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1.
Materials (Basel) ; 15(5)2022 Feb 28.
Article in English | MEDLINE | ID: mdl-35269047

ABSTRACT

Zr-6Al-0.1B alloy rich in Zr3Al phase is prepared by hot-pressing sintering. The thermal deformation behavior of sintered Zr-6Al-0.1B is analyzed by isothermal compression tests at deformation temperatures of 950, 1050, and 1150 °C with strain rates of 0.01, 0.1, and 1 s-1. The results indicate that at the early stage of thermal deformation, the stress increases rapidly with the increase of strain and then reaches the peak value. Subsequently, the stress decreases with the increase of strain under the softening effect. On the whole, the true stress-strain curve shifts to the high stress area with the increase of strain rate or the decrease of deformation temperature, so the sintered Zr-6Al-0.1B alloy belongs to the temperature and strain rate sensitive material. For the microstructure evolution of sintered Zr-6Al-0.1B during the isothermal compression, the high strain rate can improve the grain refinement. However, because sintered Zr-6Al-0.1B is a low plastic material, too high strain rate will exceed the deformation capacity of the material, resulting in an increase in defects. The increase of deformation temperature also contributes to grain refinement, but when the temperature is too high, due to the decomposition of Zr3Al phase, the deformation coordination of the material decreases, leading to the increase of the probability of the occurrence of defects. This study verified the feasibility of hot-pressing sintering to prepare Zr-6Al-0.1B alloy rich in Zr3Al phase and laid the foundation of "hot-pressing sintering + canning hot-extrusion" process of Zr-6Al-0.1B alloy components.

2.
Materials (Basel) ; 12(13)2019 Jul 07.
Article in English | MEDLINE | ID: mdl-31284693

ABSTRACT

The high-temperature deformation behavior of Q345 steel is detected by a Gleeble-3800 thermal simulator. The Arrhenius constitutive equation for high-temperature flow stress and the dynamic recrystallization model are constructed. With the secondary development technology, customized modifications are made on existing Deform-3D software. The constructed constitutive model and dynamic recrystallization model are embedded into Deform-3D to realize the secondary development of Deform-3D. The grain size and volume percentage distribution of dynamic recrystallization are obtained by simulating the shear connection process at high temperature and high speed. The results show that the constitutive equation and the dynamic recrystallization model constructed in this paper can be used to predict the evolution of the microstructure. The difference between the prediction results and the experimental data is about 3%. The accuracy of Arrhenius constitutive equation, dynamic recrystallization model and the feasibility of software secondary development are verified.

3.
Biomed Pharmacother ; 101: 852-859, 2018 May.
Article in English | MEDLINE | ID: mdl-29635894

ABSTRACT

PURPOSE: The aim of this study is to investigate the inhibition of cancer growth by pterostilbene through Metastasis-Associated Protein 1 (MTA1) and the histone deacetylase 1 (HDAC1) complex in hepatocellular carcinoma (HCC). METHODS: We investigate the antitumor effects of pterostilbene (PTER) in HCC. The SMMC-7721 hepatoma cell line was cultured and treated with PTER for different time depending on the experiment. After treatment, we tested the cellular expression of proteins by Western blot and the expression of MTA1 mRNA by real-time PCR. And the immunoprecipitation was performed to confirm the acetylation in PTEN. Animal models have been established to confirm the anti-cancer effects of PTER. RESULTS: PTER treatment could downregulate the expression of MTA1, and HDAC1 and elevates the Ac-PTEN ratio in tumors. The results suggest that PTER can decrease the expression of MTA1 and destabilize the MTA1/HDAC1 complex allowing acetylation/activation of PTEN on Lys402 site. The expression of MTA1 may be linked to cell apoptosis and invasion in HCC. CONCLUSION: We demonstrated that PTER suppressed the growth, and invasion of HCC and was effective in regulating the levels of the MTA1/HDAC1/NuRD complex, promoting PTEN acetylation and apoptosis in HCC. Our findings suggest that the novel epigenetic nature of PTER anticancer activity opens up new avenues for primary chemoprevention, as well as anticancer and antimetastatic treatment.


