ABSTRACT
We studied the effect of a bioactive peptide complex isolated from bovine serum on the proliferative potential and migration rate of mesenchymal stromal cells in vitro, as well as on the healing of modeled bone defects in rats. This bioregulatory preparation stimulated proliferation of mesenchymal stromal cells from deciduous tooth pulp in vitro, but did not affect the rate of their migration in two-dimensional cultures. In vivo experiments showed that application of this preparation in combination with hydroxyapatite and chitosan gel accelerated bone tissue regeneration, thus ensuring restoration of morphologically normal bone matrix. Thus, cattle blood serum is an available source for the production of bioregulatory preparations for medical purposes.
Subject(s)
Mesenchymal Stem Cells/drug effects , Peptides/pharmacology , Regenerative Medicine/methods , Animals , Cattle , Cell Movement/drug effects , Cell Proliferation/drug effects , Cells, Cultured , Male , Mesenchymal Stem Cells/cytology , Microscopy, Electron, Scanning , Peptides/isolation & purification , Rats , Rats, Wistar , Tissue Engineering/methodsABSTRACT
We studied osteogenesis induction after implantation of porous cryogenically structured 3D scaffolds based on chitosan, alginate, and serum albumin and containing a bioregulator from bovine serum into in vivo modeled experimental bone defect in rats. It was shown that such 3D-matrices are effective as osteoconductors due to their effect on osteoblast precursors. All types of bioregulator-loaded 3D materials promote active bone repair, which manifested in restoration of the dense bone tissue, formation of the bone marrow, and the recovery of osteons on day 14 after surgery. In animals receiving implantation of control 3D scaffolds without the bioregulator, the formation of dense fibrous tissue and spongy bone was observed by this term.
Subject(s)
Osteogenesis/physiology , Tissue Scaffolds/chemistry , Animals , Bone Regeneration/physiology , Male , Mesenchymal Stem Cells/cytology , Osteoblasts/cytology , Porosity , Rats , Rats, Wistar , Tissue EngineeringABSTRACT
We studied the protective effect of bioregulators isolated from the liver and blood serum of mammals under conditions of manifest fibrosis. Fibrosis was induced by CCl4 administration for 30 days and then, the liver was cultured in a roller organotypic culture for 30 days in the presence of bioregulators. Hepatoprotective effect of bioregulators was evaluated on histological sections of the liver at different terms of culturing. Experiments with roller organotypic culture of the liver isolated from animals with in vivo CCl4-induced fibrosis demonstrated the protective effect of bioregulator of the liver origin, while bioregulator isolated from the blood was ineffective.
Subject(s)
Carbon Tetrachloride/toxicity , Liver Cirrhosis/blood , Liver Cirrhosis/metabolism , Liver/drug effects , Liver/metabolism , Animals , Female , Liver Cirrhosis/chemically induced , Male , Mice , Organ Culture Techniques , Rats , Rats, WistarABSTRACT
It has been shown that the membranotropic homeostatic tissue-specific bioregulator isolated from rat liver tissue contains a nanosized peptide-protein complex consisting of low-molecular peptides (16.5 kDa) and a protein from the serum albumin family. This bioregulator modulated the peptide biological activity and determined the tissue specificity.
Subject(s)
Liver/chemistry , Peptides , Serum Albumin , Animals , Peptides/chemistry , Peptides/isolation & purification , Rats , Serum Albumin/chemistry , Serum Albumin/isolation & purificationABSTRACT
Substances of a peptide nature isolated from the hepatopancreas of the king crab Paralithodes camtschaticus exhibited physicochemical properties and membranotropic and specific activities similar to those of membranotropic homeostatic tissue-specific bioregulators previously found in different mammalian and plant tissues. Their biological effect on vertebrate tissues was demonstrated on a model of roller organotypic cultivation of Pleurodeles waltl newt liver tissue.
Subject(s)
Anomura/chemistry , Liver/drug effects , Peptides/chemistry , Animals , Organ Specificity , Peptides/isolation & purification , Peptides/pharmacology , SalamandridaeABSTRACT
Peptide mixtures with molecular weights of 1000-2000 Da and in vivo membrano-trophic activity against mouse hepatocyte culture at very low concentrations were isolated from dill Anethum graveolens L. leaves. It has been found that plant peptides in aqueous solution formed larger nanosized particles of approximately 90 nm with a secondary structure mainly composed of ß-structures and random coil structures. We demonstrated that peptides isolated from A. graveolens in vitro at an ultra-low dosage affected the size of the area of pigmented cells of amphibian liver, which are analogous to Kupffer cells of the mammalian liver, using roller organotypic newt liver culture models.
