmacology , Hemodynamics , Male , Mice , Mice, Inbred C57BL , Myocytes, Smooth Muscle/drug effects , Myocytes, Smooth Muscle/metabolism , NG-Nitroarginine Methyl Ester/pharmacology , Phenylephrine/pharmacology , Pyrimidines/pharmacology , src-Family Kinases/metabolism
2.
J Physiol ; 590(17): 4145-54, 2012 Sep 01.
Article in English | MEDLINE | ID: mdl-22687615

ABSTRACT

This review focuses on the vascular smooth muscle cells present in the medial layer of the blood vessels wall in the fully differentiated state (dVSMCs). The dVSMC contractile phenotype enables these cells to respond in a highly regulated manner to changes in extracellular stimuli. Through modulation of vascular contractile force and vascular compliance dVSMCs regulate blood pressure and blood flow. The cellular and molecular mechanisms by which vascular smooth muscle contractile functions are regulated are not completely elucidated. Recent studies have documented a critical role for actin polymerization and cytoskeletal dynamics in the regulation of contractile function. Here we will review the current understanding of actin cytoskeletal dynamics and focal adhesion function in dVSMCs in order to better understand actin cytoskeleton connections to the extracellular matrix and the effects of cytoskeletal remodelling on vascular contractility and vascular stiffness in health and disease.


Subject(s)
Actin Cytoskeleton/physiology , Muscle Contraction/physiology , Myocytes, Smooth Muscle/physiology , Actin Cytoskeleton/chemistry , Actins/chemistry , Actins/physiology , Animals , Focal Adhesions/physiology , Humans , Molecular Motor Proteins/chemistry , Molecular Motor Proteins/physiology , Protein Multimerization , Vascular Stiffness/physiology , Vasoconstriction/physiology
3.
Mol Neurodegener ; 4: 33, 2009 Jul 23.
Article in English | MEDLINE | ID: mdl-19627603

ABSTRACT

BACKGROUND: The abnormal accumulation of amyloid-beta peptide is believed to cause malfunctioning of neurons in the Alzheimer's disease brain. Amyloid-beta exists in different assembly forms in the aging mammalian brain including monomers, oligomers, and aggregates, and in senile plaques, fibrils. Recent findings suggest that soluble amyloid-beta oligomers may represent the primary pathological species in Alzheimer's disease and the most toxic form that impairs synaptic and thus neuronal function. We previously reported the isolation of a novel amyloid-beta-degrading enzyme, acyl peptide hydrolase, a serine protease that degrades amyloid-beta, and is different in structure and activity from other amyloid-beta-degrading enzymes. RESULTS: Here we report the further characterization of acyl peptide hydrolase activity using mass spectrometry. Acyl peptide hydrolase cleaves the amyloid-beta peptide at amino acids 13, 14 and 19. In addition, by real-time PCR we found elevated acyl peptide hydrolase expression in brain areas rich in amyloid plaques suggesting that this enzyme's levels are responsive to increases in amyloid-beta levels. Lastly, tissue culture experiments using transfected CHO cells expressing APP751 bearing the V717F mutation indicate that acyl peptide hydrolase preferentially degrades dimeric and trimeric forms of amyloid-beta. CONCLUSION: These data suggest that acyl peptide hydrolase is involved in the degradation of oligomeric amyloid-beta, an activity that, if induced, might present a new tool for therapy aimed at reducing neurodegeneration in the Alzheimer's brain.

4.
J Neurochem ; 100(2): 458-67, 2007 Jan.
Article in English | MEDLINE | ID: mdl-17241160

ABSTRACT

Considerable evidence indicates that the amyloid-beta (Abeta) peptide, a proteolytic fragment of the amyloid precursor protein, is the pathogenic agent in Alzheimer's disease (AD). A number of proteases have been reported as capable of degrading Abeta, among them: neprilysin, insulin-degrading enzyme, endothelin-converting enzyme-1 and -2, angiotensin-converting enzyme and plasmin. These proteases, originating from a variety of cell types, degrade Abeta of various conformational states and in different cellular locations. We report here the isolation of a serine protease from serum-free conditioned medium of human neuroblastoma cells. Tandem mass spectrometry (MS/MS)-based sequencing of the isolated protein identified acyl peptide hydrolase (APH; EC3.4.19.1) as the active peptidase. APH is one of four members of the prolyl oligopeptidase family of serine proteases expressed in a variety of cells and tissues, including erythrocytes, liver and brain, but its precise biological activity is unknown. Here, we describe the identification of APH as an Abeta-degrading enzyme, and we show that the degradation of Abeta by APH isolated from transfected cells is inhibited by APH-specific inhibitors, as well as by synthetic Abeta peptide. In addition, we cloned APH from human brain and from neuroblastoma cells. Most importantly, our results indicate that APH expression in AD brain is lower than in age-matched controls.


