Promoting the sustainable development of traditional villages: Exploring the comprehensive assessment, spatial and temporal evolution, and internal and external impacts of traditional village human settlements in hunan province.

The protection and development of traditional villages are crucial for improving the human settlement suitability (HSS). The paper takes 703 traditional villages in Hunan Province as the research object and establishes the HSS evaluation system by using the pressure-state-response model. Then this paper introduces the vector autoregressive model to explore the interactions and contributions within the three major subsystems. Finally, this paper adopts Geodetector model and GTWR model to study the external driving effects and temporal-spatial influence mechanisms. The main findings of this paper are as follows. First, the overall trend of the composite index of traditional villages is upward. Its spatial pattern transitions from a low index in the northwest to a medium index in the central region and a high index in the southeast. Second, the state system becomes the main driver of the response system change and it is highly influenced by the pressure system. Distance from medical facilities, Distance from educational institutions, Distance from the intangible cultural heritage sites, and Degree of relief are the four most important driving factors affecting the HSS in Hunan Province. At the same time, Distance to medical facilities and Distance to intangible cultural heritage sites have a positive impact, while Distance to educational institutions and Degree of relief have a negative impact. Fourly, these four factors have a significant spatiotemporal impact on the HSS in the Xiangxi region. This paper provides a scientific basis for the sustainable development and conservation of traditional villages in Hunan from multiple perspectives.

Intramolecular chaperone-assisted dual-anchoring activation (ICDA): a suitable preorganization for electrophilic halocyclization.

The halocyclization reaction represents one of the most common methodologies for the synthesis of heterocyclic molecules. Many efforts have been made to balance the relationship between structure, reactivity and selectivity, including the design of new electrophilic halogenation reagents and the utilization of activating strategies. However, discovering universal reagents or activating strategies for electrophilic halocyclization remains challenging due to the case-by-case practice for different substrates or different cyclization models. Here we report an intramolecular chaperone-assisted dual-anchoring activation (ICDA) model for electrophilic halocyclization, taking advantage of the non-covalent dual-anchoring orientation as the driving force. This protocol allows a practical, catalyst-free and rapid approach to access seven types of small-sized, medium-sized, and large-sized heterocyclic units and to realize polyene-like domino halocyclizations, as exemplified by nearly 90 examples, including a risk-reducing flow protocol for gram-scale synthesis. DFT studies verify the crucial role of ICDA in affording a suitable preorganization for transition state stabilization and X+ transfer acceleration. The utilization of the ICDA model allows a spatiotemporal adjustment to straightforwardly obtain fast, selective and high-yielding synthetic transformations.

Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Ameliorate Aging-Induced BTB Impairment in Porcine Testes by Activating Autophagy and Inhibiting ROS/NLRP3 Inflammasomes via the AMPK/mTOR Signaling Pathway.

As a pivotal player in spermatogenesis, the blood-testis barrier (BTB) made from junction apparatus coexisting in Sertoli cells (SCs) is impaired with an increase in age and ultimately induces spermatogenic dysfunction or even infertility. It has been corroborated that bone marrow mesenchymal stem cell (BMSC) transplantation can efficiently repair and regenerate the testicular function. As vital mediators of cell-to-cell communication, MSC-derived exosomes (Exos) can directly serve as therapeutic agents for tissue repair and regeneration. However, the therapeutic value of BMSC-Exos in aging-induced BTB damage remains to be confirmed. In this study, we explored that the old porcine testes had defective autophagy, which aggravated BTB disruption in SCs. BMSC-Exos could decrease ROS production and NLRP3 inflammasome activation but enhanced autophagy and tight junction (TJ) function in D-gal-triggered aging porcine SCs and mouse model testes, according to in vitro and in vivo experiments. Furthermore, rapamycin, NAC, MCC950, and IL-1Ra restored the TJ function in D-gal-stimulated aging porcine SCs, while BMSC-Exos' stimulatory effect on TJ function was inhibited by chloroquine. Moreover, the treatment with BMSC-Exos enhanced autophagy in D-gal-induced aging porcine SCs by means of the AMPK/mTOR signal transduction pathway. These findings uncovered through the present study that BMSC-Exos can enhance the BTB function in aging testes by improving autophagy via the AMPK/mTOR signaling pathway, thereby suppressing ROS production and NLRP3 inflammasome activation.

Analysis of collaborative control of dairy product supply chain quality based on evolutionary games: Perspectives from government intervention and market failure.

