[SERS spectra of serum from rash].

The SERS spectra of serums from the healthy persons and rash patients were measured. In the serum of rash, the band of amide I appeared at 1648 cm(-1), while this peak vanished in healthy serums. The relative intensity of 637 cm(-1) assigned to the gauche conformation of C S increased 23% and the band at 725 cm(-1) corresponding to anti-conformation decreases 60%. These indicate that the structure of the protein has changed in serums from the rash patients. The relative intensity at 1449 cm(-1) assigned to the lipids increased nearly one time. The band at 1099 cm(-1) assigned to the Man-D vanished, indicating that the contents of lipids, glucide and protein increased in rash patients. These results may offer a powerful experiment basis for rash diagnosis and biochemistry mechanism study. The multivariae ura statistical methods of principal component analysis (PCA) were also used to analyze the SERS of the serums from healthy persons and rash patients, it can be seen that the regional distribution of rash is wider than healthy volunteers, showing that these serums can be discriminated by PCA.

[SERS spectra of serum from diabetic].

The SERS spectra were measured from normal and the diabetic serum. In the diabetic serum, the band of amide II shifted to 1 585 cm(-1) and the relative intensity increased 14%, while the relative intensity of 593 cm(-1) which belongs to amide VI reduced 33%. For the protein side chain, the band at 1 368 cm(-1) assigned to the "buried" tryptophan shifted to 1 365 cm(-1) of the "exposed" and the relative intensity reduced 59%. The relative intensity of 635 cm(-1) assigned to the gauche conformation of the C-S decrased 15% and the band at 725 cm(-1) increased 58%. These indicate that the structure of the protein changed in the diabetic serum. The relative intensity at 1 449 cm(-1) assigned to the lipids characteristic increased 58%. The relative intensity of the glucide characteristic at 1 331, 1 099 and 740 cm(-1) increased 35%, 100% and 62%, respectively. So it is indicated that the content of lipids, glucide and protein increased in diabetic. These results may offer a powerful experimental basis for diabetes diagnosis and biochemistry mechanism study.

Raman spectra of single cell from gastrointestinal cancer patients.

AIM: To explore the difference between cancer cells and normal cells, we investigated the Raman spectra of single cells from gastrointestinal cancer patients. METHODS: All samples were obtained from 30 diagnosed as gastrointestinal cancer patients. The flesh tumor specimen is located in the center of tumor tissue, while the normal ones were 5 cm away from the outside tumor section. The imprint was put under the micros-cope and a single cell was chosen for Raman measurement. All spectra were collected at confocal Raman micro-spectroscopy (British Renishaw) with NIR 780 nm laser. RESULTS: We measured the Raman spectra of several cells from gastrointestinal cancer patients. The result shows that there exists the strong line at 1 002 /cm with less half-width assigned to the phenylalanine in several cells. The Raman lines of white cell were lower and less, while those of red cell were not only higher in intensity and more abundant, but also had a particular C-N breathing stretching band of pyrrole ring at 1 620-1 540 /cm. The line at 1 084 /cm assigned to phosphate backbone of DNA became obviously weaker in cancer cell. The Raman spectra of stomach cancer cells were similar to those of normal cells, but the Raman intensity of cancer cells was much lower than that of normal cells, and even some lines disappear. The lines of enteric cancer cells became weaker than spectra above and many lines disappeared, and the cancer cells in different position had different fluorescence intensity. CONCLUSION: The Raman spectra of several cells from cancer patients show that the structural changes of cancer cells happen and many bonds rupture so that the biological function of cells are lost. The results indicate that Raman spectra can offer the experiment basis for the cancer diagnosis and treatment.

[Raman spectra of cell from breast cancer patients].

Raman spectra of normal and cancer cell from breast cancer patients a represented. It is reported that all of the Ramanlines become weak. The intensities of Raman lines 782 and 1 084 cm(-1) of DNA phosphate group and 1 155 and 1 262 cm(-1) of deoxyribosephosphate decrease. The spectral lines 812 cm(-1) of A-type DNA and 979 and 668 cm(-1) disappear, and the 905 cm(-1) peak is shifted to lower frequency by 6 cm(-1). This means that the phosphate backbone of DNA is destroyed to a certain extent and the fissiparity of cancer cell can't be controlled effectively. In addition, the authors found a kind of Raman line 960 cm(-1) concerning calcificationand sclerosis of cancer cell. The results indicate that Raman spectra may offer the experiment basis for the cancer diagnosis and treatment.

[Temperature dependence of collagen by Raman spectra].

Raman spectra of collagen I at different temperature were obtained. There was no change at 1003 cm(-1) line, 1302 cm(-1) line moved to a higher wave number, but other lines moved to lower wave number when temperature increased. In addition, the authors observed the temperature dependence of Raman intensity and four denaturation points at 0, 40, 68 and 90 degrees C respectively. The points at 40 and 68 degrees C are in agreement with the experimental data by DSC and SHG. The point at 0 degrees C might be frozen transition; the point at 90 degrees C might be related to the damage of secondary structure. When heated to 150 degrees C, the Raman intensity of all bands decreased rapidly and many lines disappeared.

[Temperature dependence study of calf thymus DNA by Raman spectra].

In this paper, the authors obtained Raman spectra of DNA in fiber and aqueous solution at different temperatures. These spectra revealed that the vibration of bases and phosphate group were influenced by varying temperature. Adenine was the most sensitive to varying temperature in all of the vibrational modes. The wave numbers of most of the vibrational modes decreased as the temperature increased except for the band at 1101 cm(-1), and the shift of wave numbers mainly concentrated upon the beginning point of denaturation at 70 degrees C. The temperature dependence of Raman intensity was discussed, and two peaks were obtained at 38 and 82 degrees C, respectively. The results obtained by Raman spectra were in agreement with experimental data by DSC at 82 degrees C. The peak at 38 degrees C was related to the biological activity region of DNA function.

[Resonance Raman spectra of single red-cell from human blood].

Resonance Raman spectra of single red-cell from human blood are presented by different laser sources. It is reported that there is 1002 cm(-1) line of insensitive conformation of phenylalanine aromatic ring stretching and 1620 cm(-1) line of C-N breathing stretching band of pyrrole ring, which cause strong and sharp resonance lines when excited by 782 nm laser source. They are weaker and the intensity of other lines of high wave number is large and clear when excited by 514 nm laser source. But the intensity of lines of low wave number is strong and clear when excited by 782 nm laser source. At the same time, the authors got Raman spectra lines at different times after taking blood under the same laser source. When using 782 nm laser source, there is no difference except for 1601 cm(-1). There are a lot of weakened lines and lines shifting about 4-10 cm(-1) toward low wave number. The results indicate that Raman spectra may offer the experimental basis for studying structure, function and variability of single red-cell.