ABSTRACT
Lanthanide-containing polyoxometalate (POM) is one of the presently most active groups as the properties of POM can be modified by incorporated transition metal ions. There are several reported transition metal ion contained POMs such as one Ln ion-POM with Keggin POM frameworks and a novel sandwich"-type, which consists of two open Wells-Dawson anions and three Ln ions [1-3]. In this data article, UV-vis spectra and FTIR spectra as well as the elemental analysis are shown for prepared ß-K7H8[Er3O3(SiW9O34)2]·25H2O and ß-K12H5[Sm3O3(SiW9O34)2]·18H2O, these results are intended to provide support for lanthanide-containing POM due to the lack of information on lanthanide-containing POM. The UV-vis spectra and FTIR spectra as well as the elemental analysis are shown for prepared ß-K7H8[Er3O3(SiW9O34)2]·25H2O and ß-K12H5[Sm3O3(SiW9O34)2]·18H2O were not reported in the article [4] are given. The data is related to the research article "Enhancing Electrofluorochromic Efficiency through C 30 H 31 N 6 + -Sensitized Layer-by-layer Polyoxometalate Films" [4].
ABSTRACT
Hydrogen sulfide (H2S) is an important gaseous signaling agent mediated by many physiological processes and diseases. In order to explore its role in biological signaling, much effort has been focused on developing organic fluorescent probes to image H2S. However, these downconversion H2S probes are impractical for bio-imaging beyond a certain depth because of the short tissue penetration of UV/visible light (as an excitation source). In most circumstance, these probes are also not suitable for long-term assay due to photo-bleaching. Herein, a new design to detect H2S based on the coumarin-hemicyanine (CHC1)-modified upconversion nanophosphors is reported. This inorganic-organic integrated nanoprobe is demonstrated to display a fast response time with a large ratiometric upconversion luminescence (UCL) enhancement, and extraordinary photo-stability. CHC1-UCNPs not only can be used for ratiometric UCL monitoring of pseudo-enzymatic H2S production in living cells, but can also be used to identify the risk of endotoxic shock through ratiometric UCL imaging of tissue and measurement of endogenous H2S levels in plasma. The first ratiometric UCL H2S nanoprobe reported here may be further developed as the next-generation diagnostic tool for the detection of inflammatory-related diseases.
Subject(s)
Hydrogen Sulfide/chemistry , Inflammation , Nanostructures/chemistry , Spectroscopy, Near-Infrared/methods , Animals , Carbocyanines/chemistry , Coumarins/chemistry , Disease Models, Animal , Fluorescent Dyes/chemistry , HeLa Cells , Humans , Lipopolysaccharides/chemistry , Luminescence , Magnetic Resonance Spectroscopy , Mice , Mice, Nude , Microscopy, Electron, Transmission , Nanoparticles/chemistry , Nanotechnology/methods , Shock, Septic/blood , Shock, Septic/diagnosis , Signal Transduction , Spectrophotometry, UltravioletABSTRACT
Hypochlorous acid (HOCl), a reactive oxygen species (ROS) produced by myeloperoxidase (MPO) enzyme-mediated peroxidation of chloride ions, acts as a key microbicidal agent in immune systems. However, misregulated production of HOCl could damage host tissues and cause many inflammation-related diseases. Due to its biological importance, many efforts have been focused on developing fluorescent probes to image HOCl in living system. Compared with those conventional fluorescent probes, up-conversion luminescence (UCL) detection system has been proven to exhibit a lot of advantages including no photo-bleaching, higher light penetration depth, no autofluorescence and less damage to biosamples. Herein, we report a novel water-soluble organic-nano detection system based on rhodamine-modified UCNPs for UCL-sensing HOCl. Upon the interaction with HOCl, the green UCL emission intensity in the detection system were gradually decreased, but the emissions in the NIR region almost have no change, which is very important for the ratiometric UCL detection of HOCl in aqueous solution. More importantly, RBH1-UCNPs could be used for the ratiometric UCL visualization of HOCl released by MPO-mediated peroxidation of chloride ions in living cells. This organic-nano system could be further developed into a novel next-generation imaging technique for bio-imaging HOCl in living system without background noise.