Subject(s)
Anethum graveolens/chemistry , Peptides , Plant Proteins , Animals , Cells, Cultured , Mice , Peptides/chemistry , Peptides/isolation & purification , Peptides/pharmacology , Plant Proteins/chemistry , Plant Proteins/isolation & purification , Plant Proteins/pharmacology , Protein Structure, Secondary , SalamandridaeABSTRACT
A new bioregulator, a representative of membranotropic homeostatic tissue-specific bioregulators acting in ultralow doses, was isolated from rat testes. A model of roller organotypic culturing of mouse testes was developed and the protective effect of the bioregulator was analyzed.
Subject(s)
Biological Factors/pharmacology , Liver/drug effects , Protective Agents/pharmacology , Testis/chemistry , Testis/drug effects , Animals , Biological Factors/isolation & purification , Cell Survival/drug effects , Chromatography, Reverse-Phase , Histocytochemistry , Liver/ultrastructure , Male , Mice , Protective Agents/isolation & purification , Rats , Testis/ultrastructure , Tissue Culture TechniquesABSTRACT
We studied the effect of combined preparation on the basis of chitosan containing a bioregulator isolated from cattle serum in a concentration of 10(-10) mg/ml on healing of II-IIIa degree skin burns in mammals in vivo.
Subject(s)
Biological Factors/pharmacology , Burns/drug therapy , Chitosan/pharmacology , Salicylic Acid/pharmacology , Skin/injuries , Wound Healing/drug effects , Animals , Biological Factors/blood , Biological Factors/therapeutic use , Cattle , Chitosan/therapeutic use , Gels , Histological Techniques , Male , Rats , Rats, Wistar , Salicylic Acid/therapeutic use , Skin/drug effects , Skin/pathology , Wound Healing/physiologyABSTRACT
We compared wound-healing activity of bioregulators isolated from cattle cornea, serum, and retinal pigment epithelium on in vivo model of experimental corneal injury in rabbits. Bioregulators were instilled into the eye as solutions at a concentration corresponding to 10(-12) mg protein/ml. The animals were sacrificed on day 21 after injury and the corneas were examined histologically. The best wound-healing effect was produced by bioregulators isolated from the cornea and serum and instilled successively into rabbit eyes with an interval of 15-20 min twice a day: multicellular epithelium was observed in the wound, and slight inflammation, in the stroma.
Subject(s)
Cornea/chemistry , Corneal Injuries , Epithelium, Corneal/injuries , Eye Injuries/drug therapy , Retinal Pigment Epithelium/chemistry , Wound Healing/drug effects , Animals , Cattle/blood , Male , RabbitsABSTRACT
We studied the effect of combined preparation on the basis of chitosan containing a bioregulator isolated from cattle serum in a concentration of 10(-10) mg/ml on healing of purulent wounds in mammals in vivo.
Subject(s)
Biological Factors/pharmacology , Chitosan/chemistry , Skin/drug effects , Wound Healing/drug effects , Aggregatibacter actinomycetemcomitans/drug effects , Aggregatibacter actinomycetemcomitans/physiology , Animals , Bacterial Infections/drug therapy , Bacterial Infections/microbiology , Biological Factors/chemistry , Biological Factors/isolation & purification , Cattle , Gels , Male , Mice , Porphyromonas gingivalis/drug effects , Porphyromonas gingivalis/physiology , Skin/injuries , Skin/microbiology , Skin/pathology , Staphylococcus aureus/drug effects , Staphylococcus aureus/physiology , Suppuration/drug therapy , Suppuration/microbiologyABSTRACT
We developed models of in vitro organotypic culturing of newt liver tissue with and without adhesion to the substrate. The effects of bioregulators isolated from mammalian liver, blood serum, and bile were studied on the developed models and their specificity was demonstrated. The state of the liver was evaluated by the area of clusters of pigmented cells and by the number of mitoses in the connective tissue cells of the cortical layer. These bioregulators exhibited their biological effects only under conditions of roller organotypic culturing of newt liver tissue.
