ABSTRACT
OBJECTIVE: The aim of this study was to investigate the exact role of long non-coding RNA (lncRNA) RUNX1-IT1 in regulating the proliferation and apoptosis of hepatocellular carcinoma (HCC) cells, and to explore the possible underlying mechanism. PATIENTS AND METHODS: LncRNA RUNX1-IT1 expressions in paired HCC tissues (cancer and paired non-cancer tissues) (n=80) and HCC cell lines were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The effects of lncRNA RUNX1-IT1 on the proliferation and apoptosis of HCC cells were estimated by cell counting kit-8 (CCK-8) and tunnel assay, respectively. Furthermore, the association between lncRNA RUNX1-IT1 expression and the overall survival of patients was analyzed by the survival curve. RESULTS: The expression level of lncRNA RUNX1-IT1 significantly decreased in HCC tissues. The overexpression of lncRNA RUNX1-IT1 remarkably inhibited the proliferation and induced the apoptosis of HCC cells. On the contrary, the knockdown of lncRNA RUNX1-IT1 remarkably enhanced the ability of proliferation and repressed the apoptosis of the HCC cells. In addition, lower expression of lncRNA RUNX1-IT1 indicated the worse prognosis of HCC patients. CONCLUSIONS: We found that lncRNA RUNX1-IT1 played an important role in the development of HCC by participating in cell proliferation and apoptosis. Thus, lncRNA RUNX1-IT1 might be a powerful candidate as a prognostic biomarker and therapeutic target for HCC.
Subject(s)
Carcinoma, Hepatocellular/metabolism , Liver Neoplasms/metabolism , MAP Kinase Signaling System , Oncogene Proteins, Fusion/metabolism , RNA, Long Noncoding/metabolism , Apoptosis , Blotting, Western , Cell Line, Tumor , Cell Proliferation , Cells, Cultured , Humans , Liver/metabolism , Real-Time Polymerase Chain ReactionABSTRACT
The hemorheological behavior pre- and post-freezing and tissue survival area (TSA) of frostbitten hind feet in cold-acclimated rats (group CA) and the control (group C) were observed in order to go further into the mechanism of cold acclimation. The results indicated that blood viscosity and RBC aggregation tendency were lowered and RBC deformability improved obviously in CA rats as compared with those in group C (p<0.01). After the rats' hind feet were frozen, the hemorheological behavior in both group CA and C rats was changed abnormally and the changes were less serious in CA rats than those in C rats. The TSA of CA rats was much larger than that of C rats (p<0.01). The aforesaid results revealed that after rats were acclimated to cold, their resistance to freezing increased and their adaptive changes in hemorheological behavior occurred. The extent of abnormal changes in hemorheological behavior caused by freezing was reduced in CA rats, therefore the disorder microcirculation could be improved. These changes were beneficial to the frostbitten tissue to recovering and healing. So it is considered that in CA rats, the adaptive change in hemorheological behavior is one of the cause enhancing resistance to frostbite.
Subject(s)
Acclimatization/physiology , Frostbite/blood , Hemorheology/methods , Animals , Blood Flow Velocity , Blood Viscosity , Cold Temperature , Disease Models, Animal , Freezing , Hematocrit , Hindlimb/blood supply , Male , Rats , Rats, Wistar , Reference ValuesABSTRACT
OBJECTIVE: Research of the hair damage due to perming, combing and stretching can be of important value for forensic hair individual identification. METHODS: The normal human hairs were treated with perming combing and stretching, and the keratins of the damage hair were analysed by using SDS-PAGGE and laser densimeter. RESULTS: Perming, combing and stretching brought about hair damage; The keratins of the damage hair were obviously reduced at the rang of molecular weight of 67,000-43,000 dalton. CONCLUSION: The loss of the damage hair keratins were increased with the degree of the hair damage.