Subject(s)
Carcinoma, Hepatocellular/drug therapy , Carcinoma, Hepatocellular/metabolism , Histone Deacetylase 1/metabolism , Histone Deacetylases/metabolism , Liver Neoplasms/drug therapy , Liver Neoplasms/metabolism , PTEN Phosphohydrolase/metabolism , Repressor Proteins/metabolism , Stilbenes/therapeutic use , Acetylation/drug effects , Animals , Apoptosis/drug effects , Carcinoma, Hepatocellular/genetics , Cell Line, Tumor , Cell Proliferation/drug effects , Chromatin Assembly and Disassembly/drug effects , Down-Regulation/drug effects , Down-Regulation/genetics , Gene Expression Regulation, Neoplastic/drug effects , Gene Knockdown Techniques , Humans , Liver Neoplasms/genetics , Mi-2 Nucleosome Remodeling and Deacetylase Complex/metabolism , Mice, Nude , Models, Biological , Neoplasm Invasiveness , RNA, Messenger/genetics , RNA, Messenger/metabolism , Stilbenes/pharmacology , Trans-Activators , Transcriptional Activation/drug effects , Transcriptional Activation/genetics
4.
Blood ; 107(11): 4484-90, 2006 Jun 01.
Article in English | MEDLINE | ID: mdl-16497971

ABSTRACT

Oncogenic RAS expression occurs in up to 40% of multiple myeloma (MM) cases and correlates with aggressive disease. Since activated RAS induces cyclooxygenase-2 (cox-2) expression in other tumor models, we tested a role for cox-2 in mutant RAS-containing MM cells. We used the ANBL-6 isogenic MM cell lines in which the IL-6-dependent parental line becomes cytokine independent following transfection with mutated N-RAS or K-RAS. Both mutated N-RAS- and K-RAS-expressing ANBL-6 cells demonstrated a selective up-regulation of cox-2 expression and enhanced secretion of PGE2, a product of cox-2. Furthermore, in 3 primary marrow specimens, which contained MM cells expressing mutated RAS, 15% to 40% of tumor cells were positive for cox-2 expression by immunohistochemistry. We used cox-2 inhibitors, NS398 and celecoxib, and neutralizing anti-PGE2 antibody to test whether cox-2/PGE2 was involved in the aggressive phenotype of MM ANBL-6 cells containing mutated RAS. Although these interventions had no effect on IL-6-independent growth or adhesion to marrow stromal cells, they significantly inhibited the enhanced binding of mutant RAS-containing MM cells to fibronectin and the enhanced resistance to melphalan. These results indicate a selective induction of cox-2 in MM cells containing RAS mutations, which results in heightened binding to extracellular matrix protein and chemotherapeutic drug resistance.


Subject(s)
Cell Adhesion/genetics , Cyclooxygenase 2/genetics , Drug Resistance, Neoplasm/genetics , Gene Expression Regulation, Neoplastic , Multiple Myeloma/pathology , Proto-Oncogene Proteins p21(ras)/genetics , Cell Line, Tumor , Cyclooxygenase 2/analysis , Dinoprostone/analysis , Fibronectins/metabolism , Genes, ras/genetics , Humans , Multiple Myeloma/genetics , Mutation , Stromal Cells/cytology
5.
Cancer Res ; 66(4): 2305-13, 2006 Feb 15.
Article in English | MEDLINE | ID: mdl-16489035

ABSTRACT

Mammalian target of rapamycin (mTOR) inhibitors curtail cap-dependent translation. However, they can also induce post-translational modifications of proteins. We assessed both effects to understand the mechanism by which mTOR inhibitors like rapamycin sensitize multiple myeloma cells to dexamethasone-induced apoptosis. Sensitization was achieved in multiple myeloma cells irrespective of their PTEN or p53 status, enhanced by activation of AKT, and associated with stimulation of both intrinsic and extrinsic pathways of apoptosis. The sensitizing effect was not due to post-translational modifications of the RAFTK kinase, Jun kinase, p38 mitogen-activated protein kinase, or BAD. Sensitization was also not associated with a rapamycin-mediated increase in glucocorticoid receptor reporter expression. However, when cap-dependent translation was prevented by transfection with a mutant 4E-BP1 construct, which is resistant to mTOR-induced phosphorylation, cells responded to dexamethasone with enhanced apoptosis, mirroring the effect of coexposure to rapamycin. Thus, sensitization is mediated by inhibition of cap-dependent translation. A high-throughput screening for translational efficiency identified several antiapoptotic proteins whose translation was inhibited by rapamycin. Immunoblot assay confirmed rapamycin-induced down-regulated expressions of XIAP, CIAP1, HSP-27, and BAG-3, which may play a role in the sensitization to apoptosis. Studies in a xenograft model showed synergistic in vivo antimyeloma effects when dexamethasone was combined with the mTOR inhibitor CCI-779. Synergistic effects were associated with an enhanced multiple myeloma cell apoptosis in vivo. This study supports the strategy of combining dexamethasone with mTOR inhibitors in multiple myeloma and identifies a mechanism by which the synergistic effect is achieved.


