ABSTRACT
BACKGROUND: Helicobacter pylori (H. pylori) infection is closely associated with gastrointestinal diseases. Our preliminary studies have indicated that H. pylori infection had a significant impact on the mucosal microbiome structure in patients with gastric ulcer (GU) or duodenal ulcer (DU). AIM: To investigate the contributions of H. pylori infection and the mucosal microbiome to the pathogenesis and progression of ulcerative diseases. METHODS: Patients with H. pylori infection and either GU or DU, and healthy individuals without H. pylori infection were included. Gastric or duodenal mucosal samples was obtained and subjected to metagenomic sequencing. The compositions of the microbial communities and their metabolic functions in the mucosal tissues were analyzed. RESULTS: Compared with that in the healthy individuals, the gastric mucosal microbiota in the H. pylori-positive patients with GU was dominated by H. pylori, with significantly reduced biodiversity. The intergroup differential functions, which were enriched in the H. pylori-positive GU patients, were all derived from H. pylori, particularly those concerning transfer RNA queuosine-modification and the synthesis of demethylmenaquinones or menaquinones. A significant enrichment of the uibE gene was detected in the synthesis pathway. There was no significant difference in microbial diversity between the H. pylori-positive DU patients and healthy controls. CONCLUSION: H. pylori infection significantly alters the gastric microbiota structure, diversity, and biological functions, which may be important contributing factors for GU.
Subject(s)
Duodenal Ulcer , Gastric Mucosa , Gastrointestinal Microbiome , Helicobacter Infections , Helicobacter pylori , Stomach Ulcer , Humans , Helicobacter Infections/microbiology , Helicobacter pylori/isolation & purification , Helicobacter pylori/genetics , Duodenal Ulcer/microbiology , Duodenal Ulcer/diagnosis , Male , Female , Middle Aged , Gastric Mucosa/microbiology , Gastric Mucosa/pathology , Stomach Ulcer/microbiology , Adult , Case-Control Studies , Aged , Metagenomics/methods , Duodenum/microbiology , Dysbiosis/microbiologyABSTRACT
BACKGROUND Colorectal cancer (CRC) is a deadly form of cancer worldwide. Heat shock protein 70 (Hsp70) belongs to the family of human HSPs and plays an essential role in multiple cellular developments and in responding to environmental changes. However, studies on the relationship between CRC and the Hsp70 family are rare. MATERIAL AND METHODS Data pertaining to 438 patients with CRC was downloaded from The Cancer Genome Atlas database. To investigate the prognostic significance of the Hsp70 genes, survival and joint-effect analyses were conducted. The correlation between prognosis-related Hsp70 genes and clinical factors in CRC was analyzed using a nomogram. Gene set enrichment analysis (GSEA) was performed to explore the complex enrichment pathway in CRC with the prognosis-related Hsp70 genes. RESULTS According to multivariate Cox regression survival analysis, low expression levels of HSPA1A, HSPA1B, and HSPA1L were correlated with improved overall survival (OS). According to the joint-effects survival analysis, the joint low expression levels of HSPA1A, HSPA1B, and HSPA1L were related to improved OS. The 1-, 3-, 5-, and 10-year survival rates of patients with CRC were predicted by constructing a nomogram model based on HSPA1A, HSPA1B, HSPA1L, and tumor stage. The GSEA results indicated the biological roles of HSPA1A, HSPA1B, and HSPA1L in CRC. CONCLUSIONS Low expression levels of HSPA1A, HSPA1B, and HSPA1L were strongly correlated with improved prognosis in CRC and might serve as latent prognostic biomarkers in CRC.