Subject(s)
Hair/chemistry , Keratins/metabolism , Adult , Female , Forensic Medicine , Hair/physiopathology , Hot Temperature/adverse effects , Humans , Keratins/analysisSubject(s)
Blood Coagulation , Frostbite/blood , Frostbite/therapy , Hypoxia/blood , Animals , Disease Models, Animal , Male , RabbitsABSTRACT
AIM: To correlate the injury of vascular endothelial cells during various pathological conditions with the change of vWF (von Willebrand Factor) in different VEC lines. METHODS: Flow cytometer(FCM) were used to defect the immunoflourescent stained vWF in pulmonary artery endothelial cells (PAEC) of pig and aortic endothelial cells(AEC) of rats. RESULTS: The positive rates of vWF in PAEC of pigs is similar with that in AEC of rats under normal condition, but it decreased differently after hypoxic or cold injury. It was very interesting that the mean fluorescence intensity of positive PAEC or AEC exposed to hypoxia or cold elevated significantly compared with those of control. CONCLUSIONS: The change of vWF in VEC can be used to evaluate the function of VEC under different stress.
Subject(s)
Endothelium, Vascular/metabolism , Peptide Fragments/metabolism , von Willebrand Factor/metabolism , Animals , Cell Hypoxia , Cold Temperature/adverse effects , Endothelium, Vascular/pathology , Female , In Vitro Techniques , Male , Rats , Rats, Wistar , Stress, Physiological/metabolism , SwineABSTRACT
Nine tetranucleotide short tandem repeat (STR) locus of D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820 and one Amelogenin loci were analyzed using multiplex PCR with fluorescent-labeled primers following capillary electrophoresis. This method is simple, accurate, sensitive and very useful for personal identification.
Subject(s)
Forensic Medicine/methods , Tandem Repeat Sequences , Genotype , Humans , Polymerase Chain ReactionABSTRACT
Studies were conducted to evaluate the application of D1S80, PM + DQA1 and CTT to Forensic Science. The methods used to type D1S80, DQA1 + PM and CTT are simple, accurate, sensitive, and also were useful for personal identification and paternity testing.
Subject(s)
DNA/genetics , Forensic Medicine/methods , HLA-DQ Antigens/genetics , DNA/isolation & purification , Gene Amplification , HLA-DQ alpha-Chains , HumansABSTRACT
Keratin contents of different hairs from the head, pubic, armpit and leg of one individual were analyzed by SDS-PAGGE. Samples were collected from 20 individuals. The results showed that the electrophoretic patterns of different body hairs of the same individual were almost identical. The relative contents of keratin composition from the same individual were tested by Laser Densimetic Scanning. No obvious difference was found. Electrophoretic keratin patterns and relative keratin contents of head hairs from different parts (crown, left side, right side, forehead and occiput) of the same individual were observed. No obvious difference was found. It is concluded the analysis of electrophoretic keratin pattern of human body hairs can be used as an important evidence for forensic individual identification.
Subject(s)
Forensic Medicine , Hair/chemistry , Keratins/analysis , Adult , Electrophoresis, Polyacrylamide Gel , Humans , MaleABSTRACT
The phenomenon of gene linkage and recombination may nearly be overlooked in paternity test of one single child, but it is likely encountered in paternity test of twin or more. In a case of paternity test, the results of 17 items including eight DNA loci were analyzed and the phenomenon of gene linkage and recombination was discussed in detail. This phenomenon should be brought into necessary attention in the paternity test.
Subject(s)
Genetic Linkage , Paternity , Recombination, Genetic , Blood Group Antigens/genetics , Female , Humans , Male , Parent-Child RelationsABSTRACT
Fragments of the heavy alpha-chain of IgA (IgA Fc alpha) were prepared and purified from the serum of patients with heavy chain disease. It was used to immunize BALB/c mice. The immunized mouse spleen cells were then fused with myeloma cells Sp2/O. Through successive recloning of the hybridoma cell lines by the limiting dilution method, four subclonal hybridoma cells (D2, E4, F5 and G10) capable of screening IgA Fc alpha were found. Intraperitoneal transplantation of these cells induced ascites in the BALB/c mice, from which four types of monoclonal antibodies (McAb-IgA Fc alpha) were obtained. The highest McAb titer was as high as 1:16348 by the hemagglutination inhibition (HAI) test. These McAb's reacted specifically with IgA Fc alpha, but not with IgG, IgM, IgD, kappa or lambda. Serum samples from 92 normal subjects and 73 patients with various diseases were tested for Ig genetic markers by means of HAI. The family trees of three positive cases were surveyed. Results showed that the McAb-IgA Fc alpha may be used to determine the genetic marker of IgA allotypes.
