Integrating egg albumin sodium and lignosulfonate nanoparticles food packing for significantly improved multifunctional characteristics.

In food packaging, sodium lignosulfonate nanoparticles (SLS NPs) showed significant antibacterial properties, antioxidant and UV barrier activities. Herein, the SLS NPs were synthesized via a sustainable green method and were added into egg albumin/sodium alginate mixture (EA/SA) to fabricate a safe, edible EA/SA/SNPs food packaging. A composite film EA/SA/SNP was examined microstructurally and physicochemically. The mechanical characteristics, UV protection, water resistance, and the composite film's thermal stability were all enhanced by the inclusion of SLS NPs, and water vapor permeability reduced by 44 %. This composite film exhibited robust antioxidative properties with DPPH and ABTS free radical scavenging rates reaching 76.84 % and 92.56 %, and effective antimicrobial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) with antibacterial rates reaching 98.25 % and 97.13 % for the positively charged nanoparticles interacting with the cell membrane. Freshness tests showed that the EA/SA/SNPs packaging film could delay the quality deterioration of fresh tomatoes. This composite film can slow down spoilage bacteria proliferation and prolongsfood's preservation period by eight days at ambient temperature.

3D printing of fish-scale derived hydroxyapatite/chitosan/PCL scaffold for bone tissue engineering.

In the field of bone tissue repair, the treatment of bone defects has always posed a significant challenge. In recent years, the advancement of bone tissue engineering and regenerative medicine has sparked great interest in the development of innovative bone grafting materials. In this study, a novel hydroxyapatite (HA) material was successfully prepared and comprehensively characterized. Antimicrobial experiments and biological evaluations were conducted to determine its efficacy. Based on the aforementioned research findings, 3D printing technology was employed to fabricate HA/chitosan (CS)/ polycaprolactone (PCL) scaffolds. The composition of the scaffold materials was confirmed through X-ray diffractometer (XRD) and Fourier Transform Infrared Spectroscopy (FT-IR) tests, while the influence of different HA ratios on the scaffold surface morphology was observed. Additionally, antimicrobial experiments demonstrated the favorable antimicrobial activity of the scaffolds containing 30%HA + 5%CS + PCL. Furthermore, the water contact angle measurements confirmed the superhydrophilicity of the scaffolds. Finally, the excellent bioactivity and ability to promote tissue regeneration of the scaffolds were further confirmed by in vitro and in vivo experiments. This study provides new options for future repair and regeneration of bone tissue and clinical applications.

Polysaccharide- and protein-based edible films combined with microwave technology for meat preservation.

To reduce food-borne bacterial infection caused by food spoilage, developing highly efficient food packing film is still an urgent need for food preservation. Herein, microwave-assisted antibacterial nanocomposite films CaO2@PVP/EA/CMC-Na (CP/EC) were synthesized using waste eggshell as precursor, egg albumen (EA) and sodium carboxymethylcellulose (CMCNa) as matrix by casting method. The size of CaO2@PVP (CP) nanoparticles with monodisperse spherical structures was 100-240 nm. When microwave and CP nanoparticles (0.05 mg/mL) were treated for 5 min, the mortality of E. coli and S. aureus could reach >97 %. Under microwave irradiation (6 min), the bactericidal rate of 2.5 % CP/EC film against E. coli and S. aureus reached 98.6 % and 97.2 %, respectively. After adding CP nanoparticles, the highest tensile strength (TS) and elongation at break (EB) of CP/EC film reached 19.59 MPa and 583.43 %, respectively. At 18 °C, the proliferation of bacterial colonies on meat can be significantly inhibited by 2.5 % CP/EC film. Detailed characterization showed that the excellent meat preservation activity was due to the synergistic effect of dynamic effect generated by ROS and thermal effect of microwave. This study provides a promising approach for the packaging application of polysaccharide- and protein-based biomass nanocomposite antibacterial edible films.

Tracheal regeneration and mesenchymal stem cell augmenting potential of natural polyphenol-loaded gelatinmethacryloyl bioadhesive.

