[The Ultrasonographic Features of Female Reproductive System Extraosseous Ewing's Sarcoma].

OBJECTIVE: This study investigated the clinicopathological traits and ultrasound features of female reproductive system extraosseous Ewing's sarcoma (EES) and explored the diagnostic value of ultrasonography for this condition. METHODS: Cases of female pelvic EES diagnosed and treated at our hospital between June 2009 and June 2019 were included in this study. Pathology data and ultrasound manifestations were assessed retrospectively to summarize the clinical traits and ultrasound features of female reproductive system EES. Based on the results, recommendations for the ultrasonography-based diagnosis of this disease were proposed. RESULTS: During the 10-year study period, 13 female patients were diagnosed with EES in the pelvic cavity based on the results of postoperative pathology tests. The age of the patients ranged from 8 mouth to 40 years, and no patients demonstrated specific clinical symptoms. However, an examination of tumor biomarkers revealed that certain patients had elevated levels of CA125. In the 13 patients, 19 lesions were identified, including 16 that involved the reproductive system. The primary ultrasound manifestation was uneven, low-echo solidity or cystic solidity, exhibiting large size, irregular shape, and unclear boundary. A few patients had concurrent ascites. Although some lesions lacked blood supply, the blood supply of most lesions was medium to abundant, and the blood flow was mostly characterized by low resistant. Almost none of the lesions were definitively diagnosed preoperatively. CONCLUSIONS: Preoperative definitive diagnosis of EES in the female reproductive system remains a great clinical challenge. Although certain clinical traits and ultrasound features are associated with this disease, and color Doppler ultrasonography might provide vital information indicating the presence of EES, the final diagnosis still depends on the pathological test results of the patients.

Influence of pharmaceutical care on medication compliance and treatment effect and carotid atheromatous plaque in patients with hyperhpidemia / 中国药学杂志

OBJECTIVE: To explore the influence of pharmaceutical care on medication compliance and treatment effect and carotid atheromatous plaque in patients with hyperlipidemia.

[Comparative study of ultrasonography and magnetic resonance imaging in midline structures of fetal brain].

OBJECTIVE: To evaluate the accuracy of ultrasonography (US) and magnetic resonance imaging (MRI) in midline structures of fetal brain. METHODS: Thirty-two fetuses underwent US and MRI examinatiom. Imagings acquired from US and MRI were analysed, the results were compared with the final outcomes which were obtained after fetal birth or pathological study after induction of labor. RESULTS: There were 20 cases found abnormal in prenatal CNS ultrasonography (18 cases involving midline structures ). Among these 20 cases, 12 cases were normal in MRI and the fetuses were born in full-term without special problem, 8 cases were abnormal in MRI and fetal autopsy confirmed their abnormalities. Diagnostic accuracy rate of US was 93.8%, sensitivity was 100%, specificity was 87.5%, positive predictive value was 88.9%, negatvie predictive value was 100%. Diagnostic accuracy rate of MRI was 100%, modifying the US results in 4 cases. CONCLUSION: US and MRI examinations are important in fetal central nervous system. US could be the first line examination, and MRI is an important supplement because of the high diagnostic accuracy, especially in the image study of midline structures of fetal brain.

[Diagnostic value of power Doppler ultrasonography for Sirenomelia Seguence in prenatal].

OBJECTIVE: To study and discuss the diagnostic value and ultrasonographic characteristics of power doppler ultrasound in the prenatal diagnosis of Sirenomelia Seguence. METHODS: The abdominal aorta in two fetuses with sirenomelia seguence fetuses and in ten with nomal was reviewed and compared with two-dimensional power doppler ultrasound and three-dimensional power doppler ultrasound in prenatal. RESULTS: The abdominal aorta were showed to divid into renal arteries in the kidney level while two common iliac arteries in the pelvis in nomal fetuses with two-dimensional power doppler ultrasound and three-dimensional power doppler ultrasound; compared with the nomal, the abdominal aorta and whose branches in sirenomelia seguence were demonstrated as follows: 1) a large and deformed vascular coming from the high abdominal aorta, which was found to act as a umbilical artery by careful examination; 2) no bifurcation of renal arteries identified; 3) no bifurcation of two common iliac arteries identified; 4) the abdominal aorta changing into a narrow vascular after one deformed vascular separating from. CONCLUSIONS: Sirenomelia seguence fetuses has a characteristic change in two-dimensional power doppler ultrasound and three-dimensional power doppler ultrasound, which is helpful to improve the prenatal diagnosis of sirenomelia seguence.

Ursolic acid induces human hepatoma cell line SMMC-7721 apoptosis via p53-dependent pathway / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Ursolic acid (UA) is a ubiquitous molecule in the plant kingdom with specific anticancer effects that have been shown in vitro and in vivo. Although UA can inhibit the proliferation of liver cancer cells and induce apoptosis of many types of tumor cells, the molecular mechanism of its anti-hepatoma activity is still not well defined. The objective of this study was to investigate the inhibitory effect and mechanisms of UA on the human hepatoma cell line SMMC-7721.</p><p><b>METHODS</b>After treatment with UA, the growth inhibition of SMMC-7721 cells was assessed by MTT assay. Cells were also evaluated by flow cytometric analysis, Wright-Giemasa staining, Hoechst 33258 staining and transmission electron microscope after they were induced by UA. DNA microarray technology was used to investigate the gene expression pattern of SMMC-7721 cells exposed to UA 40 micromol/L. The molecular mechanism of cells death was analyzed by real-time RT-PCR and Western blotting.</p><p><b>RESULTS</b>The proliferation of SMMC-7721 cells was significantly inhibited in a dose- and time-dependent manner after UA treatment. UA induced cell cycle arrest and apoptosis. The DNA microarray analysis indicated that 64 genes were found to be markedly up- or down-expressed, including GDF15, SOD2, ATF3, and fos. The result of Western blotting showed the apoptotic proteins p53 and Bax were up-regulated while the anti-apoptotic protein Bcl-2 was down-regulated. Real-time RT-PCR confirmed UA could up-regulate the mRNA expressions of GDF15, SOD2, ATF3 and down-regulate the mRAN expression of fos. Meanwhile these effects were partly blocked by pretreatment with the p53 inhibitor Pft-alpha.</p><p><b>CONCLUSION</b>Activation of the p53 pathway is involved in UA inhibition of SMMC-7721 human hepatocellular carcinoma cell growth and induction of apoptosis.</p>