Subject(s)
Colorectal Neoplasms/genetics , HSP70 Heat-Shock Proteins/genetics , Adult , Aged , Case-Control Studies , Colorectal Neoplasms/metabolism , Databases, Genetic , Female , Genetic Predisposition to Disease/genetics , Genotype , HSP70 Heat-Shock Proteins/metabolism , Humans , Male , Middle Aged , Nomograms , Polymorphism, Single Nucleotide/genetics , Prognosis , Survival AnalysisABSTRACT
Non-alcoholic fatty liver disease (NAFLD) is a common liver disease. Previous studies on the association between Helicobacter pylori (HP) infection and NAFLD are inconsistent. Our study was aimed to find out the relationship between HP infection and NAFLD. We performed a large cross-sectional study in northern Chinese adults in 2015. 13C-urea breath tests were used to determine HP infection status. Abdominal ultrasonography was performed to diagnose NAFLD. Multivariable logistic regression was conducted to identify the association between HP infection and NAFLD. A total of 4081 individuals were included in this study; 2137 (52.36%) participants were HP-positive, and 1022 (47.82%) were diagnosed with NAFLD in HP-positive individuals. The odds ratios (OR) and 95% confidence intervals (CI) of participants with HP infection for NAFLD were 1.20 (1.06-1.36) in crude model and 1.27 (1.07-1.50) in fully adjusted model. When stratified by sex and dyslipidemia, the fully adjusted OR and 95% CI for NAFLD were 1.22 (1.10-1.80) in females and 1.44 (1.18-1.75) in subjects with dyslipidemia. There were not significant increased OR for NAFLD when stratified by age. The study indicate that HP infection is associated with NAFLD, particularly in females and patients with dyslipidemia, suggesting that HP eradication might be an alternative method for the prevention or treatment of NAFLD treatment.
Subject(s)
Helicobacter Infections/epidemiology , Helicobacter pylori/pathogenicity , Liver/diagnostic imaging , Non-alcoholic Fatty Liver Disease/epidemiology , Adult , Breath Tests , China/epidemiology , Cross-Sectional Studies , Female , Helicobacter Infections/diagnostic imaging , Helicobacter Infections/microbiology , Helicobacter Infections/pathology , Helicobacter pylori/isolation & purification , Humans , Liver/microbiology , Liver/pathology , Logistic Models , Male , Middle Aged , Non-alcoholic Fatty Liver Disease/diagnosis , Non-alcoholic Fatty Liver Disease/microbiology , Non-alcoholic Fatty Liver Disease/pathology , Risk Factors , UltrasonographyABSTRACT
OBJECTIVE: To investigate the effect of neutrophil gelatinase-associated lipocalin (NGAL) on prognosis of patients with type 2 hepatorenal syndrome (HRS). METHODS: A total of 54 patients with type 2 HRS were included in the study, and stratified for analysis according to survival status at 6-month followup:survival group, n=25; death group, n=29. Single factor analysis was used to compare the betweengroup differences for levels of plasma NGAL, urine NGAL, renin, aldosterone, and blood biochemical indicators. The Cox proportional hazard regression model was used to assess the prognosis of patients with type 2 HRS. The F-test, t-test, chi-square test, Pearson's correlation analysis, and Cox regression model were used for the statistical analyses. RESULTS: The HRS patients with liver cirrhosis showed significantly lower levels of hemoglobin, platelets and albumin (all P < 0.05), but significantly higher international normalized ratio and levels of aspartate aminotransferase, alanine arninotransferase, total bilirubin, direct bilirubin, serum creatinine, plasma NGAL, urine NGAL, renin and aldosterone (all P < 0.05). Plasma NGAL and urine NGAL were positively correlated with renin, aldosterone, blood creatinine, MELD score, Child-Pugh score and ascites (P < 0.05). The patients in the 6-month survival group showed significantly lower levels of albumin, serum sodium, serum creatinine, plasma NGAL, urine NGAL, renin, and aldosterone than those in the death group (P < 0.05), but significantly higher glomerular filtration rate (vs. death group, P < 0.05). The Cox proportional hazard regression model showed that MELD, plasma NGAL, total bilirubin and creatinine were influencing factors of 6-month prognosis for patients with type 2 HRS (relative risk: 1.214, 1.157, 1.098 and 1.016 respectively). CONCLUSION: Plasma NGAL is high in patients with type 2 FHRS, and is associated with risk of death.