Subject(s)
Acyltransferases/metabolism , Amyloid beta-Peptides/metabolism , Culture Media, Conditioned/chemistry , Serine/metabolism , Acyltransferases/genetics , Animals , Autoradiography/methods , Cell Line , Chlorocebus aethiops , Cholinesterase Inhibitors , Culture Media, Conditioned/pharmacology , Dose-Response Relationship, Drug , Enzyme Activation/drug effects , Enzyme Inhibitors/pharmacology , Humans , Isoflurophate/pharmacokinetics , Mutation/physiology , Neuroblastoma/enzymology , Neuroblastoma/pathology , Protein Binding/drug effects , RNA, Messenger/biosynthesis , Reverse Transcriptase Polymerase Chain Reaction/methods , Serine/genetics , Tandem Mass Spectrometry/methods , Transfection , Tritium/pharmacokinetics
5.
J Neurosci Res ; 82(1): 32-42, 2005 Oct 01.
Article in English | MEDLINE | ID: mdl-16118793

ABSTRACT

The amyloid precursor protein (APP) must fulfill important roles based on its sequence conservation from fly to human. Although multiple functions for APP have been proposed, the best-known role for this protein is as the precursor of Abeta peptide, a neurotoxic 39-43-amino acid peptide crucial to the pathogenesis of Alzheimer's disease. To investigate additional roles for APP with an eye toward understanding the molecular basis of the pleiotropic effects ascribed to APP, we isolated proteins that interacted with the plasma membrane isoform of APP. We employed a membrane-impermeable crosslinker to immobilize proteins binding to transmembrane APP in human embryonic kidney (HEK)293 cells expressing APP751 (HEK275) or rat embryonic day 18 primary neurons infected with a virus expressing APP. Notch2 was identified as a potential APP binding partner based on mass spectrometry analysis of APP complexes immunopurified from neurons. To confirm the interaction between Notch2 and APP, we carried out immunoprecipitation studies in HEK275 cells transiently expressing full-length Notch2 using Notch2 antibodies. The results indicated that APP and Notch2 interact in mammalian cells, and confirmed our initial findings. Interestingly, Notch1 also coimmunoprecipitated with APP, suggesting that APP and Notch family members may engage in intermolecular cross talk to modulate cell function. Finally, cotransfection of APP/CFP and Notch2/YFP into COS cells revealed that these two proteins colocalize on the plasma membrane. Intracellularly, however, although some APP and Notch molecules colocalize, others reside in distinct locations. The discovery of proteins that interact with APP may aid in the identification of new functions for APP.


Subject(s)
Amyloid beta-Protein Precursor/metabolism , Receptors, Notch/metabolism , Amyloid beta-Protein Precursor/chemistry , Animals , Blotting, Western/methods , Cell Membrane/physiology , Cells, Cultured , Chlorocebus aethiops , Diagnostic Imaging , Electrophoresis, Polyacrylamide Gel/methods , Embryo, Mammalian , Fluorescent Antibody Technique/methods , Gene Deletion , Humans , Immunoprecipitation/methods , Mass Spectrometry/methods , Mutagenesis , Neurons/physiology , Protein Isoforms/metabolism , Protein Structure, Tertiary/physiology , Rats , Receptor Cross-Talk/physiology , Receptors, Notch/genetics , Subcellular Fractions/metabolism , Time Factors , Transfection
7.
Am J Ther ; 3(1): 9-16, 1996 Jan.
Article in English | MEDLINE | ID: mdl-11856992

ABSTRACT

To compare the modulatory effects of different prostaglandins on collagen gene expression in human chondrocytes, PGE(2), PGE(1), misoprostol (PGE(1) analog), and PGF(2alpha) (10, 50 and 100 ng ml(minus sign1)) were added to human chondrocytes with or without interleukin-1beta (IL-1beta) in the presence of indomethacin to inhibit endogenous prostaglandin synthesis and the effects evaluated on chondrocyte morphology, collagen synthesis, and procollagen mRNA levels. The effects of prostaglandins on the expression of collagen gene regulatory sequences were examined using transient transfection assays of reporter gene constructs in human chondrocytes and BALB/c3T3 fibroblasts, PGE(1), misoprostol, and PGF(2alpha), similar to PGE(2), inhibited type I collagen gene expression in fibroblasts and promoted type II collagen gene expression in chondrocytes. PGE(2), the major inflammatory prostaglandin produced by IL-1-activated chondrocytes and fibroblasts, and PGF(2alpha) were somewhat more potent than the anti-inflammatory prostaglandins PGE(1) and misoprostol in counteracting the IL-1-induced suppression of type II collagen gene expression by chondrocytes and stimulation of type I collagen gene expression by fibroblasts. Rather than promoting degradation of the cartilage matrix in joint diseases, prostaglandins may be somewhat protective, suppressing fibrosis, and maintaining or promoting appropriate cartilage repair.

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