Hydrogels incorporating natural biopolymer and adhesive substances have extensively been used to develop bioactive drugs and to design cells encapsulating sturdy structure for biomedical applications. However, the conjugation of the adhesive in most hydrogels is insufficient to maintain long-lasting biocompatibility inadequate to accelerate internal organ tissue repair in the essential native cellular microenvironment. The current work elaborates the synthesis of charged choline-catechol ionic liquid (BIL) adhesive and a hydrogel with an electronegative atom rich polyphenol (PU)-laden gelatinmethacryloyl (GelMA) to improve the structural bioactivities for in vivo tracheal repair by inducing swift crosslinking along with durable mechanical and tissue adhesive properties. It was observed that bioactive BIL and PU exhibited potent antioxidant (IC 50 % of 7.91 µg/mL and 24.55 µg/mL) and antibacterial activity against E. coli, P. aeruginosa and S. aureus. The novel integration of photocurable GelMA-BIL-PU revealed outstanding mechanical strength, biodegradability and sustained drug release. The in vitro study showed exceptional cell migration and proliferation in HBECs, while in vivo investigation of the GelMA-BIL-PU hydrogel on a rat's tracheal model revealed remarkable tracheal reconstruction, concurrently reducing tissue inflammation. Furthermore, the optimized GelMA-BIL-PU injectable adhesive bioink blend demonstrated superior MSCs migration and proliferation, which could be a strong candidate for developing stem cell-rich biomaterials to address multiple organ defects.

Self-Assembled and Multilayer-Overlapped ESM-PDA@rGO Nanofilm-Based Flexible Wearable Sensor for Real-Time Body Temperature Monitoring.

There is an urgent need for wearable sensors that continuously monitor human physiological conditions in real time. Herein, an ESM-PDA@rGO-based flexible wearable temperature sensor was successfully constructed by integrating an eggshell membrane (ESM) with reduced graphene oxide (rGO) through dopamine (DA) polymerization. Depending on the "bridge effect" of diversified polydopamine (PDA) chains, on the one hand, a staggered arrangement of PDA-rGO frameworks and a lot of conductive pathways were produced and acted as an active layer. On the other hand, PDA-rGO frameworks were linked with ESM by PDA chains as a flexible sensing nanofilm. As a result, these mechanical merits of the ESM-PDA@rGO exhibited a 1.8-fold increase in Young' s modulus and 1.4-fold increase in tensile strength. Thereby, the conformability and performance of the temperature sensor were greatly enhanced, showing excellent sensitivity (-2.23%/°C), good linearity (R2 = 0.979), as well as stability. Especially, the flexible sensing nanofilm is evolved from the staggered arrangement of PDA-rGO frameworks, which endows it with rapid response (only 4-8 s), high resolution (0.1 °C), as well as excellent long-term durability (10 weeks). More importantly, the temperature sensor demonstrates insensitivity to bending deformation, ensuring reliable wearing stability. The sensor allows for online, real-time monitoring of human body temperature, encompassing both core (forehead, temple, cochlea, and exhale gas) and shell (palm and back of the hand, fingertip, and instep) temperatures. Particularly, it can accurately assess minor changes in peripheral body temperature before and after exercise, and it is capable of mapping daily patterns of body temperatures. The developed temperature sensor will provide us new materials design concepts and holds considerable promise in the fields of e-skin, disease surveillance, prediction, and diagnose.

Hydrophilic ZnO/C nanocomposites with superior adsorption, photocatalytic, and photo-enhanced antibacterial properties for synergistic water purification.

Owing to the numerous potential applications of ZnO nanomaterials, the development of ZnO-based nanocomposites has become of great scientific interest in various fields. In this paper, we are reporting the fabrication of a series of ZnO/C nanocomposites through a simple "one-pot" calcination method under three different temperatures, 500 ℃, 600 ℃, and 700 ℃, with samples labeled as ZnO/C-500, -600, and -700, respectively. All samples exhibited adsorption capabilities and photon-activated catalytic and antibacterial properties, with the ZnO/C-700 sample showing superior performance among the three. The carbonaceous material in ZnO/C is key to expanding the optical absorption range and improving the charge separation efficiency of ZnO. The remarkable adsorption property of the ZnO/C-700 sample was demonstrated using Congo red dye, and is credited to its good hydrophilicity. It was also found to exhibit the most notable photocatalysis effect due to its high charge transfer efficiency. The hydrophilic ZnO/C-700 sample was also examined for antibacterial effects both in vitro (against Escherichia coli and Staphylococcus aureus) and in vivo (against MSRA-infected rat wound model), and it was observed to exhibit synergistic killing performance under visible-light irradiation. A possible cleaning mechanism is proposed on the basis of our experimental results. Overall, this work presents a facile way of synthesizing ZnO/C nanocomposites with outstanding adsorption, photocatalysis, and antibacterial properties for the efficient treatment of organic and bacterial contaminants in wastewater.

