[Multiple pattern inverse water quality modeling approach for predicting the water quality consequence of Lake Chenghai water elevation control].

A water quality model was developed through incorporating the water surface elevation and water quality data of Lake Chenghai into the CE-QUAL-W2 computational platform. The model integrates the water surface elevation and water quality into a holistic dynamic system based on the data of Lake Chenghai, and was calibrated against observed data using a multiple pattern inverse water quality modeling technology, which was driven by a robust genetic algorithm (GA). After the model was calibrated, it was used to produce robust predictions of the lake water quality in response to various water elevation controlled scenarios. The model established a basis for quantifying the water quality responses under uncertainty, and is valuable for supporting effective and reliable management decision making. The results of this research suggest that various water elevation control scenarios only result in insignificant water quality improvement in terms of TN, TP, and COD concentrations, therefore, it does not recommend to consider water elevation control to be the major water quality management option for Lake Chenghai.

Simultaneous determination of chlorogenic acid, forsythin and arctiin in Chinese traditional medicines preparation by reversed phase-HPLC.

A reversed phase (RP)-HPLC method was established for simultaneous determination of chlorogenic acid, arctiin and forsythin in Yinqiao Jiedu Granules, which was a commonly used Chinese herbal medical preparation for treatment of rheum ailments. The determination was based on a gradient elution (A: 1% acetic acid, pH=3.0, B: methanol) on a C18 column and an automatic wavelength switching program, where 325 nm was used for chlorogenic acid and 280 nm for arctiin and forsythin, respectively. Good linearities were obtained over the range of 2-200 mg.l(-1) for the 3 objective compounds. The spike recoveries were within 96.0-97.9%.

RP-HPLC determination of puerarin in Chinese traditional medicinal preparations containing pueraria.

Puerarin in a Gegen(Pueraria)-based Chinese traditional medicinal preparations, Ganmao Qingre Granules, from four different pharmaceutical manufacturers and in two different dosage forms, were determined using RP-HPLC with methanol-5 mmol l(-1) KH(2)PO(4) (pH = 4.0) (V:V = 27:73) as mobile phase and UV detection at 248 nm. Ultrasonication and reflux were compared as pretreatment procedures for the sample. Linear range over 0.2-200 microg ml(-1) of puerarin was obtained, and the limit of detection was 0.1 microg ml(-1). Recovery was within 99.7-103%.