Dairy quality affects the health and quality of life of consumers. Implementing supply chain management and collaborative quality control is an effective way to solve dairy quality problems. Based on the perspective of the combination of market failure and government intervention, this paper analyzes the conditions for the realization of collaborative quality control between dairy farmers and dairy processors. At the same time, this paper uses relevant data to verify the applicability of the model and the accuracy of the conclusions. The findings show that both low yields and high spillovers lead to market failures. When farmers adopt advanced prevention strategies and dairy processors adopt advanced inspection strategies with low yields, basic prevention and basic inspection will be the best combination of quality control strategies for both parties. In this case, the government should subsidize the advanced control strategy to provide adequate subsidies. This will provide incentives for both parties to work together to ensure the quality of dairy products. Secondly, when farmers adopt advanced prevention strategies or dairy processors adopt advanced inspection strategies yields increase but spillover rates are higher. Advanced prevention, basic inspection or basic prevention, advanced inspection would be the optimal combination of strategies for both parties. At this point, the government should increase the penalties. The simulation results further indicate that the government subsidy is more effective for dairy farmers. Government penalties have both the positive and negative reinforcing effects.

Themes and ideologies in China's diplomatic discourse - a corpus-assisted discourse analysis in China's official speeches.

Diplomatic discourse is a formalized form of political communication that significantly influences a country's international perception. However, there is a research gap in the analysis of China's diplomatic discourse, particularly in relation to the speeches available on the official Chinese Foreign Ministry website. This study aims to address this gap by conducting a quantitative and qualitative analysis of China's diplomatic speeches. This study utilizes a quantitative corpus-assisted discourse analysis to explore the prevalent themes in China's official speeches. Additionally, qualitative discourse analysis is employed to examine the ideologies manifested in specific examples from the official speeches. The research combines a corpus-based approach with critical discourse analysis to investigate language use, discourse practices, and social practices. The analysis of China's diplomatic discourse reveals several key themes related to President Xi Jinping's leadership, international relations, and future community and economy. The findings provide valuable insights into China's diplomatic strategies and its international image, emphasizing its commitment to cooperation, development, and peace. This research contributes to a better understanding of China's diplomatic discourse and its role in shaping international perceptions of the country. By highlighting the prevalent themes and ideologies in China's official speeches, the study emphasizes China's commitment to fostering positive international relations. The findings offer valuable insights into China's diplomatic strategies and its efforts to shape its international image.

Effects of sediment dredging on freshwater system: a comprehensive review.

As a common geo-engineering method to control internal load of nutrients and pollutants, sediment dredging has been used in many freshwater basins and has achieved certain effects. However, dredging can disturb water bodies and substrates and cause secondary pollution. It negatively affects the water environment system mainly from the following aspects. Dredging suddenly changes the hydrological conditions and many physical indicators of the water body, which will cause variations in water physicochemical properties. For example, changes in pH, dissolved oxygen, redox potential, transparency, and temperature can lead to a series of aquatic biological responses. On the other hand, sediment resuspension and deep-layer sediment exposure can affect the cycling of nutrients (e.g., nitrogen, phosphorus), the release and valence conversion of heavy metals, and the desorption and degradation of organic pollutants in the overlying water. This can further affect the community structure of aquatic organisms. The aim of this paper is to analyze the relevant literature on freshwater sediment dredging, and to summarize the current knowledge of the potential environmental risks caused by the dredging and utilization of freshwater sediments. Based on this, the paper attempts to propose suggestions to mitigate these adverse environmental impacts. These are significant contributions to the development of environmentally friendly freshwater sediment dredging technologies.

Comprehensive evaluation of community human settlement resilience and spatial characteristics based on the supply-demand mismatch between health activities and environment: a case study of downtown Shanghai, China.

INTRODUCTION: Under globalization, human settlement has become a major risk factor affecting life. The relationship between humans and the environment is crucial for improving community resilience and coping with globalization. This study focuses on the key contradictions of community development under globalization, exploring community resilience by analyzing the mismatch between residents' health activities and the environment. METHODS: Using data from Shanghai downtown, including land use, Sports app, geospatial and urban statistics, this paper constructs a comprehensive community resilience index (CRI) model based on the DPSIR model. This model enables quantitative analysis of the spatial and temporal distribution of Community Human Settlement Resilience (CR). Additionally, the paper uses geodetector and Origin software to analyze the coupling relationship between drivers and human settlement resilience. RESULTS: i) The scores of CR showed a "slide-shaped" fluctuation difference situation; ii) The spatial pattern of CR showed a "pole-core agglomeration and radiation" type and a "ring-like agglomeration and radiation" type. iii) Distance to bus stops, average annual temperature, CO2 emissions, building density and number of jogging trajectories are the dominant factors affecting the resilience level of community human settlement. CONCLUSION: This paper contributes to the compilation of human settlement evaluation systems globally, offering insights into healthy community and city assessments worldwide. The findings can guide the creation of similar evaluation systems and provide valuable references for building healthy communities worldwide.