C-doped ZnO nanocomposites molecularly imprinted photoelectrochemical sensor for ultrasensitive and selective detection of oxytetracycline in milk.

Antibiotic monitoring remains vital to ensure human health and safety in the environment and foods. As the most popular detection method, photoelectrochemical (PEC) sensor can achieve rapid and accurate detection of antibiotics with the advantages of high sensitivity, easy-to-preparation process, as well as high selectivity. Herein, an extremely-efficient visible-light responsible ZnO/C nanocomposite was prepared and combined with acetylene black (as an enhanced conductive matrix), and the electron migration efficiency was greatly accelerated. Meanwhile, a molecularly imprinted polymer obtained by electrical agglomeration was conjugated as a specific recognizing site for target. Furthermore, the as-prepared rMIP-PEC sensor showed a low detection limit (8.75 pmol L-1, S/N = 3) in a wide linear detection range of 0.01-1000 nmol L-1 for oxytetracycline (OTC), with excellent selectivity and long-term stability. Our work shed light on applying C-doped ZnO semiconductor and molecularly imprinted polymer as photoelectric active sensing materials for rapid and accurate analysis of antibiotics in foods and environment.

Rapid and sensitive dilute-and-shoot analysis using LC-MS-MS for identification of multi-class psychoactive substances in human urine.

The emergence of new psychoactive substances currently exceeding a thousand is rapidly changing substance prevalence patterns and straining the methods used for detection, most of which are suitable only for a single class of substances. This study presents a rapid and facile dilute-and-shoot system operated in conjunction with an optimized liquid chromatographic separation system for the high-sensitivity detection of substances across a range of substance classes with 3 isotopes used only. The proposed method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) is able to identify 68 substance and their metabolites in urine samples as small as 50 µL. Optimal chromatographic conditions including 95% water/methanol ratio with 0.1% added formic acid and a prolonged LC gradient run-time (15 min) improved the peak shape of polar compounds and enhanced signal strength by 5%. Under 4-fold dilution, all analytes were within 80-120% of tolerance response levels, indicating that the matrix effect was insignificant. In experiments, the limit of detection (LOD) ranged from 0.05 to 0.5 ng mL-1, while the coefficient of determination (R2) was > 0.9950. The retention time shift of each peak remained at < 2% with an inter-day relative standard deviation (RSD) of 0.9-14.9% and intra-day RSD of 1.1%- 13.8%. The rapid dilute-and-shoot presents a high-sensitivity, significant stability, robustness and reproducibility without serious interference. To demonstrate the effectiveness of the system, 532 urine samples were collected from suspected drug abusers, and the proposed method was used for rapid analysis. Of these samples, 79.5% contained between one and twelve analytes, and 12.4% tested positive for new psychoactive substances, mostly derivatives of amphetamine and synthetic cathinones. The study presents a high-sensitivity analytic system that is capable of detecting substances from multiple classes and can be used for effective monitoring of substance prevalence in urine.

The influence of substrates addition order on colorimetric assay based on MnO2 nanocubes: A novel turn-off H2O2 assay strategy in water-soak foods.

Herein, novel MnO2 nanocubes were facilely synthesized by manipulating the dosage of KMnO4 and l-Dopa with the aid of ultrasound. The as-prepared MnO2 nanocubes exhibited interesting oxidation activity which was influenced by the addition order of substrates (H2O2 and 3,3,5,5-tetramethylbenzidine (TMB)). After the mechanism study, we found that H2O2 and TMB can be competitively oxidized by MnO2 nanocubes, which was different from the peroxidase- and oxidase-like activities. According to the discovery, a novel turn-off H2O2 assay method based on MnO2 nanocubes was established, where H2O2 was firstly incubated with MnO2 nanocubes for 3 min and then TMB was added for the instantaneous chromogenic reaction. In addition to the shorter operation time, the colorimetric results were less affected by temperature and unchanged within 30 min without terminating reaction. Moreover, the method showed ultra-high sensitivity with low limit of detection (0.027 µmol L-1) and acceptable reliability for H2O2 assay in water-soak foods.

