[Clinical application of Meek micrograft technique].

OBJECTIVE: To explore the clinical application of the transplantation of the Meek autograft. METHODS: Meek autografts were transplanted on the full-thickness wounds of 10 patients, including 9 cases of burn and 1 case of necrofascitis, with the area of 8.6% (2% - 28%) of the total body surface, and the average of the area was. Meek skin graft was placed on the wounds immediately after escharectomy (in 4 cases), 4 - 16 days after tangential excision (in 4 cases), or on the clean wound granulation 35 - 45 days after the injury (in 2 cases). 108 pieces of extending gauze of different rates were used. As comparison, Stamp-like grafts, micrografts, or net-like graft were used in different places of the same bodies in 5, 4, and 1 case respectively. RESULTS: The operation time of Meek micrograft technique was 9 - 60 minutes. The survival rate of the Meek graft was 93% (100% in 8 cases for, 80% in 1 case, and 50% in 1 case). The operation time of the other operation techniques was 30 - 240 minutes. The average survival rate of the other operation techniques was 86% (100% in 2 cases, 90% - 98% in 4 cases, 80% in 3 cases, and 50% in 1 case). CONCLUSION: Meek autograft is suitable for all of the full-thickness wound except bone-exposure wound, including the wound of major burns. This technique uses less skin to repair the wound and the time for epithelization is shorter than that of the traditional methods. The staff and time needed for the operation are less. The outcome is good and the healed wound is fine-looking.

[Effect of expression of platelet-derived growth factor B gene on blood vessel reconstruction after tissue engineering skin grafting].

OBJECTIVE: To study the effect of PDGF on dermal blood vessel reconstruction by transplanted tissue-engineering skin containing PDGF-B gene to rats. METHODS: The recombined eukaryotic expression vector, pcDNA3.1-hPDGF-B, was constructed and transfected into fibroblasts mediated by LipofectAMINE. Keratinocytes + acellular dermal matrix (group A), keratinocytes + acellular dermal matrix + fibroblasts (group B), keratinocytes + acellular dermal matrix + fibroblasts with PDGF gene (group C) were recombined respectively, then transplanted them to rat dorsum and evaluated the reconstruction of blood vessels in the dermis after 2, 4, 6 week postoperation. RESULTS: In 2-4 weeks after skin grafting the vascularization rate in group C was higher than that of group B and group A. The vascularization rates in all groups had no significant differences in six weeks (P > 0.05). CONCLUSION: PDGF-B gene plays an important role in reconstruction of blood vessels in the dermis at early tissue-engineering skin grafting, which ensures the take of grafted tissue-engineering skin.