Quantitative assessment, spatial and temporal characteristics, and dynamic evolution of carbon emissions from animal husbandry in China: 2001-2020.

Carbon emissions from animal agriculture are a major source of global greenhouse gases. This paper measures the spatial and temporal characteristics and evolution patterns of carbon emissions from livestock farming in China and 31 provinces from 2001 to 2020 based on IPCC coefficients. The paper also uses Moran's I index, kernel density estimation, and spatial Markov chains for the analysis. The results show that the total carbon emissions from China's livestock sector show a fluctuating downward trend. And livestock carbon emissions are concentrated in areas with better resource endowments, with grassland and grain-producing areas dominating China's livestock carbon emissions. The spatial analysis shows that the spatial correlation of the national livestock carbon emissions is increasing, showing prominent local aggregation characteristics, mainly in the form of high-high and low-low aggregation. The transfer of carbon emissions from China's livestock industry shows strong spatial and temporal dependence, and the transfer of regional carbon emissions is limited by the original type and stock of carbon emissions, showing growth inertia and path dependence. The findings of this paper can provide suggestions for planning and modifying policies to reduce carbon emissions in China's livestock industry.

Identification of molecular signatures in acute myocardial infarction based on integrative analysis of proteomics and transcriptomics.

BACKGROUND: Myocardial infarction (MI) is one of the most serious cardiovascular diseases, characterized by a rapid and irreversible decline in myocardial function. Early detection of patients with MI and prolonging the optimal therapeutic window of acute myocardial infarction (AMI) are particularly important. This study aimed to identify the diagnostic biomarkers and novel therapeutic targets for acute myocardial infarction. METHOD: We generated the AMI mouse models by ligating the proximal left anterior descending coronary artery. Six time points-Sham, AMI 10-min, 1-h, 6-h, 24-h, and 72-h-were chosen to examine the molecular changes that occur during the AMI process. RNA-seq and DIA-MS were performed on the infarcted left ventricular tissues of AMI mice at each time point. Co-expression pattern genes were screened from myocardial infarction samples at different time points by time-series analysis. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to examine these genes. Using the Interactive Gene/Protein Retrieval Tool (STRING) database, the protein-protein interaction network (PPI) was constructed and the hub genes were identified. In order to evaluate the diagnostic value of hub genes, a receiver operating characteristic (ROC) curve was constructed. An independent data set, GSE163772, confirmed the diagnostic value of hub genes further. RESULT: We obtained the expression profiles at different time points after the occurrence of heart failure through high-throughput sequencing, and found 167 genes with similar expression patterns through time series analysis. The immune response and immune-related pathways had the greatest enrichment of these genes. Among them, Itgb2 Syk, Tlr4, Tlr2, Itgax, and Lcp2 may play key roles as hub genes. Combined with the results of proteomic analysis, it was found that the expression of Coro1a in both omics increased with time. The results of external validation showed that TLR2, ITGAX, and LCP2 had good predictive ability for AMI diagnosis. CONCLUSION: Itgb2, Syk, Tlr4, Tlr2, Itgax, Lcp2 and Coro1a are considered to be the seven key genes significantly associated with AMI. Our results may provide potential targets for the prevention of adverse ventricular remodeling and the treatment of AMI.

Recent Advances in Asymmetric Catalysis Associated with B(C6F5)3.

The prevalence and significance of asymmetric catalysis in the modern medicinal industry has been witnessed in recent years, which have already been used to manufacture the (S)-Naproxen and the (S)-Propranolol. With matched specificities such as the Lewis acidity and steric bulk, B(C6F5)3 has gained accelerating attention on its application in asymmetric catalysis of Diels-Alder cycloaddition reactions, carbonyl-ene cyclization, and other various reactions, which have been demonstrated by the elegant examples from the most recent literature. Some significant progress in the reaction of indirect activation of substrates through in situ generation of numerous supramolecular catalysts from B(C6F5)3 based on Lewis-acid-assisted Lewis acid (LLA) or Lewis acid assisted Brønsted acid (LBA) strategies or the reaction promoted by cooperative actions of chiral co-catalysts and B(C6F5)3 which played a direct role on the activation of substrates have been demonstrated in this review.