A dual aperture (mesoporous and macroporous) system loaded with cell-free fat extract to optimize bone regeneration microenvironment.

Injured bone regeneration requires a systemically and carefully orchestrated series of events involving inflammation, angiogenesis, and osteogenesis. Thus, we designed a multifunctional cell-supporting and drug-retarding dual-pore system: cell-free fat extract (Ceffe)-mesoporous silica nanoparticle (MSN)@poly(lactic-co-glycolic acid) (PLGA) (Ceffe-MSN@PLGA) to mimic the developmental spatial structure, the microenvironment of bone regeneration and integration during injured bone regeneration. In this system, a macroporous scaffold (pore size 200-250 µm) of PLGA is combined with mesoporous MSN (pore size 2-50 nm), aiming at realizing the slow release of Ceffe. Besides, PLGA and MSN are used to recruit the temporary support of cells that are able to degrade simultaneously with bone regeneration and provide space for bone tissue regeneration. And the Ceffe isolated from fresh human adipose tissue has a therapeutic effect in regulating the important functions of early inflammatory cell transformation, neovascularization and eventual osteogenic differentiation. Our results suggest that the mesoporous and macroporous Ceffe-MSN@PLGA system represents a promising strategy to better fit the regeneration of injured bone tissue.

Recent advances in luminescence and aptamer sensors based analytical determination, adsorptive removal, degradation of the tetracycline antibiotics, an overview and outlook.

Tetracycline antibiotics (TCs), one of the important antibiotic groups, have been widely used in human and veterinary medicines. Their residues in foodstuff, soil and sewage have caused serious threats to food safety, ecological environment and human health. Here, we reviewed the potential harms of TCs residues to foodstuff, environment and human beings, discussed the luminescence and aptamer sensors based analytical determination, adsorptive removal, and degradation strategies of TCs residues from a recent 5-year period. The advantages and intrinsic limitations of these strategies have been compared and discussed, the potential challenges and opportunities in TCs residues degradation have also been deliberated and explored.

ZnO/C nanocomposite grafted molecularly imprinted polymers as photoelectrochemical sensing interface for ultrasensitive and selective detection of chloramphenicol.

Nanomaterials-based photoelectrochemical (PEC) detection is becoming a rapidly-developing analytical technique in chemical and biological assays due to its unique advantages of easy miniaturization, high sensitivity, and rapid turnaround time. Herein, a molecularly imprinted polymer-assisted PEC sensor based on ZnO/C nanocomposite was successfully fabricated for the highly sensitive and selective determination of chloramphenicol (CAP). Benefiting from the hydrophilic functional groups (-OH, -COOH) and large surface area of bio-templated ZnO/C nanocomposite, the tight grafting of MIP with excellent recognition ability on substrate is easier and more stable than traditional PEC sensor, thus significantly increasing the performance. Under optimal conditions, the PEC sensor exhibited significant CAP detection performance in the range of 0.01-5000 ng mL-1 with a detection LOD of 5.08 pg mL-1 (S/N = 3) and successfully applied to the detection of CAP in milk sample. Our results show that ZnO/C nanocomposite and MIP can act as an efficient photo-responsible matrix to fabricate PEC sensor, providing important application potentials for pollutants control in food and environment.

An ultrasensitive cathodic electrochemiluminescence immunoassay for thrombomodulin based on Ru(bpy)32+ encapsulated by MIL-NH2-101(Al) nanocomposites.

The rapid and reliable determination of thrombomodulin (TM) is of great significance for the diagnosis of disseminated intravascular coagulation, thrombosis and others. This work exhibits an electrochemiluminescent (ECL) sensor, which was prepared using Ru(bpy)32+ encapsulated by MIL-NH2-101(Al) nanocomposites for the sensitive detection of the new-thrombus marker thrombomodulin (TM) for the first time. Specifically, on one hand, with the advantages of high specific surface area, large hollow porous structure and favorable biocompatibility, MIL-NH2-101(Al) could load a large amount of luminescent Ru(bpy)32+ and thereby greatly enhance the ECL signal of the immunosensor. On the other hand, K2S2O8 is used as co-reactant to form a reduction-oxidation ECL system for cathodic ECL detection with strong anti-interference capacity. The experimental results show that the ECL signal intensity of the Ru(bpy)32+@MIL-NH2-101(Al)-based immunosensor decreased with the immunocapturing of TM, exhibiting a linear detection concentration ranging from 1 × 10-5 to 10 µg mL-1 and the limit of detection (LOD) of 8.2 × 10-6 µg mL-1 (S/N = 3). With its ideal stability, selectivity and reproducibility, the proposed ECL immunosensor can provide excellent aid and shows great promise for the detection of TM.