Subject(s)
Apoptosis/drug effects , Dexamethasone/pharmacology , Protein Kinase Inhibitors/pharmacology , Protein Kinases/metabolism , Adaptor Proteins, Signal Transducing , Animals , Carrier Proteins/metabolism , Cell Cycle Proteins , Cell Line, Tumor , Drug Synergism , Focal Adhesion Kinase 2/metabolism , Humans , MAP Kinase Kinase 4/metabolism , Mice , Mice, Inbred NOD , Mice, SCID , Multiple Myeloma/drug therapy , Multiple Myeloma/enzymology , Multiple Myeloma/pathology , Phosphoproteins/metabolism , Receptors, Glucocorticoid/metabolism , Ribosomal Protein S6 Kinases, 70-kDa/metabolism , Sirolimus/analogs & derivatives , Sirolimus/pharmacology , TOR Serine-Threonine Kinases , bcl-Associated Death Protein/metabolism , p38 Mitogen-Activated Protein Kinases/metabolism
6.
Mol Cancer Ther ; 4(10): 1533-40, 2005 Oct.
Article in English | MEDLINE | ID: mdl-16227402

ABSTRACT

Mammalian target of rapamycin (mTOR) inhibitors, such as rapamycin and CCI-779, have shown preclinical potential as therapy for multiple myeloma. By inhibiting expression of cell cycle proteins, these agents induce G1 arrest. However, by also inhibiting an mTOR-dependent serine phosphorylation of insulin receptor substrate-1 (IRS-1), they may enhance insulin-like growth factor-I (IGF-I) signaling and downstream phosphatidylinositol 3-kinase (PI3K)/AKT activation. This may be a particular problem in multiple myeloma where IGF-I-induced activation of AKT is an important antiapoptotic cascade. We, therefore, studied AKT activation in multiple myeloma cells treated with mTOR inhibitors. Rapamycin enhanced basal AKT activity, AKT phosphorylation, and PI3K activity in multiple myeloma cells and prolonged activation of AKT induced by exogenous IGF-I. CCI-779, used in a xenograft model, also resulted in multiple myeloma cell AKT activation in vivo. Blockade of IGF-I receptor function prevented rapamycin's activation of AKT. Furthermore, rapamycin prevented serine phosphorylation of IRS-1, enhanced IRS-1 association with IGF-I receptors, and prevented IRS-1 degradation. Although similarly blocking IRS-1 degradation, proteasome inhibitors did not activate AKT. Thus, mTOR inhibitors activate PI3-K/AKT in multiple myeloma cells; activation depends on basal IGF-R signaling; and enhanced IRS-1/IGF-I receptor interactions secondary to inhibited IRS-1 serine phosphorylation may play a role in activation of the cascade. In cotreatment experiments, rapamycin inhibited myeloma cell apoptosis induced by PS-341. These results provide a caveat for future use of mTOR inhibitors in myeloma patients if they are to be combined with apoptosis-inducing agents.


Subject(s)
Insulin-Like Growth Factor I/metabolism , Multiple Myeloma/drug therapy , Phosphoproteins/metabolism , Protein Kinase Inhibitors/pharmacology , Protein Kinases/metabolism , Proto-Oncogene Proteins c-akt/metabolism , Receptor, IGF Type 1/metabolism , Animals , Antibiotics, Antineoplastic/pharmacology , Apoptosis/drug effects , Boronic Acids/pharmacology , Bortezomib , Cell Line, Tumor , Enzyme Activation/drug effects , Humans , Insulin Receptor Substrate Proteins , Mice , Mice, SCID , Multiple Myeloma/enzymology , Multiple Myeloma/metabolism , Phosphatidylinositol 3-Kinases/metabolism , Pyrazines/pharmacology , Receptor, IGF Type 1/antagonists & inhibitors , Sirolimus/analogs & derivatives , Sirolimus/pharmacology , TOR Serine-Threonine Kinases , Xenograft Model Antitumor Assays
7.
Blood ; 104(13): 4181-7, 2004 Dec 15.
Article in English | MEDLINE | ID: mdl-15304393

