ABSTRACT
Biodesulfurization under haloalkaline conditions requires limiting oxygen and additional energy in the system to deliver high mixing quality control. This study considers biodesulfurization in an airlift bioreactor with uniform microbubbles generated by a fluidic oscillation aeration system to enhance the biological desulfurization process and its hydrodynamics. Fluidic oscillation aeration in an airlift bioreactor requires minimal energy input for microbubble generation. This aeration system produced 81.87% smaller average microbubble size than the direct aeration system in a bubble column bioreactor. The biodesulfurization phase achieved a yield of 94.94% biological sulfur, 84.91% biological sulfur selectivity, and 5.06% sulfur oxidation performance in the airlift bioreactor with the microbubble strategy. The biodesulfurization conditions of thiosulfate via Thioalkalivibrio versutus D306 are revealed in this study. The biodesulfurization conditions in the airlift bioreactor with the fluidic oscillation aeration system resulted in the complete conversion of thiosulfate with 27.64% less sulfate production and 10.34% more biological sulfur production than in the bubble column bioreactor. Therefore, pleasant hydrodynamics via an airlift bioreactor mechanism with microbubbles is favored for biodesulfurization under haloalkaline conditions.
ABSTRACT
Biological desulfurization offers several remarkably environmental advantages of operation at ambient temperature and atmospheric pressure, no demand of toxic chemicals as well as the formation of biologically re-usable sulfur (S0), which has attracted increasing attention compared to conventionally physicochemical approaches in removing hydrogen sulfide from sour gas. However, the low biomass of SOB, the acidification of process solution, the recovery of SOB, and the selectivity of bio-S0 limit its industrial application. Therefore, more efforts should be made in the improvement of the BDS process for its industrial application via different research perspectives. This review summarized the recent research advances in the microbial capture of hydrogen sulfide from sour gas based on strain modification, absorption enhancement, and bioreactor modification. Several efficient solutions to limitations for the BDS process were proposed, which paved the way for the future development of BDS industrialization.
ABSTRACT
Haloalkaliphilic Thioalkalivibrio, a dominant genus for sulfide removal, has attracted growing interest. However, the bacterial biological response to this process's final product, sulfate, has not been well-studied. Here, thiosulfate oxidation and sulfur formation by T. versutus D301 were being enhanced with increasing sulfate supply. With the addition of 0.73 M sulfate, the thiosulfate utilization rate and sulfur production were improved by 68.1% and 120.1% compared with carbonate-grown control at the same salinity (1.8 M). For sulfate-grown cells, based on metabolic analysis, the downregulation of central carbon metabolism indicated that sulfate triggered a decrease in energy conservation efficiency. Additionally, the gene expression analysis further revealed that sulfate induced the inhibition of sulfur to sulfate oxidation, causing the upregulation of thiosulfate to sulfur oxidation for providing cells with additional energy. This study enhances researchers' understanding regarding the sulfate effect on the bio-desulfurization process and presents a new perspective of optimizing the biotechniques.