A fish-scale derived multifunctional nanofiber membrane for infected wound healing.

The rapid development of modern medicine has put forward new requirements for wound infection healing methods in clinical treatment. Despite the great achievements made in the research and development of various types of dressings in recent years, yet there is still a challenge of multifunctional dressings for effective wound treatment. Herein, a multifunctional nanofibrous membrane was prepared by encapsulating NIR-adsorbed CuS (FSC/CuS) nanoparticles, polyvinylpyrrolidone (PVP) and polyvinyl butyral (PVB) with electrospun fish scale collagen. During the evaluation of wound healing, four parameters, including hemostasis time, inflammatory response, cell proliferation, and tissue remodeling, were considered. The results of H&E, Masson and immunohistochemical staining showed that the synergistic effect of composite nanofibers and near-infrared light can inhibit the inflammatory response, promote the proliferation and migration of fibroblasts and keratinocytes, rebuild new tissues, form well-dispersed collagen fibers, etc. It was shown that the FSC/CuS NPs combined with an NIR-driven experimental group exhibited excellent performance in accelerating wound healing in these stages. This kind of nanofibrous scaffold prepared with fish scale and NIR-absorbing agents will have broad application prospects in the healing of infected wounds.

Designing waste Bioresource-derived value-added Nanohybrids for efficient photocatalysis water treatment.

Although photocatalysis with ultraviolet-visible (UV-vis) light has made considerable advances, it is limited by the low efficiency of UV-vis energy conversion. To overcome this problem, UV-vis light can be replaced with near-infrared (NIR) light. Herein, we coupled eggshell-derived CaCO3 with a NIR-absorbing CuSe semiconductor and fabricated an insulator-based heterojunction structure. In application case studies of 4-nitrophenol (4-NP) and bacteria, the nanocomposites showed enhanced photocatalysis activity under NIR light induction. A first-principles calculation indicated that photoexcited electrons could transfer from the conduction band of CuSe to the conduction band of CaCO3. The main reactive species generated by the photocatalysis were ·CO3-, and ·OH free radicals. The antibacterial mechanisms of photocatalysis on the cell permeability and DNA layers of the bacterial cells were also revealed. Besides providing novel perspectives and mechanistic understanding of the fabrication of NIR light-driven photocatalysts, this study demonstrates the valorization of eggshell bio-wastes in environmental remediation.

Eggshell membrane-mimicking multifunctional nanofiber for in-situ skin wound healing.

Eggshell membrane is a naturally-occurring protective barrier layer for chickens' incubation and shows the close similarity with extracellular matrix. To fully explore and utilize its' structure and active components via a mimicking way will be of great interest for wounds healing. Herein, the well-dispersed CuS nanoparticles were prepared by using eggshell membranes as templates with strong near-infrared absorption and photothermal properties. Furthermore, the as-prepared solution was combined with polyvinyl pyrrolidone and chitosan-derived fluorescent carbon dots for the mimetic synthesis of multifunctional nanofibrous membrane by a hand-held electrospinning device, which has the merits of in-situ operation, the extracellular matrix (ECM)-like architecture, hemostatic, radical scavenging, antibacterial, as well as accelerated healing of skin injury, etc. The electrospun-nanofiber membrane with optimal addition of 100 mg/L CuS nanoparticles was confirmed to be noncytotoxic on human fibroblasts and showed strong antibacterial activities against S. aureus and E. coli under NIR irradiation (980 nm). In addition, the radical scavenging ability was also proved by DPPH experiments. The animal experiments revealed that the nanofiber membrane could accelerate the wound healing process. The work lays down a simple and environmentally-friendly approach for the fabrication and development of promising wound healing materials in skin tissue engineering applications.

Monitoring Phthalates in Maternal and Cord Blood: Implications for Prenatal Exposure and Birth Outcomes.