ABSTRACT

In vitro studies indicate the therapeutic potential of mTOR inhibitors in treating multiple myeloma. To provide further support for this potential, we used the rapamycin analog CCI-779 in a myeloma xenograft model. CCI-779, given as 10 intraperitoneal injections, induced significant dose-dependent, antitumor responses against subcutaneous growth of 8226, OPM-2, and U266 cell lines. Effective doses of CCI-779 were associated with modest toxicity, inducing only transient thrombocytopenia and leukopenia. Immunohistochemical studies demonstrated the antitumor responses were associated with inhibited proliferation and angiogenesis, induction of apoptosis, and reduction in tumor cell size. Although CCI-779-mediated inhibition of the p70 mTOR substrate was equal in 8226 and OPM-2 tumor nodules, OPM-2 tumor growth was considerably more sensitive to inhibition of proliferation, angiogenesis, and induction of apoptosis. Furthermore, the OPM-2 tumors from treated mice were more likely to show down-regulated expression of cyclin D1 and c-myc and up-regulated p27 expression. Because earlier work suggested heightened AKT activity in OPM-2 tumors might induce hypersensitivity to mTOR inhibition, we directly tested this by stably transfecting a constitutively active AKT allele into U266 cells. The in vivo growth of the latter cells was remarkably more sensitive to CCI-779 than the growth of control U266 cells.


Subject(s)
Multiple Myeloma/drug therapy , Protein Kinases/metabolism , Sirolimus/analogs & derivatives , Sirolimus/therapeutic use , Animals , Cell Division/drug effects , Humans , Mice , Mice, Inbred NOD , Mice, SCID , Multiple Myeloma/pathology , Sirolimus/toxicity , TOR Serine-Threonine Kinases , Transplantation, Heterologous
8.
Oncogene ; 23(19): 3368-75, 2004 Apr 22.
Article in English | MEDLINE | ID: mdl-15021914

ABSTRACT

The IL-6-induced activation of the phosphatidylinositol-3' kinase (PI3-K)/AKT cascade in multiple myeloma (MM) cells is critical for tumor cell proliferation and viability. Since the IL-6 receptor does not contain binding sites for the p85 regulatory portion of PI3-K, intermediate molecules must play a role. Coimmunoprecipitation studies in MM cell lines demonstrated the IL-6-induced formation of two independent PI3-K-containing complexes: one containing p21 RAS but not STAT-3 and a second containing STAT-3 but not RAS. Both complexes demonstrated IL-6-induced lipid kinase activity. IL-6 also generated kinase activity in a mutant p110 molecule that could not bind p85. Use of dominant-negative (DN) constructs confirmed the presence of two independent pathways of activation: a DN RAS prevented the IL-6-induced generation of lipid kinase activity in the mutant p110 molecule but had no effect on activity generated in the STAT-3-containing complex. In contrast, a DN p85 prevented the generation of kinase activity in the STAT-3-containing complex but had no effect on activity generated in the p110 molecule. Both DN constructs significantly prevented the IL-6-induced activation of AKT. MM cells expressing activating RAS mutations demonstrated enhanced IL-6-independent growth and constitutive PI3-K activity. These data indicate two potential independent pathways of PI3-K/AKT activation in MM cells: one mediated via signaling through RAS which is independent of p85 and a second mediated via p85 and due to a STAT-3-containing complex.


Subject(s)
Interleukin-6/pharmacology , Multiple Myeloma/enzymology , Oncogene Protein p21(ras)/physiology , Phosphatidylinositol 3-Kinases/metabolism , Signal Transduction , Cell Line , DNA-Binding Proteins/physiology , Enzyme Activation , Humans , Phosphatidylinositol 3-Kinases/chemistry , Protein Serine-Threonine Kinases/metabolism , Protein Subunits , Proto-Oncogene Proteins/metabolism , Proto-Oncogene Proteins c-akt , STAT3 Transcription Factor , Trans-Activators/physiology
9.
Dev Biol ; 263(2): 323-9, 2003 Nov 15.
Article in English | MEDLINE | ID: mdl-14597205

ABSTRACT

Lozenge (Lz) is a multifunctional transcription factor that is activated in a pool of pluripotent cells at the beginning of a wave of morphogenesis during Drosophila eye development. Lozenge belongs to the Runx class of transcription factors that includes the mammalian proteins AML1, Runx 2, and Runx 3. These proteins allow a tissue-specific precursor population of cells to attain multiple terminally differentiated fates. We investigated the transcriptional control of lz to determine the mechanism by which precursor populations achieve their identity. We have identified a 251-bp region in the second intron of the lz gene that functions as a minimal eye-specific enhancer. We provide evidence that Sine oculis and Glass are the two major activators of Lz expression during eye development. This work establishes a bridge between early eye specification genes and late cell-specific transcription factors required for terminal determination of cone cells in the eye.


Subject(s)
DNA-Binding Proteins/genetics , Drosophila Proteins/genetics , Drosophila/embryology , Eye/embryology , Transcription Factors/genetics , Transcription, Genetic , Animals , Base Sequence , Drosophila Proteins/physiology , Enhancer Elements, Genetic , Eye Proteins/physiology , Gene Expression Regulation, Developmental , Homeodomain Proteins/physiology , Molecular Sequence Data
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