Subject(s)
Bile/chemistry , Growth Substances/isolation & purification , Growth Substances/pharmacology , Liver/chemistry , Liver/cytology , Organ Culture Techniques/methods , Animals , Growth Substances/blood , SalamandridaeABSTRACT
Studies of the probability of regulating the regenerative and reparative processes in pathologically modified tissues are reviewed. A ready cell system, providing the realization of reparative and regenerative processes in all organs (cell sources of regeneration), exists in all organisms. The authors suggest that active bioregulators, presented in this paper, are involved in the mechanisms of tissue regeneration by modulating the cell sources of regeneration.
Subject(s)
Cell Differentiation/physiology , Extracellular Matrix Proteins/metabolism , Guided Tissue Regeneration/methods , Multipotent Stem Cells/metabolism , Regeneration/physiology , Signal Transduction/physiology , Extracellular Matrix Proteins/physiology , Humans , Multipotent Stem Cells/physiologyABSTRACT
Experiments on stationary culture of posterior eye and roll-bottle culture of the whole eye from adult water lizards Pleurodeles waltl showed that sclera bioregulator produces a stabilizing effects on adhesion interactions between the sclera, choroid, and pigment epithelium and on the maintenance of viability of sclera fibroblasts and pigment epithelium cells.
Subject(s)
Eye Proteins/administration & dosage , Eye/drug effects , Sclera/physiology , Animals , Choroid , Pigment Epithelium of Eye/drug effects , Pleurodeles , Tissue Culture Techniques/methodsABSTRACT
A new bioregulator operating in ultralow doses corresponding to 10(-17) mg/ml has been isolated from tissue of pigmented epithelium of bovine eyes. It has been established that the functional basis of this bioregulator is a complex of a low molecular weight regulatory peptide (4372 Da) and a modulator consisting of a mixture of proteins with molecular weights of 14.980-66.283 kDa. It has been shown that the regulatory peptide is responsible for membranotropic activity of the bioregulator, and the modulator proteins are responsible for biological action in ultralow doses. The data demonstrate an interrelation between nanocondition of the bioregulator and its ability to show activity in ultralow doses.
Subject(s)
Peptides/physiology , Proteins/physiology , Retinal Pigment Epithelium/chemistry , Animals , Cattle , Chromatography, Affinity , Chromatography, High Pressure Liquid , Particle Size , Peptides/chemistry , Proteins/chemistry , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Structure-Activity RelationshipSubject(s)
Gold Colloid/metabolism , Immunohistochemistry/methods , Lectins/metabolism , Ovum/chemistry , Animals , Blood Group Antigens/metabolism , Female , Glycoproteins/metabolism , Glycosylation , Gold Colloid/chemistry , Lectins/chemistry , Oligosaccharides/metabolism , Perches , Protein BindingABSTRACT
A series of short neurotoxins (molecular weight 3500-5000 D) was isolated from Vietnamese scorpion B. occitanus sp. All these toxins blocked generation of action potentials (this effect depended on their molecular weight), but did not change conduction velocity and excitation threshold of the nerve.
Subject(s)
Myelin Sheath/drug effects , Neurons/drug effects , Neurotoxins/isolation & purification , Neurotoxins/pharmacology , Scorpion Venoms/isolation & purification , Scorpion Venoms/pharmacology , Animals , Chromatography, High Pressure Liquid , Neurotoxins/chemistry , Potassium Channels/metabolism , Scorpion Venoms/chemistry , Scorpions , Sodium Channels/metabolism , Time FactorsABSTRACT
Sialic acids are essential components of cell-surface receptors utilized by influenza viruses. To evaluate the recognition of asialic sugar parts of the receptor, three representative strains of human influenza A and B viruses were tested for their binding of a panel of sialyloligosaccharides. The highest affinity binding carbohydrate determinants recognized by the viruses in a context of different core structures were Neu5Ac alpha 2-3Gal for the type B virus, Neu5Ac alpha 2-6 Gal for the H3 subtype virus, and Neu5Ac alpha 2-6Gal beta 1-4GlcNAc for the H1 subtype virus. Penultimate to these determinants parts of the sialyloligosaccharides studied either contributed less significantly to the binding affinity, or interfered with the binding.