Although many phthalates are endocrine-disrupting chemicals that are associated with adverse birth outcomes, the relationship between maternal phthalate exposure and birth outcomes is not yet conclusive. The objective of the present study was to investigate the association between prenatal exposure to phthalates in human maternal and cord blood and birth outcomes of the infants. Sixty-five mother-infant pairs were recruited in Taipei City and New Taipei City, and birth outcomes of the infants were recorded. Twelve phthalate metabolites were measured in maternal and cord blood samples. The mean of mono-ethyl phthalate, mono-isobutyl phthalate (MiBP), mono-n-butyl phthalate (MnBP), and mono-(2-ethylhexyl) phthalate (MEHP) was relatively higher than that of the other metabolites in both maternal and infant blood. There was a significant difference (p < 0.05) for mono-methyl phthalate (MMP) and MnBP between the maternal blood and cord blood of male infants. Mono-benzyl phthalate (MBzP), MMP, MiBP, and ∑di-2-ethylhexyl phthalate (∑DEHP) in maternal blood were inversely correlated with the anogenital index (AGI) of male infants, with a p value between 0.011 and 0.033. Mono-n-octyl phthalate, MMP, MiBP, MnBP, and MBzP were positively correlated with the AGI of female infants, with a p value between 0.001 and 0.034. Cord blood levels of MnBP, mono-(2-ethyl-5-oxohexyl)-phthalate, MEHP, and ∑DEHP were found to be inversely associated with head circumference in all the infants, adjusted for gestational age. Phthalate monoesters are potentially estrogenic and antiandrogenic chemicals. Longitudinal follow-up of the present study population could help clarify the long-term impact of phthalates on growth and the health effects of background exposure levels. Environ Toxicol Chem 2022;41:715-725. © 2022 SETAC.

Fabricating Dual-Functional Plasmonic-Magnetic Au@MgFe2O4 Nanohybrids for Photothermal Therapy and Magnetic Resonance Imaging.

Bifunctional nanohybrids possessing both plasmonic and magnetic functionalities are of great interest for biomedical applications owing to their capability for simultaneous therapy and diagnostics. Herein, we fabricate a core-shell structured plasmonic-magnetic nanocomposite system that can serve as a dual-functional agent due to its combined photothermal therapeutic and magnetic resonance imaging (MRI) functions. The photothermal activity of the hybrid is attributed to its plasmonic Au core, which is capable of absorbing near-infrared (NIR) light and converting it into heat. Meanwhile, the magnetic MgFe2O4 shell exerts its ability to act as a MRI contrast agent. Our in vivo studies using tumor-bearing mice demonstrated the nanohybrids' excellent photothermal and MRI properties. As a photothermal therapeutic agent, the nanohybrids were able to dramatically shrink solid tumors in mice through NIR-induced hyperthermia. As T 2-weighted MRI contrast agents, the nanohybrids were found capable of substantially reducing the MRI signal intensity of the tumor region at 10 min postinjection. With their dual plasmonic-magnetic functionality, these Au@MgFe2O4 nanohybrids hold great promise not only in the biomedical field but also in the areas of catalysis and optical sensing.

Injectable hydrogel for postoperative synergistic photothermal-chemodynamic tumor and anti-infection therapy.

Tumor surgery is usually accompanied by neoplasm residual, tissue defects, and multi-drug resistant bacterial infection, causing high tumor recurrence, low survival rate, and chronic wounds. Herein, a light-activated injectable hydrogel based on bioactive nanocomposite system is developed by incorporating Ag2S nanodots conjugated Fe-doped bioactive glass nanoparticles (BGN-Fe-Ag2S) into biodegradable PEGDA and AIPH solution for inhibiting tumor growth, treating bacterial infection, and promoting wound healing. Under laser irradiation, the photothermal effect mediated by Ag2S nanodots would trigger the decomposition of AIPH, generating alkyl radicals to initiate the gelation of PEGDA. The in-situ gelatinized hydrogel, with outstanding photothermal effect and chemodynamic effect derived from the doped Fe in BGN-Fe-Ag2S, can not only eliminate multidrug-resistant bacteria but also efficiently ablated tumor during treatment. Moreover, the hydrogel significantly accelerated wound healing with more skin appendages in the full-thickness cutaneous wounds model because of the hydrolysis of bioactive glass. These results manifest that this multifunctional hydrogel is a suitable biomaterial to inhibit tumor proliferation and overcome tissue bacterial infection after surgical removal of tumors.